Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cel...

Autores
Rodenak-Kladniew, Boris; Castro, María Agustina; Gambaro, Rocío Celeste; Girotti, Juan Roberto; Cisneros, José Sebastián; Viña, Sonia Zulma; Padula, Gisel; Crespo, Rosana; Castro, Guillermo Raúl; Gehring, Stephan; Chain, Cecilia Yamil; Islan, Germán Abel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus globulus, Origanum × paniculatum, Mentha × piperita, Mentha arvensis L., and Rosmarinus officinalis L. against human lung (A549) and colon (HCT-116) cancer cells. The cells were treated with increasing EO concentrations (0–500 µL/L) for 24 h, and cytotoxic activity was assessed. LaDEO and CnEO were the most potent EOs evaluated (IC50 range, 145–275 µL/L). The gas chromatography–mass spectrometry method was used to determine their composition. Considering EO limitations as therapeutic agents (poor water solubility, volatilization, and oxidation), we evaluated whether LaDEO and CnEO encapsulation into solid lipid nanoparticles (SLN/EO) enhanced their anticancer activity. Highly stable spherical SLN/LaDEO and SLN/CnEO SLN/EO were obtained, with a mean diameter of 140–150 nm, narrow size dispersion, and Z potential around −5mV. EO encapsulation strongly increased their anticancer activity, particularly in A549 cells exposed to SLN/CnEO (IC50 = 66 µL/L CnEO). The physicochemical characterization, biosafety, and anticancer mechanisms of SLN/CnEO were also evaluated in A549 cells. SLN/CnEO containing 97 ± 1% CnEO was highly stable for up to 6 months. An increased in vitro CnEO release from SLN at an acidic pH (endolysosomal compartment) was observed. SLN/CnEO proved to be safe against blood components and non-toxic for normal WI-38 cells at therapeutic concentrations. SLN/CnEO substantially enhanced A549 cell death and cell migration inhibition compared with free CnEO.
Instituto de Investigaciones Bioquímicas de La Plata
Instituto de Genética Veterinaria
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Química
Bioquímica
essential oils
solid lipid nanoparticles
cancer cells
biocompatibility
drug delivery
anticancer mechanisms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/160996
Acceder
id SEDICI_156f15b1ba32ef738dba0e4136980c86
oai_identifier_str oai:sedici.unlp.edu.ar:10915/160996
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cellsRodenak-Kladniew, BorisCastro, María AgustinaGambaro, Rocío CelesteGirotti, Juan RobertoCisneros, José SebastiánViña, Sonia ZulmaPadula, GiselCrespo, RosanaCastro, Guillermo RaúlGehring, StephanChain, Cecilia YamilIslan, Germán AbelQuímicaBioquímicaessential oilssolid lipid nanoparticlescancer cellsbiocompatibilitydrug deliveryanticancer mechanismsPlant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus globulus, Origanum × paniculatum, Mentha × piperita, Mentha arvensis L., and Rosmarinus officinalis L. against human lung (A549) and colon (HCT-116) cancer cells. The cells were treated with increasing EO concentrations (0–500 µL/L) for 24 h, and cytotoxic activity was assessed. LaDEO and CnEO were the most potent EOs evaluated (IC50 range, 145–275 µL/L). The gas chromatography–mass spectrometry method was used to determine their composition. Considering EO limitations as therapeutic agents (poor water solubility, volatilization, and oxidation), we evaluated whether LaDEO and CnEO encapsulation into solid lipid nanoparticles (SLN/EO) enhanced their anticancer activity. Highly stable spherical SLN/LaDEO and SLN/CnEO SLN/EO were obtained, with a mean diameter of 140–150 nm, narrow size dispersion, and Z potential around −5mV. EO encapsulation strongly increased their anticancer activity, particularly in A549 cells exposed to SLN/CnEO (IC50 = 66 µL/L CnEO). The physicochemical characterization, biosafety, and anticancer mechanisms of SLN/CnEO were also evaluated in A549 cells. SLN/CnEO containing 97 ± 1% CnEO was highly stable for up to 6 months. An increased in vitro CnEO release from SLN at an acidic pH (endolysosomal compartment) was observed. SLN/CnEO proved to be safe against blood components and non-toxic for normal WI-38 cells at therapeutic concentrations. SLN/CnEO substantially enhanced A549 cell death and cell migration inhibition compared with free CnEO.Instituto de Investigaciones Bioquímicas de La PlataInstituto de Genética VeterinariaInstituto de Investigaciones Fisicoquímicas Teóricas y AplicadasCentro de Investigación y Desarrollo en Criotecnología de AlimentosCentro de Investigación y Desarrollo en Fermentaciones Industriales2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/160996enginfo:eu-repo/semantics/altIdentifier/issn/1999-4923info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics15082045info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:42:11Zoai:sedici.unlp.edu.ar:10915/160996Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:42:12.055SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
spellingShingle Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
Rodenak-Kladniew, Boris
Química
Bioquímica
essential oils
solid lipid nanoparticles
cancer cells
biocompatibility
drug delivery
anticancer mechanisms
title_short Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_full Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_fullStr Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_full_unstemmed Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_sort Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
dc.creator.none.fl_str_mv Rodenak-Kladniew, Boris
Castro, María Agustina
Gambaro, Rocío Celeste
Girotti, Juan Roberto
Cisneros, José Sebastián
Viña, Sonia Zulma
Padula, Gisel
Crespo, Rosana
Castro, Guillermo Raúl
Gehring, Stephan
Chain, Cecilia Yamil
Islan, Germán Abel
author Rodenak-Kladniew, Boris
author_facet Rodenak-Kladniew, Boris
Castro, María Agustina
Gambaro, Rocío Celeste
Girotti, Juan Roberto
Cisneros, José Sebastián
Viña, Sonia Zulma
Padula, Gisel
Crespo, Rosana
Castro, Guillermo Raúl
Gehring, Stephan
Chain, Cecilia Yamil
Islan, Germán Abel
author_role author
author2 Castro, María Agustina
Gambaro, Rocío Celeste
Girotti, Juan Roberto
Cisneros, José Sebastián
Viña, Sonia Zulma
Padula, Gisel
Crespo, Rosana
Castro, Guillermo Raúl
Gehring, Stephan
Chain, Cecilia Yamil
Islan, Germán Abel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Química
Bioquímica
essential oils
solid lipid nanoparticles
cancer cells
biocompatibility
drug delivery
anticancer mechanisms
topic Química
Bioquímica
essential oils
solid lipid nanoparticles
cancer cells
biocompatibility
drug delivery
anticancer mechanisms
dc.description.none.fl_txt_mv Plant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus globulus, Origanum × paniculatum, Mentha × piperita, Mentha arvensis L., and Rosmarinus officinalis L. against human lung (A549) and colon (HCT-116) cancer cells. The cells were treated with increasing EO concentrations (0–500 µL/L) for 24 h, and cytotoxic activity was assessed. LaDEO and CnEO were the most potent EOs evaluated (IC50 range, 145–275 µL/L). The gas chromatography–mass spectrometry method was used to determine their composition. Considering EO limitations as therapeutic agents (poor water solubility, volatilization, and oxidation), we evaluated whether LaDEO and CnEO encapsulation into solid lipid nanoparticles (SLN/EO) enhanced their anticancer activity. Highly stable spherical SLN/LaDEO and SLN/CnEO SLN/EO were obtained, with a mean diameter of 140–150 nm, narrow size dispersion, and Z potential around −5mV. EO encapsulation strongly increased their anticancer activity, particularly in A549 cells exposed to SLN/CnEO (IC50 = 66 µL/L CnEO). The physicochemical characterization, biosafety, and anticancer mechanisms of SLN/CnEO were also evaluated in A549 cells. SLN/CnEO containing 97 ± 1% CnEO was highly stable for up to 6 months. An increased in vitro CnEO release from SLN at an acidic pH (endolysosomal compartment) was observed. SLN/CnEO proved to be safe against blood components and non-toxic for normal WI-38 cells at therapeutic concentrations. SLN/CnEO substantially enhanced A549 cell death and cell migration inhibition compared with free CnEO.
Instituto de Investigaciones Bioquímicas de La Plata
Instituto de Genética Veterinaria
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Plant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus globulus, Origanum × paniculatum, Mentha × piperita, Mentha arvensis L., and Rosmarinus officinalis L. against human lung (A549) and colon (HCT-116) cancer cells. The cells were treated with increasing EO concentrations (0–500 µL/L) for 24 h, and cytotoxic activity was assessed. LaDEO and CnEO were the most potent EOs evaluated (IC50 range, 145–275 µL/L). The gas chromatography–mass spectrometry method was used to determine their composition. Considering EO limitations as therapeutic agents (poor water solubility, volatilization, and oxidation), we evaluated whether LaDEO and CnEO encapsulation into solid lipid nanoparticles (SLN/EO) enhanced their anticancer activity. Highly stable spherical SLN/LaDEO and SLN/CnEO SLN/EO were obtained, with a mean diameter of 140–150 nm, narrow size dispersion, and Z potential around −5mV. EO encapsulation strongly increased their anticancer activity, particularly in A549 cells exposed to SLN/CnEO (IC50 = 66 µL/L CnEO). The physicochemical characterization, biosafety, and anticancer mechanisms of SLN/CnEO were also evaluated in A549 cells. SLN/CnEO containing 97 ± 1% CnEO was highly stable for up to 6 months. An increased in vitro CnEO release from SLN at an acidic pH (endolysosomal compartment) was observed. SLN/CnEO proved to be safe against blood components and non-toxic for normal WI-38 cells at therapeutic concentrations. SLN/CnEO substantially enhanced A549 cell death and cell migration inhibition compared with free CnEO.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/160996
url http://sedici.unlp.edu.ar/handle/10915/160996
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1999-4923
info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics15082045
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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