Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii

Autores
Santos Da Paixao, Flavia Regina; Muniz, E.R.; Barreto, L. P.; Bernardo, C. C.; Mascarin, G. M.; Luz, C.; Fernandes, Éverton
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The thermotolerance of oil-based conidial formulations of Metarhizium anisopliae s.l. (IP 46) and Metarhizium robertsii (ARSEF 2575) were investigated. Conidia of IP 46 or ARSEF 2575 were suspended in different adjuvants and exposed to 45 ± 0.2°C for 4, 6, 8 or 24 h; their viability was then assessed after 48 h incubation at 27 ± 1°C. Conidia heated in pure mineral or vegetable oil exhibited mean relative viability exceeding 70% after 8 h of heat exposure, whereas low germination (≤20%) was observed when conidia were heated in water (Tween 80® 0.01%), carboxymethyl cellulose gel or emulsifiable oils (Graxol® or Assist®) and exposed to heat for 6 or 8 h. In addition, conidia of IP 46 suspended in either pure mineral or canola oil and exposed to heat for 48 h had moderate viability, 57% or 41%, respectively. Unstable oil-in-water emulsions showed a higher percentage of conidia incorporated into oil micellae, while the stable emulsions had higher percentage of conidia outside the oil micellae. The thermotolerance of conidia formulated in stable emulsions, however, did not differ from that of conidia formulated in unstable emulsions. The present study highlights possibilities to alleviate the deleterious effects of heat stress towards Metarhizium spp. conidia applied for controlling arthropod pests and vectors through oil-based formulations.
Fil: Santos Da Paixao, Flavia Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina. Universidade Federal de Goiás; Brasil
Fil: Muniz, E.R.. Universidade Federal de Goiás; Brasil
Fil: Barreto, L. P.. Universidade Federal de Goiás; Brasil
Fil: Bernardo, C. C.. Universidade Federal de Goiás; Brasil
Fil: Mascarin, G. M.. Universidade Federal de Goiás; Brasil
Fil: Luz, C.. Universidade Federal de Goiás; Brasil
Fil: Fernandes, Éverton. Universidade Federal de Goiás; Brasil
Materia
Adjuvants
Conidial Thermotolerance
Entomopathogenic Fungi
Oil-In-Water Emulsion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48987
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48987
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsiiSantos Da Paixao, Flavia ReginaMuniz, E.R.Barreto, L. P.Bernardo, C. C.Mascarin, G. M.Luz, C.Fernandes, ÉvertonAdjuvantsConidial ThermotoleranceEntomopathogenic FungiOil-In-Water Emulsionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The thermotolerance of oil-based conidial formulations of Metarhizium anisopliae s.l. (IP 46) and Metarhizium robertsii (ARSEF 2575) were investigated. Conidia of IP 46 or ARSEF 2575 were suspended in different adjuvants and exposed to 45 ± 0.2°C for 4, 6, 8 or 24 h; their viability was then assessed after 48 h incubation at 27 ± 1°C. Conidia heated in pure mineral or vegetable oil exhibited mean relative viability exceeding 70% after 8 h of heat exposure, whereas low germination (≤20%) was observed when conidia were heated in water (Tween 80® 0.01%), carboxymethyl cellulose gel or emulsifiable oils (Graxol® or Assist®) and exposed to heat for 6 or 8 h. In addition, conidia of IP 46 suspended in either pure mineral or canola oil and exposed to heat for 48 h had moderate viability, 57% or 41%, respectively. Unstable oil-in-water emulsions showed a higher percentage of conidia incorporated into oil micellae, while the stable emulsions had higher percentage of conidia outside the oil micellae. The thermotolerance of conidia formulated in stable emulsions, however, did not differ from that of conidia formulated in unstable emulsions. The present study highlights possibilities to alleviate the deleterious effects of heat stress towards Metarhizium spp. conidia applied for controlling arthropod pests and vectors through oil-based formulations.Fil: Santos Da Paixao, Flavia Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina. Universidade Federal de Goiás; BrasilFil: Muniz, E.R.. Universidade Federal de Goiás; BrasilFil: Barreto, L. P.. Universidade Federal de Goiás; BrasilFil: Bernardo, C. C.. Universidade Federal de Goiás; BrasilFil: Mascarin, G. M.. Universidade Federal de Goiás; BrasilFil: Luz, C.. Universidade Federal de Goiás; BrasilFil: Fernandes, Éverton. Universidade Federal de Goiás; BrasilTaylor & Francis Ltd2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48987Santos Da Paixao, Flavia Regina; Muniz, E.R.; Barreto, L. P.; Bernardo, C. C.; Mascarin, G. M.; et al.; Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii; Taylor & Francis Ltd; Biocontrol Science and Technology; 27; 3; 3-2017; 324-3370958-3157CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/09583157.2017.1281380info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09583157.2017.1281380info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:10:33Zoai:ri.conicet.gov.ar:11336/48987instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:10:34.069CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii
title Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii
spellingShingle Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii
Santos Da Paixao, Flavia Regina
Adjuvants
Conidial Thermotolerance
Entomopathogenic Fungi
Oil-In-Water Emulsion
title_short Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii
title_full Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii
title_fullStr Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii
title_full_unstemmed Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii
title_sort Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii
dc.creator.none.fl_str_mv Santos Da Paixao, Flavia Regina
Muniz, E.R.
Barreto, L. P.
Bernardo, C. C.
Mascarin, G. M.
Luz, C.
Fernandes, Éverton
author Santos Da Paixao, Flavia Regina
author_facet Santos Da Paixao, Flavia Regina
Muniz, E.R.
Barreto, L. P.
Bernardo, C. C.
Mascarin, G. M.
Luz, C.
Fernandes, Éverton
author_role author
author2 Muniz, E.R.
Barreto, L. P.
Bernardo, C. C.
Mascarin, G. M.
Luz, C.
Fernandes, Éverton
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Adjuvants
Conidial Thermotolerance
Entomopathogenic Fungi
Oil-In-Water Emulsion
topic Adjuvants
Conidial Thermotolerance
Entomopathogenic Fungi
Oil-In-Water Emulsion
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The thermotolerance of oil-based conidial formulations of Metarhizium anisopliae s.l. (IP 46) and Metarhizium robertsii (ARSEF 2575) were investigated. Conidia of IP 46 or ARSEF 2575 were suspended in different adjuvants and exposed to 45 ± 0.2°C for 4, 6, 8 or 24 h; their viability was then assessed after 48 h incubation at 27 ± 1°C. Conidia heated in pure mineral or vegetable oil exhibited mean relative viability exceeding 70% after 8 h of heat exposure, whereas low germination (≤20%) was observed when conidia were heated in water (Tween 80® 0.01%), carboxymethyl cellulose gel or emulsifiable oils (Graxol® or Assist®) and exposed to heat for 6 or 8 h. In addition, conidia of IP 46 suspended in either pure mineral or canola oil and exposed to heat for 48 h had moderate viability, 57% or 41%, respectively. Unstable oil-in-water emulsions showed a higher percentage of conidia incorporated into oil micellae, while the stable emulsions had higher percentage of conidia outside the oil micellae. The thermotolerance of conidia formulated in stable emulsions, however, did not differ from that of conidia formulated in unstable emulsions. The present study highlights possibilities to alleviate the deleterious effects of heat stress towards Metarhizium spp. conidia applied for controlling arthropod pests and vectors through oil-based formulations.
Fil: Santos Da Paixao, Flavia Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina. Universidade Federal de Goiás; Brasil
Fil: Muniz, E.R.. Universidade Federal de Goiás; Brasil
Fil: Barreto, L. P.. Universidade Federal de Goiás; Brasil
Fil: Bernardo, C. C.. Universidade Federal de Goiás; Brasil
Fil: Mascarin, G. M.. Universidade Federal de Goiás; Brasil
Fil: Luz, C.. Universidade Federal de Goiás; Brasil
Fil: Fernandes, Éverton. Universidade Federal de Goiás; Brasil
description The thermotolerance of oil-based conidial formulations of Metarhizium anisopliae s.l. (IP 46) and Metarhizium robertsii (ARSEF 2575) were investigated. Conidia of IP 46 or ARSEF 2575 were suspended in different adjuvants and exposed to 45 ± 0.2°C for 4, 6, 8 or 24 h; their viability was then assessed after 48 h incubation at 27 ± 1°C. Conidia heated in pure mineral or vegetable oil exhibited mean relative viability exceeding 70% after 8 h of heat exposure, whereas low germination (≤20%) was observed when conidia were heated in water (Tween 80® 0.01%), carboxymethyl cellulose gel or emulsifiable oils (Graxol® or Assist®) and exposed to heat for 6 or 8 h. In addition, conidia of IP 46 suspended in either pure mineral or canola oil and exposed to heat for 48 h had moderate viability, 57% or 41%, respectively. Unstable oil-in-water emulsions showed a higher percentage of conidia incorporated into oil micellae, while the stable emulsions had higher percentage of conidia outside the oil micellae. The thermotolerance of conidia formulated in stable emulsions, however, did not differ from that of conidia formulated in unstable emulsions. The present study highlights possibilities to alleviate the deleterious effects of heat stress towards Metarhizium spp. conidia applied for controlling arthropod pests and vectors through oil-based formulations.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48987
Santos Da Paixao, Flavia Regina; Muniz, E.R.; Barreto, L. P.; Bernardo, C. C.; Mascarin, G. M.; et al.; Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii; Taylor & Francis Ltd; Biocontrol Science and Technology; 27; 3; 3-2017; 324-337
0958-3157
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48987
identifier_str_mv Santos Da Paixao, Flavia Regina; Muniz, E.R.; Barreto, L. P.; Bernardo, C. C.; Mascarin, G. M.; et al.; Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii; Taylor & Francis Ltd; Biocontrol Science and Technology; 27; 3; 3-2017; 324-337
0958-3157
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/09583157.2017.1281380
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09583157.2017.1281380
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.229304

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