Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina

Autores
Castillo, Nadia; Cordo, Cristina Alicia; Simón, María Rosa
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)4; (AAC)7; (ATC)7; (AC)9; (AAG)7; (AG)9; (AGC)5; (CAG)5, (GTG)5 and (GACAC)3. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)7 and (GTG)5, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina.
Materia
Ciencias Agrícolas
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/7259

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network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in ArgentinaCastillo, NadiaCordo, Cristina AliciaSimón, María RosaCiencias AgrícolasDendrogramGenetic diversityHaplotypesISSRPopulationThe genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)4; (AAC)7; (ATC)7; (AC)9; (AAG)7; (AG)9; (AGC)5; (CAG)5, (GTG)5 and (GACAC)3. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)7 and (GTG)5, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina.Springer International Publishing2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/7259enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:02Zoai:digital.cic.gba.gob.ar:11746/7259Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:02.773CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
title Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
spellingShingle Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
Castillo, Nadia
Ciencias Agrícolas
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
title_short Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
title_full Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
title_fullStr Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
title_full_unstemmed Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
title_sort Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
dc.creator.none.fl_str_mv Castillo, Nadia
Cordo, Cristina Alicia
Simón, María Rosa
author Castillo, Nadia
author_facet Castillo, Nadia
Cordo, Cristina Alicia
Simón, María Rosa
author_role author
author2 Cordo, Cristina Alicia
Simón, María Rosa
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Agrícolas
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
topic Ciencias Agrícolas
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
dc.description.none.fl_txt_mv The genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)4; (AAC)7; (ATC)7; (AC)9; (AAG)7; (AG)9; (AGC)5; (CAG)5, (GTG)5 and (GACAC)3. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)7 and (GTG)5, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina.
description The genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)4; (AAC)7; (ATC)7; (AC)9; (AAG)7; (AG)9; (AGC)5; (CAG)5, (GTG)5 and (GACAC)3. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)7 and (GTG)5, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/7259
url https://digital.cic.gba.gob.ar/handle/11746/7259
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer International Publishing
publisher.none.fl_str_mv Springer International Publishing
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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