PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms
- Autores
- Amorós Morales, Leslie Cinthya; Marchesini, Abril; Gómez Bergna, Santiago Manuel; García Fallit, Matías; Tongiani, Silvana E.; Vásquez, Larisa; Ferrelli, María Leticia; Videla-Richardson, Guillermo A.; Candolfi, Marianela; Romanowski, Víctor; Pidre, Matías Luis
- Año de publicación
- 2023
- Idioma
- español castellano
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Baculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculoviruses takes on particular relevance. To achieve this goal, we developed a highly versatile baculoviral transfer vector generation system called PluriBAC. The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows enough versatility to combine more than four different promoters, genes of interest, and terminator sequences. Here, we report not only the rational design of the PluriBAC system but also its use for the generation of baculoviral reporter vectors applied to different fields of biotechnology. We demonstrated that recombinant AcMNPV baculoviruses generated with the PluriBAC system were capable of infecting Spodoptera frugiperda larvae. On the other hand, we found that the recombinant budded virions (BV) generated using our system were capable of transducing different types of tumor and normal cells both in vitro and in vivo. Our findings suggest that the PluriBAC system could constitute a versatile tool for the generation of insecticide and gene therapy vectors.
Instituto de Biotecnología y Biología Molecular - Materia
-
Biología
baculovirus expression vectors
golden gate assembly
bio-inputs
gene therapy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/160011
Ver los metadatos del registro completo
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PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platformsAmorós Morales, Leslie CinthyaMarchesini, AbrilGómez Bergna, Santiago ManuelGarcía Fallit, MatíasTongiani, Silvana E.Vásquez, LarisaFerrelli, María LeticiaVidela-Richardson, Guillermo A.Candolfi, MarianelaRomanowski, VíctorPidre, Matías LuisBiologíabaculovirus expression vectorsgolden gate assemblybio-inputsgene therapyBaculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculoviruses takes on particular relevance. To achieve this goal, we developed a highly versatile baculoviral transfer vector generation system called PluriBAC. The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows enough versatility to combine more than four different promoters, genes of interest, and terminator sequences. Here, we report not only the rational design of the PluriBAC system but also its use for the generation of baculoviral reporter vectors applied to different fields of biotechnology. We demonstrated that recombinant AcMNPV baculoviruses generated with the PluriBAC system were capable of infecting Spodoptera frugiperda larvae. On the other hand, we found that the recombinant budded virions (BV) generated using our system were capable of transducing different types of tumor and normal cells both in vitro and in vivo. Our findings suggest that the PluriBAC system could constitute a versatile tool for the generation of insecticide and gene therapy vectors.Instituto de Biotecnología y Biología Molecular2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/160011spainfo:eu-repo/semantics/altIdentifier/issn/1999-4915info:eu-repo/semantics/altIdentifier/doi/10.3390/v15101984info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:41:52Zoai:sedici.unlp.edu.ar:10915/160011Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:41:52.557SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms |
| title |
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms |
| spellingShingle |
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms Amorós Morales, Leslie Cinthya Biología baculovirus expression vectors golden gate assembly bio-inputs gene therapy |
| title_short |
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms |
| title_full |
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms |
| title_fullStr |
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms |
| title_full_unstemmed |
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms |
| title_sort |
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms |
| dc.creator.none.fl_str_mv |
Amorós Morales, Leslie Cinthya Marchesini, Abril Gómez Bergna, Santiago Manuel García Fallit, Matías Tongiani, Silvana E. Vásquez, Larisa Ferrelli, María Leticia Videla-Richardson, Guillermo A. Candolfi, Marianela Romanowski, Víctor Pidre, Matías Luis |
| author |
Amorós Morales, Leslie Cinthya |
| author_facet |
Amorós Morales, Leslie Cinthya Marchesini, Abril Gómez Bergna, Santiago Manuel García Fallit, Matías Tongiani, Silvana E. Vásquez, Larisa Ferrelli, María Leticia Videla-Richardson, Guillermo A. Candolfi, Marianela Romanowski, Víctor Pidre, Matías Luis |
| author_role |
author |
| author2 |
Marchesini, Abril Gómez Bergna, Santiago Manuel García Fallit, Matías Tongiani, Silvana E. Vásquez, Larisa Ferrelli, María Leticia Videla-Richardson, Guillermo A. Candolfi, Marianela Romanowski, Víctor Pidre, Matías Luis |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Biología baculovirus expression vectors golden gate assembly bio-inputs gene therapy |
| topic |
Biología baculovirus expression vectors golden gate assembly bio-inputs gene therapy |
| dc.description.none.fl_txt_mv |
Baculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculoviruses takes on particular relevance. To achieve this goal, we developed a highly versatile baculoviral transfer vector generation system called PluriBAC. The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows enough versatility to combine more than four different promoters, genes of interest, and terminator sequences. Here, we report not only the rational design of the PluriBAC system but also its use for the generation of baculoviral reporter vectors applied to different fields of biotechnology. We demonstrated that recombinant AcMNPV baculoviruses generated with the PluriBAC system were capable of infecting Spodoptera frugiperda larvae. On the other hand, we found that the recombinant budded virions (BV) generated using our system were capable of transducing different types of tumor and normal cells both in vitro and in vivo. Our findings suggest that the PluriBAC system could constitute a versatile tool for the generation of insecticide and gene therapy vectors. Instituto de Biotecnología y Biología Molecular |
| description |
Baculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculoviruses takes on particular relevance. To achieve this goal, we developed a highly versatile baculoviral transfer vector generation system called PluriBAC. The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows enough versatility to combine more than four different promoters, genes of interest, and terminator sequences. Here, we report not only the rational design of the PluriBAC system but also its use for the generation of baculoviral reporter vectors applied to different fields of biotechnology. We demonstrated that recombinant AcMNPV baculoviruses generated with the PluriBAC system were capable of infecting Spodoptera frugiperda larvae. On the other hand, we found that the recombinant budded virions (BV) generated using our system were capable of transducing different types of tumor and normal cells both in vitro and in vivo. Our findings suggest that the PluriBAC system could constitute a versatile tool for the generation of insecticide and gene therapy vectors. |
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2023 |
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2023 |
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