Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiat...

Autores
Santos da Paixão, Flávia Regina; Huarte Bonnet, Carla; Ribeiro Silva, Cárita Souza; Mascarin, Gabriel Moura; Fernandes, Éverton K. K.; Pedrini, Nicolás
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compacted and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia, thus they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both UV-B radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker, and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the sixteen genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx, the peroxisome biogenesis factors Mrpex5 and Mrpex14/17, and the photoprotection genes Mrlac1, Mrlac2, and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii, and confirmed that due to its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.
Facultad de Ciencias Exactas
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Ciencias Exactas
Bioquímica
entomopathogenic fungi
UV-B radiation
thermotolerance
oxidative stress
gene expression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/124323

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial DifferentiationSantos da Paixão, Flávia ReginaHuarte Bonnet, CarlaRibeiro Silva, Cárita SouzaMascarin, Gabriel MouraFernandes, Éverton K. K.Pedrini, NicolásCiencias ExactasBioquímicaentomopathogenic fungiUV-B radiationthermotoleranceoxidative stressgene expression<i>Metarhizium</i> species fungi are able to produce resistant structures termed microsclerotia, formed by compacted and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia, thus they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both UV-B radiation and heat of microsclerotia of <i>Metarhizium robertsii</i> strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker, and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the sixteen genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes <i>MrcatA</i>, <i>MrcatP</i>, and <i>Mrgpx</i>, the peroxisome biogenesis factors <i>Mrpex5</i> and <i>Mrpex14/17</i>, and the photoprotection genes <i>Mrlac1</i>, <i>Mrlac2</i>, and <i>Mrlac3</i>. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in <i>M. robertsii</i>, and confirmed that due to its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.Facultad de Ciencias ExactasInstituto de Investigaciones Bioquímicas de La Plata2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/124323enginfo:eu-repo/semantics/altIdentifier/issn/2673-6128info:eu-repo/semantics/altIdentifier/doi/10.3389/ffunb.2021.654737info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:53Zoai:sedici.unlp.edu.ar:10915/124323Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:53.65SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation
title Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation
spellingShingle Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation
Santos da Paixão, Flávia Regina
Ciencias Exactas
Bioquímica
entomopathogenic fungi
UV-B radiation
thermotolerance
oxidative stress
gene expression
title_short Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation
title_full Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation
title_fullStr Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation
title_full_unstemmed Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation
title_sort Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets From <i>Metarhizium robertsii</i>, and Molecular and Ultrastructural Changes During Microsclerotial Differentiation
dc.creator.none.fl_str_mv Santos da Paixão, Flávia Regina
Huarte Bonnet, Carla
Ribeiro Silva, Cárita Souza
Mascarin, Gabriel Moura
Fernandes, Éverton K. K.
Pedrini, Nicolás
author Santos da Paixão, Flávia Regina
author_facet Santos da Paixão, Flávia Regina
Huarte Bonnet, Carla
Ribeiro Silva, Cárita Souza
Mascarin, Gabriel Moura
Fernandes, Éverton K. K.
Pedrini, Nicolás
author_role author
author2 Huarte Bonnet, Carla
Ribeiro Silva, Cárita Souza
Mascarin, Gabriel Moura
Fernandes, Éverton K. K.
Pedrini, Nicolás
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Bioquímica
entomopathogenic fungi
UV-B radiation
thermotolerance
oxidative stress
gene expression
topic Ciencias Exactas
Bioquímica
entomopathogenic fungi
UV-B radiation
thermotolerance
oxidative stress
gene expression
dc.description.none.fl_txt_mv <i>Metarhizium</i> species fungi are able to produce resistant structures termed microsclerotia, formed by compacted and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia, thus they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both UV-B radiation and heat of microsclerotia of <i>Metarhizium robertsii</i> strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker, and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the sixteen genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes <i>MrcatA</i>, <i>MrcatP</i>, and <i>Mrgpx</i>, the peroxisome biogenesis factors <i>Mrpex5</i> and <i>Mrpex14/17</i>, and the photoprotection genes <i>Mrlac1</i>, <i>Mrlac2</i>, and <i>Mrlac3</i>. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in <i>M. robertsii</i>, and confirmed that due to its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.
Facultad de Ciencias Exactas
Instituto de Investigaciones Bioquímicas de La Plata
description <i>Metarhizium</i> species fungi are able to produce resistant structures termed microsclerotia, formed by compacted and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia, thus they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both UV-B radiation and heat of microsclerotia of <i>Metarhizium robertsii</i> strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker, and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the sixteen genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes <i>MrcatA</i>, <i>MrcatP</i>, and <i>Mrgpx</i>, the peroxisome biogenesis factors <i>Mrpex5</i> and <i>Mrpex14/17</i>, and the photoprotection genes <i>Mrlac1</i>, <i>Mrlac2</i>, and <i>Mrlac3</i>. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in <i>M. robertsii</i>, and confirmed that due to its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/124323
url http://sedici.unlp.edu.ar/handle/10915/124323
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2673-6128
info:eu-repo/semantics/altIdentifier/doi/10.3389/ffunb.2021.654737
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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