Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation

Autores
Rengifo Herrera, Julián Andrés; Castaño, Olga L.; Sanabria, Irma Janeth
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
Centro de Investigación y Desarrollo en Ciencias Aplicadas
Materia
Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104624

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spelling Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiationRengifo Herrera, Julián AndrésCastaño, Olga L.Sanabria, Irma JanethQuímicaEcologíaPhoto-fenton disinfectionSalmonella typhimurium inactivationVBNC stateViability and cultivabilityCulturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.Centro de Investigación y Desarrollo en Ciencias Aplicadas2013-08-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf21-27http://sedici.unlp.edu.ar/handle/10915/104624enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/4932info:eu-repo/semantics/altIdentifier/issn/1945-3094info:eu-repo/semantics/altIdentifier/doi/10.4236/jwarp.2013.58A003info:eu-repo/semantics/altIdentifier/hdl/11336/4932info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:42Zoai:sedici.unlp.edu.ar:10915/104624Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:43.272SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
spellingShingle Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
Rengifo Herrera, Julián Andrés
Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability
title_short Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_full Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_fullStr Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_full_unstemmed Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_sort Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
dc.creator.none.fl_str_mv Rengifo Herrera, Julián Andrés
Castaño, Olga L.
Sanabria, Irma Janeth
author Rengifo Herrera, Julián Andrés
author_facet Rengifo Herrera, Julián Andrés
Castaño, Olga L.
Sanabria, Irma Janeth
author_role author
author2 Castaño, Olga L.
Sanabria, Irma Janeth
author2_role author
author
dc.subject.none.fl_str_mv Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability
topic Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability
dc.description.none.fl_txt_mv Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
Centro de Investigación y Desarrollo en Ciencias Aplicadas
description Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104624
url http://sedici.unlp.edu.ar/handle/10915/104624
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/4932
info:eu-repo/semantics/altIdentifier/issn/1945-3094
info:eu-repo/semantics/altIdentifier/doi/10.4236/jwarp.2013.58A003
info:eu-repo/semantics/altIdentifier/hdl/11336/4932
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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