Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>

Autores
Carasi, Paula; Díaz, Mariángeles; Racedo, Silvia María; De Antoni, Graciela Liliana; Urdaci, María C.; Serradell, María de los Ángeles
Año de publicación
2014
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Lactobacillus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/67064
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/67064
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network_name_str SEDICI (UNLP)
spelling Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>Carasi, PaulaDíaz, MariángelesRacedo, Silvia MaríaDe Antoni, Graciela LilianaUrdaci, María C.Serradell, María de los ÁngelesCiencias ExactasLactobacillusLactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.Facultad de Ciencias Exactas2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/67064spainfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2014/208974/info:eu-repo/semantics/altIdentifier/doi/10.1155/2014/208974info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:10:10Zoai:sedici.unlp.edu.ar:10915/67064Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:10:10.582SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
spellingShingle Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
Carasi, Paula
Ciencias Exactas
Lactobacillus
title_short Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_full Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_fullStr Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_full_unstemmed Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_sort Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
dc.creator.none.fl_str_mv Carasi, Paula
Díaz, Mariángeles
Racedo, Silvia María
De Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
author Carasi, Paula
author_facet Carasi, Paula
Díaz, Mariángeles
Racedo, Silvia María
De Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
author_role author
author2 Díaz, Mariángeles
Racedo, Silvia María
De Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Lactobacillus
topic Ciencias Exactas
Lactobacillus
dc.description.none.fl_txt_mv Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.
Facultad de Ciencias Exactas
description Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/67064
url http://sedici.unlp.edu.ar/handle/10915/67064
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2014/208974/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2014/208974
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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