Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>
- Autores
- Ambrosis, Nicolás Martín; Boyd, Chelsea D.; O'Toole, George A.; Fernández, Julieta; Sisti, Federico
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Biofilm formation is important for infection by many pathogens. Bordetella bronchiseptica causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formation in B. bronchiseptica. In the present work, based on their previously reported function in Pseudomonas fluorescens, we identified three genes in the B. bronchiseptica genome likely involved in c-di-GMP-dependent biofilm formation: brtA, lapD and lapG. Genetic analysis confirmed a role for BrtA, LapD and LapG in biofilm formation using microtiter plate assays, as well as scanning electron and fluorescent microscopy to analyze the phenotypes of mutants lacking these proteins. In vitro and in vivo studies showed that the protease LapG of B. bronchiseptica cleaves the N-terminal domain of BrtA, as well as the LapA protein of P. fluorescens, indicating functional conservation between these species. Furthermore, while BrtA and LapG appear to have little or no impact on colonization in a mouse model of infection, a B. bronchiseptica strain lacking the LapG protease has a significantly higher rate of inducing a severe disease outcome compared to the wild type. These findings support a role for c-di-GMP acting through BrtA/LapD/LapG to modulate biofilm formation, as well as impact pathogenesis, by B. bronchiseptica.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular - Materia
-
Ciencias Exactas
Bordetella bronchiseptica
respiratory tract infections - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/86497
Ver los metadatos del registro completo
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Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>Ambrosis, Nicolás MartínBoyd, Chelsea D.O'Toole, George A.Fernández, JulietaSisti, FedericoCiencias ExactasBordetella bronchisepticarespiratory tract infectionsBiofilm formation is important for infection by many pathogens. <i>Bordetella bronchiseptica</i> causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formation in <i>B. bronchiseptica</i>. In the present work, based on their previously reported function in <i>Pseudomonas fluorescens</i>, we identified three genes in the <i>B. bronchiseptica</i> genome likely involved in c-di-GMP-dependent biofilm formation: brtA, lapD and lapG. Genetic analysis confirmed a role for BrtA, LapD and LapG in biofilm formation using microtiter plate assays, as well as scanning electron and fluorescent microscopy to analyze the phenotypes of mutants lacking these proteins. <i>In vitro</i> and <i>in vivo</i> studies showed that the protease LapG of <i>B. bronchiseptica</i> cleaves the N-terminal domain of BrtA, as well as the LapA protein of <i>P. fluorescens</i>, indicating functional conservation between these species. Furthermore, while BrtA and LapG appear to have little or no impact on colonization in a mouse model of infection, a <i>B. bronchiseptica</i> strain lacking the LapG protease has a significantly higher rate of inducing a severe disease outcome compared to the wild type. These findings support a role for c-di-GMP acting through BrtA/LapD/LapG to modulate biofilm formation, as well as impact pathogenesis, by <i>B. bronchiseptica</i>.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia Molecular2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86497enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0158752info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:45Zoai:sedici.unlp.edu.ar:10915/86497Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:45.444SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> |
title |
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> |
spellingShingle |
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> Ambrosis, Nicolás Martín Ciencias Exactas Bordetella bronchiseptica respiratory tract infections |
title_short |
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> |
title_full |
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> |
title_fullStr |
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> |
title_full_unstemmed |
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> |
title_sort |
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> |
dc.creator.none.fl_str_mv |
Ambrosis, Nicolás Martín Boyd, Chelsea D. O'Toole, George A. Fernández, Julieta Sisti, Federico |
author |
Ambrosis, Nicolás Martín |
author_facet |
Ambrosis, Nicolás Martín Boyd, Chelsea D. O'Toole, George A. Fernández, Julieta Sisti, Federico |
author_role |
author |
author2 |
Boyd, Chelsea D. O'Toole, George A. Fernández, Julieta Sisti, Federico |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Bordetella bronchiseptica respiratory tract infections |
topic |
Ciencias Exactas Bordetella bronchiseptica respiratory tract infections |
dc.description.none.fl_txt_mv |
Biofilm formation is important for infection by many pathogens. <i>Bordetella bronchiseptica</i> causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formation in <i>B. bronchiseptica</i>. In the present work, based on their previously reported function in <i>Pseudomonas fluorescens</i>, we identified three genes in the <i>B. bronchiseptica</i> genome likely involved in c-di-GMP-dependent biofilm formation: brtA, lapD and lapG. Genetic analysis confirmed a role for BrtA, LapD and LapG in biofilm formation using microtiter plate assays, as well as scanning electron and fluorescent microscopy to analyze the phenotypes of mutants lacking these proteins. <i>In vitro</i> and <i>in vivo</i> studies showed that the protease LapG of <i>B. bronchiseptica</i> cleaves the N-terminal domain of BrtA, as well as the LapA protein of <i>P. fluorescens</i>, indicating functional conservation between these species. Furthermore, while BrtA and LapG appear to have little or no impact on colonization in a mouse model of infection, a <i>B. bronchiseptica</i> strain lacking the LapG protease has a significantly higher rate of inducing a severe disease outcome compared to the wild type. These findings support a role for c-di-GMP acting through BrtA/LapD/LapG to modulate biofilm formation, as well as impact pathogenesis, by <i>B. bronchiseptica</i>. Facultad de Ciencias Exactas Instituto de Biotecnologia y Biologia Molecular |
description |
Biofilm formation is important for infection by many pathogens. <i>Bordetella bronchiseptica</i> causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formation in <i>B. bronchiseptica</i>. In the present work, based on their previously reported function in <i>Pseudomonas fluorescens</i>, we identified three genes in the <i>B. bronchiseptica</i> genome likely involved in c-di-GMP-dependent biofilm formation: brtA, lapD and lapG. Genetic analysis confirmed a role for BrtA, LapD and LapG in biofilm formation using microtiter plate assays, as well as scanning electron and fluorescent microscopy to analyze the phenotypes of mutants lacking these proteins. <i>In vitro</i> and <i>in vivo</i> studies showed that the protease LapG of <i>B. bronchiseptica</i> cleaves the N-terminal domain of BrtA, as well as the LapA protein of <i>P. fluorescens</i>, indicating functional conservation between these species. Furthermore, while BrtA and LapG appear to have little or no impact on colonization in a mouse model of infection, a <i>B. bronchiseptica</i> strain lacking the LapG protease has a significantly higher rate of inducing a severe disease outcome compared to the wild type. These findings support a role for c-di-GMP acting through BrtA/LapD/LapG to modulate biofilm formation, as well as impact pathogenesis, by <i>B. bronchiseptica</i>. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 |
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