Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
- Autores
- Gambaro, Sabrina Eliana; Zubiría, María Guillermina; Giordano, Alejandra; Castro, Patricia F.; Garraza, Carolina; Harnichar, Alejandro Ezequiel; Alzamendi, Ana; Spinedi, Eduardo Julio; Giovambattista, Andrés
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.
Instituto Multidisciplinario de Biología Celular
Centro de Endocrinología Experimental y Aplicada - Materia
-
Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/167624
Ver los metadatos del registro completo
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Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated ConditionsGambaro, Sabrina ElianaZubiría, María GuillerminaGiordano, AlejandraCastro, Patricia F.Garraza, CarolinaHarnichar, Alejandro EzequielAlzamendi, AnaSpinedi, Eduardo JulioGiovambattista, AndrésBiologíaSpexinthermogenesisbeige adipocyteswhite adipose tissueUCP1Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.Instituto Multidisciplinario de Biología CelularCentro de Endocrinología Experimental y Aplicada2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/167624enginfo:eu-repo/semantics/altIdentifier/issn/1422-0067info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25031767info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:44:40Zoai:sedici.unlp.edu.ar:10915/167624Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:44:40.781SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions |
title |
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions |
spellingShingle |
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions Gambaro, Sabrina Eliana Biología Spexin thermogenesis beige adipocytes white adipose tissue UCP1 |
title_short |
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions |
title_full |
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions |
title_fullStr |
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions |
title_full_unstemmed |
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions |
title_sort |
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions |
dc.creator.none.fl_str_mv |
Gambaro, Sabrina Eliana Zubiría, María Guillermina Giordano, Alejandra Castro, Patricia F. Garraza, Carolina Harnichar, Alejandro Ezequiel Alzamendi, Ana Spinedi, Eduardo Julio Giovambattista, Andrés |
author |
Gambaro, Sabrina Eliana |
author_facet |
Gambaro, Sabrina Eliana Zubiría, María Guillermina Giordano, Alejandra Castro, Patricia F. Garraza, Carolina Harnichar, Alejandro Ezequiel Alzamendi, Ana Spinedi, Eduardo Julio Giovambattista, Andrés |
author_role |
author |
author2 |
Zubiría, María Guillermina Giordano, Alejandra Castro, Patricia F. Garraza, Carolina Harnichar, Alejandro Ezequiel Alzamendi, Ana Spinedi, Eduardo Julio Giovambattista, Andrés |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Biología Spexin thermogenesis beige adipocytes white adipose tissue UCP1 |
topic |
Biología Spexin thermogenesis beige adipocytes white adipose tissue UCP1 |
dc.description.none.fl_txt_mv |
Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation. Instituto Multidisciplinario de Biología Celular Centro de Endocrinología Experimental y Aplicada |
description |
Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024 |
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