Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina

Autores
Castillo, Nadia; Cordo, Cristina Alicia; Simón, María Rosa
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)₄; (AAC)₇; (ATC)₇; (AC)₉; (AAG)₇; (AG)₉; (AGC)₅; (CAG)₅, (GTG)₅ and (GACAC)₃. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)₇ and (GTG)₅, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina.
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología
Materia
Ciencias Agrarias
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131456
Acceder
id SEDICI_22b1ca5522e1e85d80f3ed06b12d13b9
oai_identifier_str oai:sedici.unlp.edu.ar:10915/131456
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in ArgentinaCastillo, NadiaCordo, Cristina AliciaSimón, María RosaCiencias AgrariasDendrogramGenetic diversityHaplotypesISSRPopulationThe genetic structure and diversity of <i>Mycosphaerella graminicola</i> population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (<i>Triticum aestivum</i>) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)₄; (AAC)₇; (ATC)₇; (AC)₉; (AAG)₇; (AG)₉; (AGC)₅; (CAG)₅, (GTG)₅ and (GACAC)₃. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)₇ and (GTG)₅, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the <i>M. graminicola</i> population in Argentina.Facultad de Ciencias Agrarias y ForestalesCentro de Investigaciones en Fitopatología2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf379-389http://sedici.unlp.edu.ar/handle/10915/131456enginfo:eu-repo/semantics/altIdentifier/issn/0334-2123info:eu-repo/semantics/altIdentifier/issn/1876-7184info:eu-repo/semantics/altIdentifier/doi/10.1007/s12600-010-0110-zinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:17Zoai:sedici.unlp.edu.ar:10915/131456Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:18.169SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
spellingShingle Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
Castillo, Nadia
Ciencias Agrarias
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
title_short Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_full Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_fullStr Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_full_unstemmed Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_sort Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
dc.creator.none.fl_str_mv Castillo, Nadia
Cordo, Cristina Alicia
Simón, María Rosa
author Castillo, Nadia
author_facet Castillo, Nadia
Cordo, Cristina Alicia
Simón, María Rosa
author_role author
author2 Cordo, Cristina Alicia
Simón, María Rosa
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
topic Ciencias Agrarias
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
dc.description.none.fl_txt_mv The genetic structure and diversity of <i>Mycosphaerella graminicola</i> population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (<i>Triticum aestivum</i>) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)₄; (AAC)₇; (ATC)₇; (AC)₉; (AAG)₇; (AG)₉; (AGC)₅; (CAG)₅, (GTG)₅ and (GACAC)₃. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)₇ and (GTG)₅, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the <i>M. graminicola</i> population in Argentina.
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología
description The genetic structure and diversity of <i>Mycosphaerella graminicola</i> population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (<i>Triticum aestivum</i>) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)₄; (AAC)₇; (ATC)₇; (AC)₉; (AAG)₇; (AG)₉; (AGC)₅; (CAG)₅, (GTG)₅ and (GACAC)₃. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)₇ and (GTG)₅, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the <i>M. graminicola</i> population in Argentina.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131456
url http://sedici.unlp.edu.ar/handle/10915/131456
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0334-2123
info:eu-repo/semantics/altIdentifier/issn/1876-7184
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12600-010-0110-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
379-389
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616202719068160
score 13.070432

Publicaciones similares