Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiat...
- Autores
- Santos da Paixão, Flávia Regina; Huarte Bonnet, Carla; Ribeiro Silva, Cárita Souza; Mascarin, Gabriel Moura; Fernandes, Éverton K. K.; Pedrini, Nicolás
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compacted and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia, thus they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both UV-B radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker, and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the sixteen genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx, the peroxisome biogenesis factors Mrpex5 and Mrpex14/17, and the photoprotection genes Mrlac1, Mrlac2, and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii, and confirmed that due to its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.
Facultad de Ciencias Exactas
Instituto de Investigaciones Bioquímicas de La Plata - Materia
-
Ciencias Exactas
Bioquímica
entomopathogenic fungi
UV-B radiation
thermotolerance
oxidative stress
gene expression - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/124323
Ver los metadatos del registro completo
id |
SEDICI_333c8cc6c9307ffbce2316f58f0df82f |
---|---|
oai_identifier_str |
oai:sedici.unlp.edu.ar:10915/124323 |
network_acronym_str |
SEDICI |
repository_id_str |
1329 |
network_name_str |
SEDICI (UNLP) |
spelling |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial DifferentiationSantos da Paixão, Flávia ReginaHuarte Bonnet, CarlaRibeiro Silva, Cárita SouzaMascarin, Gabriel MouraFernandes, Éverton K. K.Pedrini, NicolásCiencias ExactasBioquímicaentomopathogenic fungiUV-B radiationthermotoleranceoxidative stressgene expression<i>Metarhizium</i> species fungi are able to produce resistant structures termed microsclerotia, formed by compacted and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia, thus they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both UV-B radiation and heat of microsclerotia of <i>Metarhizium robertsii</i> strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker, and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the sixteen genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes <i>MrcatA</i>, <i>MrcatP</i>, and <i>Mrgpx</i>, the peroxisome biogenesis factors <i>Mrpex5</i> and <i>Mrpex14/17</i>, and the photoprotection genes <i>Mrlac1</i>, <i>Mrlac2</i>, and <i>Mrlac3</i>. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in <i>M. robertsii</i>, and confirmed that due to its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.Facultad de Ciencias ExactasInstituto de Investigaciones Bioquímicas de La Plata2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/124323enginfo:eu-repo/semantics/altIdentifier/issn/2673-6128info:eu-repo/semantics/altIdentifier/doi/10.3389/ffunb.2021.654737info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:53Zoai:sedici.unlp.edu.ar:10915/124323Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:53.65SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation |
title |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation |
spellingShingle |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation Santos da Paixão, Flávia Regina Ciencias Exactas Bioquímica entomopathogenic fungi UV-B radiation thermotolerance oxidative stress gene expression |
title_short |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation |
title_full |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation |
title_fullStr |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation |
title_full_unstemmed |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation |
title_sort |
Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation |
dc.creator.none.fl_str_mv |
Santos da Paixão, Flávia Regina Huarte Bonnet, Carla Ribeiro Silva, Cárita Souza Mascarin, Gabriel Moura Fernandes, Éverton K. K. Pedrini, Nicolás |
author |
Santos da Paixão, Flávia Regina |
author_facet |
Santos da Paixão, Flávia Regina Huarte Bonnet, Carla Ribeiro Silva, Cárita Souza Mascarin, Gabriel Moura Fernandes, Éverton K. K. Pedrini, Nicolás |
author_role |
author |
author2 |
Huarte Bonnet, Carla Ribeiro Silva, Cárita Souza Mascarin, Gabriel Moura Fernandes, Éverton K. K. Pedrini, Nicolás |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Bioquímica entomopathogenic fungi UV-B radiation thermotolerance oxidative stress gene expression |
topic |
Ciencias Exactas Bioquímica entomopathogenic fungi UV-B radiation thermotolerance oxidative stress gene expression |
dc.description.none.fl_txt_mv |
<i>Metarhizium</i> species fungi are able to produce resistant structures termed microsclerotia, formed by compacted and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia, thus they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both UV-B radiation and heat of microsclerotia of <i>Metarhizium robertsii</i> strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker, and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the sixteen genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes <i>MrcatA</i>, <i>MrcatP</i>, and <i>Mrgpx</i>, the peroxisome biogenesis factors <i>Mrpex5</i> and <i>Mrpex14/17</i>, and the photoprotection genes <i>Mrlac1</i>, <i>Mrlac2</i>, and <i>Mrlac3</i>. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in <i>M. robertsii</i>, and confirmed that due to its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence. Facultad de Ciencias Exactas Instituto de Investigaciones Bioquímicas de La Plata |
description |
<i>Metarhizium</i> species fungi are able to produce resistant structures termed microsclerotia, formed by compacted and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia, thus they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both UV-B radiation and heat of microsclerotia of <i>Metarhizium robertsii</i> strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker, and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the sixteen genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes <i>MrcatA</i>, <i>MrcatP</i>, and <i>Mrgpx</i>, the peroxisome biogenesis factors <i>Mrpex5</i> and <i>Mrpex14/17</i>, and the photoprotection genes <i>Mrlac1</i>, <i>Mrlac2</i>, and <i>Mrlac3</i>. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in <i>M. robertsii</i>, and confirmed that due to its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/124323 |
url |
http://sedici.unlp.edu.ar/handle/10915/124323 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/2673-6128 info:eu-repo/semantics/altIdentifier/doi/10.3389/ffunb.2021.654737 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
reponame_str |
SEDICI (UNLP) |
collection |
SEDICI (UNLP) |
instname_str |
Universidad Nacional de La Plata |
instacron_str |
UNLP |
institution |
UNLP |
repository.name.fl_str_mv |
SEDICI (UNLP) - Universidad Nacional de La Plata |
repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
_version_ |
1844616178034540544 |
score |
13.070432 |