PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding

Autores
Montero Jimenez, Marjorie; Amante, Francisco L.; Fenoy, Gonzalo Eduardo; Scotto, Juliana; Azzaroni, Omar; Marmisollé, Waldemar Alejandro
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Materia
Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/154186
Acceder
id SEDICI_385523238b007af424b1db8f6f44506f
oai_identifier_str oai:sedici.unlp.edu.ar:10915/154186
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling PEDOT-polyamine-based organic electrochemical transistors for monitoring protein bindingMontero Jimenez, MarjorieAmante, Francisco L.Fenoy, Gonzalo EduardoScotto, JulianaAzzaroni, OmarMarmisollé, Waldemar AlejandroQuímicaOrganic electrochemical transistorsConducting polymersProtein bindingPEDOTThe fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/154186enginfo:eu-repo/semantics/altIdentifier/issn/2079-6374info:eu-repo/semantics/altIdentifier/doi/10.3390/bios13020288info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:31:44Zoai:sedici.unlp.edu.ar:10915/154186Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:31:44.453SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
spellingShingle PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
Montero Jimenez, Marjorie
Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT
title_short PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_full PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_fullStr PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_full_unstemmed PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_sort PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
dc.creator.none.fl_str_mv Montero Jimenez, Marjorie
Amante, Francisco L.
Fenoy, Gonzalo Eduardo
Scotto, Juliana
Azzaroni, Omar
Marmisollé, Waldemar Alejandro
author Montero Jimenez, Marjorie
author_facet Montero Jimenez, Marjorie
Amante, Francisco L.
Fenoy, Gonzalo Eduardo
Scotto, Juliana
Azzaroni, Omar
Marmisollé, Waldemar Alejandro
author_role author
author2 Amante, Francisco L.
Fenoy, Gonzalo Eduardo
Scotto, Juliana
Azzaroni, Omar
Marmisollé, Waldemar Alejandro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT
topic Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT
dc.description.none.fl_txt_mv The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
description The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/154186
url http://sedici.unlp.edu.ar/handle/10915/154186
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2079-6374
info:eu-repo/semantics/altIdentifier/doi/10.3390/bios13020288
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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