Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation

Autores
Cattelan, Natalia; Villalba, María Inés; Parisi, Gustavo Daniel; Arnal, Laura; Serra, Diego Omar; Aguilar, Orlando Mario; Yantorno, Osvaldo Miguel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular
Materia
Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/96595

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network_name_str SEDICI (UNLP)
spelling Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formationCattelan, NataliaVillalba, María InésParisi, Gustavo DanielArnal, LauraSerra, Diego OmarAguilar, Orlando MarioYantorno, Osvaldo MiguelBiologíaCiencias NaturalesBiofilmBordetella bronchisepticaOmpqPertussisBordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesInstituto de Biotecnologia y Biologia Molecular2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf351-363http://sedici.unlp.edu.ar/handle/10915/96595enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/66425info:eu-repo/semantics/altIdentifier/url/http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000224info:eu-repo/semantics/altIdentifier/issn/1350-0872info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000224info:eu-repo/semantics/altIdentifier/hdl/11336/66425info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/ar/Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:20Zoai:sedici.unlp.edu.ar:10915/96595Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:21.033SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
spellingShingle Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
Cattelan, Natalia
Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis
title_short Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_full Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_fullStr Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_full_unstemmed Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_sort Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
dc.creator.none.fl_str_mv Cattelan, Natalia
Villalba, María Inés
Parisi, Gustavo Daniel
Arnal, Laura
Serra, Diego Omar
Aguilar, Orlando Mario
Yantorno, Osvaldo Miguel
author Cattelan, Natalia
author_facet Cattelan, Natalia
Villalba, María Inés
Parisi, Gustavo Daniel
Arnal, Laura
Serra, Diego Omar
Aguilar, Orlando Mario
Yantorno, Osvaldo Miguel
author_role author
author2 Villalba, María Inés
Parisi, Gustavo Daniel
Arnal, Laura
Serra, Diego Omar
Aguilar, Orlando Mario
Yantorno, Osvaldo Miguel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis
topic Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis
dc.description.none.fl_txt_mv Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular
description Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/96595
url http://sedici.unlp.edu.ar/handle/10915/96595
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/66425
info:eu-repo/semantics/altIdentifier/url/http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000224
info:eu-repo/semantics/altIdentifier/issn/1350-0872
info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000224
info:eu-repo/semantics/altIdentifier/hdl/11336/66425
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5)
dc.format.none.fl_str_mv application/pdf
351-363
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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