Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina
- Autores
- Castillo, Nadia; Cordo, Cristina Alicia; Simón, María Rosa
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)4; (AAC)7; (ATC)7; (AC)9; (AAG)7; (AG)9; (AGC)5; (CAG)5, (GTG)5 and (GACAC)3. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)7 and (GTG)5, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina.
- Materia
-
Ciencias Agrícolas
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/7259
Ver los metadatos del registro completo
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oai:digital.cic.gba.gob.ar:11746/7259 |
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network_name_str |
CIC Digital (CICBA) |
spelling |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in ArgentinaCastillo, NadiaCordo, Cristina AliciaSimón, María RosaCiencias AgrícolasDendrogramGenetic diversityHaplotypesISSRPopulationThe genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)4; (AAC)7; (ATC)7; (AC)9; (AAG)7; (AG)9; (AGC)5; (CAG)5, (GTG)5 and (GACAC)3. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)7 and (GTG)5, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina.Springer International Publishing2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/7259enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:02Zoai:digital.cic.gba.gob.ar:11746/7259Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:02.773CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina |
title |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina |
spellingShingle |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina Castillo, Nadia Ciencias Agrícolas Dendrogram Genetic diversity Haplotypes ISSR Population |
title_short |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina |
title_full |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina |
title_fullStr |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina |
title_full_unstemmed |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina |
title_sort |
Molecular variability among isolates of Mycosphaerella graminicola, the causal agent of septoria tritici blotch, in Argentina |
dc.creator.none.fl_str_mv |
Castillo, Nadia Cordo, Cristina Alicia Simón, María Rosa |
author |
Castillo, Nadia |
author_facet |
Castillo, Nadia Cordo, Cristina Alicia Simón, María Rosa |
author_role |
author |
author2 |
Cordo, Cristina Alicia Simón, María Rosa |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Ciencias Agrícolas Dendrogram Genetic diversity Haplotypes ISSR Population |
topic |
Ciencias Agrícolas Dendrogram Genetic diversity Haplotypes ISSR Population |
dc.description.none.fl_txt_mv |
The genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)4; (AAC)7; (ATC)7; (AC)9; (AAG)7; (AG)9; (AGC)5; (CAG)5, (GTG)5 and (GACAC)3. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)7 and (GTG)5, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina. |
description |
The genetic structure and diversity of Mycosphaerella graminicola population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (Triticum aestivum) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)4; (AAC)7; (ATC)7; (AC)9; (AAG)7; (AG)9; (AGC)5; (CAG)5, (GTG)5 and (GACAC)3. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)7 and (GTG)5, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the M. graminicola population in Argentina. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/7259 |
url |
https://digital.cic.gba.gob.ar/handle/11746/7259 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer International Publishing |
publisher.none.fl_str_mv |
Springer International Publishing |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
_version_ |
1844618595489808384 |
score |
13.070432 |