PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
- Autores
- Montero Jimenez, Marjorie; Amante, Francisco L.; Fenoy, Gonzalo Eduardo; Scotto, Juliana; Azzaroni, Omar; Marmisollé, Waldemar Alejandro
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas - Materia
-
Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/154186
Ver los metadatos del registro completo
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PEDOT-polyamine-based organic electrochemical transistors for monitoring protein bindingMontero Jimenez, MarjorieAmante, Francisco L.Fenoy, Gonzalo EduardoScotto, JulianaAzzaroni, OmarMarmisollé, Waldemar AlejandroQuímicaOrganic electrochemical transistorsConducting polymersProtein bindingPEDOTThe fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/154186enginfo:eu-repo/semantics/altIdentifier/issn/2079-6374info:eu-repo/semantics/altIdentifier/doi/10.3390/bios13020288info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:39:55Zoai:sedici.unlp.edu.ar:10915/154186Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:39:56.24SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding |
title |
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding |
spellingShingle |
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding Montero Jimenez, Marjorie Química Organic electrochemical transistors Conducting polymers Protein binding PEDOT |
title_short |
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding |
title_full |
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding |
title_fullStr |
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding |
title_full_unstemmed |
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding |
title_sort |
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding |
dc.creator.none.fl_str_mv |
Montero Jimenez, Marjorie Amante, Francisco L. Fenoy, Gonzalo Eduardo Scotto, Juliana Azzaroni, Omar Marmisollé, Waldemar Alejandro |
author |
Montero Jimenez, Marjorie |
author_facet |
Montero Jimenez, Marjorie Amante, Francisco L. Fenoy, Gonzalo Eduardo Scotto, Juliana Azzaroni, Omar Marmisollé, Waldemar Alejandro |
author_role |
author |
author2 |
Amante, Francisco L. Fenoy, Gonzalo Eduardo Scotto, Juliana Azzaroni, Omar Marmisollé, Waldemar Alejandro |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Química Organic electrochemical transistors Conducting polymers Protein binding PEDOT |
topic |
Química Organic electrochemical transistors Conducting polymers Protein binding PEDOT |
dc.description.none.fl_txt_mv |
The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas |
description |
The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/154186 |
url |
http://sedici.unlp.edu.ar/handle/10915/154186 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/altIdentifier/issn/2079-6374 info:eu-repo/semantics/altIdentifier/doi/10.3390/bios13020288 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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