Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina

Autores
Yanniccari, Marcos; Gómez Lobato, María Eugenia; Istilart, Carolina; Natalucci, Claudia Luisa; Giménez, Daniel Oscar; Castro, Ana María
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.
Instituto de Fisiología Vegetal
Facultad de Ciencias Agrarias y Forestales
Centro de Investigación de Proteínas Vegetales
Materia
Ciencias Agrarias
Ciencias Exactas
EPSPS activity
EPSPS overexpression
Glyphosate resistance
Perennial ryegrass
Shikimate assay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87593
Acceder
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network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from ArgentinaYanniccari, MarcosGómez Lobato, María EugeniaIstilart, CarolinaNatalucci, Claudia LuisaGiménez, Daniel OscarCastro, Ana MaríaCiencias AgrariasCiencias ExactasEPSPS activityEPSPS overexpressionGlyphosate resistancePerennial ryegrassShikimate assayIn Argentina, glyphosate resistance was reported in a <i>Lolium perenne</i> population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated <i>in vitro</i> showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.Instituto de Fisiología VegetalFacultad de Ciencias Agrarias y ForestalesCentro de Investigación de Proteínas Vegetales2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87593enginfo:eu-repo/semantics/altIdentifier/issn/2296-701Xinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fevo.2017.00123info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:10Zoai:sedici.unlp.edu.ar:10915/87593Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:10.304SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
spellingShingle Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
Yanniccari, Marcos
Ciencias Agrarias
Ciencias Exactas
EPSPS activity
EPSPS overexpression
Glyphosate resistance
Perennial ryegrass
Shikimate assay
title_short Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_full Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_fullStr Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_full_unstemmed Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_sort Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
dc.creator.none.fl_str_mv Yanniccari, Marcos
Gómez Lobato, María Eugenia
Istilart, Carolina
Natalucci, Claudia Luisa
Giménez, Daniel Oscar
Castro, Ana María
author Yanniccari, Marcos
author_facet Yanniccari, Marcos
Gómez Lobato, María Eugenia
Istilart, Carolina
Natalucci, Claudia Luisa
Giménez, Daniel Oscar
Castro, Ana María
author_role author
author2 Gómez Lobato, María Eugenia
Istilart, Carolina
Natalucci, Claudia Luisa
Giménez, Daniel Oscar
Castro, Ana María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Ciencias Exactas
EPSPS activity
EPSPS overexpression
Glyphosate resistance
Perennial ryegrass
Shikimate assay
topic Ciencias Agrarias
Ciencias Exactas
EPSPS activity
EPSPS overexpression
Glyphosate resistance
Perennial ryegrass
Shikimate assay
dc.description.none.fl_txt_mv In Argentina, glyphosate resistance was reported in a <i>Lolium perenne</i> population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated <i>in vitro</i> showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.
Instituto de Fisiología Vegetal
Facultad de Ciencias Agrarias y Forestales
Centro de Investigación de Proteínas Vegetales
description In Argentina, glyphosate resistance was reported in a <i>Lolium perenne</i> population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated <i>in vitro</i> showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/87593
url http://sedici.unlp.edu.ar/handle/10915/87593
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2296-701X
info:eu-repo/semantics/altIdentifier/doi/10.3389/fevo.2017.00123
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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