Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
- Autores
- Rengifo Herrera, Julián Andrés; Castaño, Olga L.; Sanabria, Irma Janeth
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
Centro de Investigación y Desarrollo en Ciencias Aplicadas - Materia
-
Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/104624
Ver los metadatos del registro completo
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Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiationRengifo Herrera, Julián AndrésCastaño, Olga L.Sanabria, Irma JanethQuímicaEcologíaPhoto-fenton disinfectionSalmonella typhimurium inactivationVBNC stateViability and cultivabilityCulturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.Centro de Investigación y Desarrollo en Ciencias Aplicadas2013-08-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf21-27http://sedici.unlp.edu.ar/handle/10915/104624enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/4932info:eu-repo/semantics/altIdentifier/issn/1945-3094info:eu-repo/semantics/altIdentifier/doi/10.4236/jwarp.2013.58A003info:eu-repo/semantics/altIdentifier/hdl/11336/4932info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:42Zoai:sedici.unlp.edu.ar:10915/104624Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:43.272SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation |
title |
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation |
spellingShingle |
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation Rengifo Herrera, Julián Andrés Química Ecología Photo-fenton disinfection Salmonella typhimurium inactivation VBNC state Viability and cultivability |
title_short |
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation |
title_full |
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation |
title_fullStr |
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation |
title_full_unstemmed |
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation |
title_sort |
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation |
dc.creator.none.fl_str_mv |
Rengifo Herrera, Julián Andrés Castaño, Olga L. Sanabria, Irma Janeth |
author |
Rengifo Herrera, Julián Andrés |
author_facet |
Rengifo Herrera, Julián Andrés Castaño, Olga L. Sanabria, Irma Janeth |
author_role |
author |
author2 |
Castaño, Olga L. Sanabria, Irma Janeth |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Química Ecología Photo-fenton disinfection Salmonella typhimurium inactivation VBNC state Viability and cultivability |
topic |
Química Ecología Photo-fenton disinfection Salmonella typhimurium inactivation VBNC state Viability and cultivability |
dc.description.none.fl_txt_mv |
Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state. Centro de Investigación y Desarrollo en Ciencias Aplicadas |
description |
Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/104624 |
url |
http://sedici.unlp.edu.ar/handle/10915/104624 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/4932 info:eu-repo/semantics/altIdentifier/issn/1945-3094 info:eu-repo/semantics/altIdentifier/doi/10.4236/jwarp.2013.58A003 info:eu-repo/semantics/altIdentifier/hdl/11336/4932 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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