Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions

Autores
Gambaro, Sabrina Eliana; Zubiría, María Guillermina; Giordano, Alejandra; Castro, Patricia F.; Garraza, Carolina; Harnichar, Alejandro Ezequiel; Alzamendi, Ana; Spinedi, Eduardo Julio; Giovambattista, Andrés
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.
Instituto Multidisciplinario de Biología Celular
Centro de Endocrinología Experimental y Aplicada
Materia
Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/167624
Acceder
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network_name_str SEDICI (UNLP)
spelling Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated ConditionsGambaro, Sabrina ElianaZubiría, María GuillerminaGiordano, AlejandraCastro, Patricia F.Garraza, CarolinaHarnichar, Alejandro EzequielAlzamendi, AnaSpinedi, Eduardo JulioGiovambattista, AndrésBiologíaSpexinthermogenesisbeige adipocyteswhite adipose tissueUCP1Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.Instituto Multidisciplinario de Biología CelularCentro de Endocrinología Experimental y Aplicada2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/167624enginfo:eu-repo/semantics/altIdentifier/issn/1422-0067info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25031767info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:25:33Zoai:sedici.unlp.edu.ar:10915/167624Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:25:33.683SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
spellingShingle Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
Gambaro, Sabrina Eliana
Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1
title_short Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_full Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_fullStr Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_full_unstemmed Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_sort Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
dc.creator.none.fl_str_mv Gambaro, Sabrina Eliana
Zubiría, María Guillermina
Giordano, Alejandra
Castro, Patricia F.
Garraza, Carolina
Harnichar, Alejandro Ezequiel
Alzamendi, Ana
Spinedi, Eduardo Julio
Giovambattista, Andrés
author Gambaro, Sabrina Eliana
author_facet Gambaro, Sabrina Eliana
Zubiría, María Guillermina
Giordano, Alejandra
Castro, Patricia F.
Garraza, Carolina
Harnichar, Alejandro Ezequiel
Alzamendi, Ana
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_role author
author2 Zubiría, María Guillermina
Giordano, Alejandra
Castro, Patricia F.
Garraza, Carolina
Harnichar, Alejandro Ezequiel
Alzamendi, Ana
Spinedi, Eduardo Julio
Giovambattista, Andrés
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1
topic Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1
dc.description.none.fl_txt_mv Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.
Instituto Multidisciplinario de Biología Celular
Centro de Endocrinología Experimental y Aplicada
description Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/167624
url http://sedici.unlp.edu.ar/handle/10915/167624
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1422-0067
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25031767
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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