Biotechnological applications of occlusion bodies of Baculoviruses

Autores
López, M.G.; Diez, M.; Alfonso, Victoria; Taboga, Oscar Alberto
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed.
Fil: López, M.G.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Diez, M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Materia
BACULOVIRUS
BEVS
FUSIONS
OCCLUSION BODIES
POLYHEDRIN
RECOMBINANT PROTEINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99563
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Biotechnological applications of occlusion bodies of BaculovirusesLópez, M.G.Diez, M.Alfonso, VictoriaTaboga, Oscar AlbertoBACULOVIRUSBEVSFUSIONSOCCLUSION BODIESPOLYHEDRINRECOMBINANT PROTEINShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed.Fil: López, M.G.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Diez, M.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaSpringer2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99563López, M.G.; Diez, M.; Alfonso, Victoria; Taboga, Oscar Alberto; Biotechnological applications of occlusion bodies of Baculoviruses; Springer; Applied Microbiology and Biotechnology; 102; 16; 8-2018; 6765-67740175-7598CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-018-9130-2info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-018-9130-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:16:07Zoai:ri.conicet.gov.ar:11336/99563instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:16:07.698CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biotechnological applications of occlusion bodies of Baculoviruses
title Biotechnological applications of occlusion bodies of Baculoviruses
spellingShingle Biotechnological applications of occlusion bodies of Baculoviruses
López, M.G.
BACULOVIRUS
BEVS
FUSIONS
OCCLUSION BODIES
POLYHEDRIN
RECOMBINANT PROTEINS
title_short Biotechnological applications of occlusion bodies of Baculoviruses
title_full Biotechnological applications of occlusion bodies of Baculoviruses
title_fullStr Biotechnological applications of occlusion bodies of Baculoviruses
title_full_unstemmed Biotechnological applications of occlusion bodies of Baculoviruses
title_sort Biotechnological applications of occlusion bodies of Baculoviruses
dc.creator.none.fl_str_mv López, M.G.
Diez, M.
Alfonso, Victoria
Taboga, Oscar Alberto
author López, M.G.
author_facet López, M.G.
Diez, M.
Alfonso, Victoria
Taboga, Oscar Alberto
author_role author
author2 Diez, M.
Alfonso, Victoria
Taboga, Oscar Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv BACULOVIRUS
BEVS
FUSIONS
OCCLUSION BODIES
POLYHEDRIN
RECOMBINANT PROTEINS
topic BACULOVIRUS
BEVS
FUSIONS
OCCLUSION BODIES
POLYHEDRIN
RECOMBINANT PROTEINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed.
Fil: López, M.G.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Diez, M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
description The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99563
López, M.G.; Diez, M.; Alfonso, Victoria; Taboga, Oscar Alberto; Biotechnological applications of occlusion bodies of Baculoviruses; Springer; Applied Microbiology and Biotechnology; 102; 16; 8-2018; 6765-6774
0175-7598
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99563
identifier_str_mv López, M.G.; Diez, M.; Alfonso, Victoria; Taboga, Oscar Alberto; Biotechnological applications of occlusion bodies of Baculoviruses; Springer; Applied Microbiology and Biotechnology; 102; 16; 8-2018; 6765-6774
0175-7598
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-018-9130-2
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-018-9130-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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