Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation

Autores
Rengifo Herrera, Julian Andres; Castaño, Olga L.; Sanabria, Irma Janeth
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
Fil: Rengifo Herrera, Julian Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; Argentina
Fil: Castaño, Olga L.. Universidad del Valle. Grupo de Investigación en Procesos de Oxidación Avanzada para Química y Tratamientos Biológicos; Colombia
Fil: Sanabria, Irma Janeth. Universidad del Valle. Grupo de Investigación en Procesos de Oxidación Avanzada para Química y Tratamientos Biológicos; Colombia
Materia
Photo-Fenton disinfection
Salmonella typhimurium Inactivation
VBNC state
Viability and cultivability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4932
Acceder
id CONICETDig_3fbc1421ba314617ae08457374e8e574
oai_identifier_str oai:ri.conicet.gov.ar:11336/4932
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiationRengifo Herrera, Julian AndresCastaño, Olga L.Sanabria, Irma JanethPhoto-Fenton disinfectionSalmonella typhimurium InactivationVBNC stateViability and cultivabilityhttps://purl.org/becyt/ford/2.7https://purl.org/becyt/ford/2Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.Fil: Rengifo Herrera, Julian Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; ArgentinaFil: Castaño, Olga L.. Universidad del Valle. Grupo de Investigación en Procesos de Oxidación Avanzada para Química y Tratamientos Biológicos; ColombiaFil: Sanabria, Irma Janeth. Universidad del Valle. Grupo de Investigación en Procesos de Oxidación Avanzada para Química y Tratamientos Biológicos; ColombiaScientific Research Publishing2013-08-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4932Rengifo Herrera, Julian Andres; Castaño, Olga L.; Sanabria, Irma Janeth; Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation; Scientific Research Publishing; Journal of Water Resource and Protection; 5; 8A; 9-8-2013; 21-271945-3094enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.4236/jwarp.2013.58A003info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/PaperInformation.aspx?PaperID=35674info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:52Zoai:ri.conicet.gov.ar:11336/4932instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:53.199CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
spellingShingle Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
Rengifo Herrera, Julian Andres
Photo-Fenton disinfection
Salmonella typhimurium Inactivation
VBNC state
Viability and cultivability
title_short Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_full Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_fullStr Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_full_unstemmed Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_sort Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
dc.creator.none.fl_str_mv Rengifo Herrera, Julian Andres
Castaño, Olga L.
Sanabria, Irma Janeth
author Rengifo Herrera, Julian Andres
author_facet Rengifo Herrera, Julian Andres
Castaño, Olga L.
Sanabria, Irma Janeth
author_role author
author2 Castaño, Olga L.
Sanabria, Irma Janeth
author2_role author
author
dc.subject.none.fl_str_mv Photo-Fenton disinfection
Salmonella typhimurium Inactivation
VBNC state
Viability and cultivability
topic Photo-Fenton disinfection
Salmonella typhimurium Inactivation
VBNC state
Viability and cultivability
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.7
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
Fil: Rengifo Herrera, Julian Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; Argentina
Fil: Castaño, Olga L.. Universidad del Valle. Grupo de Investigación en Procesos de Oxidación Avanzada para Química y Tratamientos Biológicos; Colombia
Fil: Sanabria, Irma Janeth. Universidad del Valle. Grupo de Investigación en Procesos de Oxidación Avanzada para Química y Tratamientos Biológicos; Colombia
description Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4932
Rengifo Herrera, Julian Andres; Castaño, Olga L.; Sanabria, Irma Janeth; Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation; Scientific Research Publishing; Journal of Water Resource and Protection; 5; 8A; 9-8-2013; 21-27
1945-3094
url http://hdl.handle.net/11336/4932
identifier_str_mv Rengifo Herrera, Julian Andres; Castaño, Olga L.; Sanabria, Irma Janeth; Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation; Scientific Research Publishing; Journal of Water Resource and Protection; 5; 8A; 9-8-2013; 21-27
1945-3094
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.4236/jwarp.2013.58A003
info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/PaperInformation.aspx?PaperID=35674
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research Publishing
publisher.none.fl_str_mv Scientific Research Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613349420040192
score 13.070432

Publicaciones similares