Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
- Autores
- Cattelan, Natalia; Villalba, María Inés; Parisi, Gustavo Daniel; Arnal, Laura; Serra, Diego Omar; Aguilar, Orlando Mario; Yantorno, Osvaldo Miguel
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular - Materia
-
Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/96595
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Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formationCattelan, NataliaVillalba, María InésParisi, Gustavo DanielArnal, LauraSerra, Diego OmarAguilar, Orlando MarioYantorno, Osvaldo MiguelBiologíaCiencias NaturalesBiofilmBordetella bronchisepticaOmpqPertussisBordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesInstituto de Biotecnologia y Biologia Molecular2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf351-363http://sedici.unlp.edu.ar/handle/10915/96595enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/66425info:eu-repo/semantics/altIdentifier/url/http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000224info:eu-repo/semantics/altIdentifier/issn/1350-0872info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000224info:eu-repo/semantics/altIdentifier/hdl/11336/66425info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/ar/Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:20Zoai:sedici.unlp.edu.ar:10915/96595Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:21.033SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation |
title |
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation |
spellingShingle |
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation Cattelan, Natalia Biología Ciencias Naturales Biofilm Bordetella bronchiseptica Ompq Pertussis |
title_short |
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation |
title_full |
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation |
title_fullStr |
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation |
title_full_unstemmed |
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation |
title_sort |
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation |
dc.creator.none.fl_str_mv |
Cattelan, Natalia Villalba, María Inés Parisi, Gustavo Daniel Arnal, Laura Serra, Diego Omar Aguilar, Orlando Mario Yantorno, Osvaldo Miguel |
author |
Cattelan, Natalia |
author_facet |
Cattelan, Natalia Villalba, María Inés Parisi, Gustavo Daniel Arnal, Laura Serra, Diego Omar Aguilar, Orlando Mario Yantorno, Osvaldo Miguel |
author_role |
author |
author2 |
Villalba, María Inés Parisi, Gustavo Daniel Arnal, Laura Serra, Diego Omar Aguilar, Orlando Mario Yantorno, Osvaldo Miguel |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Biología Ciencias Naturales Biofilm Bordetella bronchiseptica Ompq Pertussis |
topic |
Biología Ciencias Naturales Biofilm Bordetella bronchiseptica Ompq Pertussis |
dc.description.none.fl_txt_mv |
Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition. Centro de Investigación y Desarrollo en Fermentaciones Industriales Instituto de Biotecnologia y Biologia Molecular |
description |
Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/96595 |
url |
http://sedici.unlp.edu.ar/handle/10915/96595 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
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dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5) |
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