Heterogeneity of circulating prolactin in the bitch
- Autores
- Gobello, María Cristina; Colombani, Miriam; Scaglia, Hugo; Sota, Rodolfo Luzbel de la; Goya, Rodolfo Gustavo
- Año de publicación
- 2001
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL-1, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches.
La prolactine (PRL) circulante se trouve, dans différentes espèces, sous plusieurs formes moléculaires. Compte tenu du fait qu’il n’existe pas d’information disponible chez le chien, nous avons décidé d’étudier le profil moléculaire de la PRL circulante chez cet animal. Nous avons utilisé des pools de sérums provenant de chiennes normales en diestrus (CTRL) ou pseudogestantes (PSPT), ayant destaux élevés ou bas de PRL (> 10 ou < 10 ng·mL-1 respectivement; dosages effectués par Elisa). Les sérums ont été analysés sur colonne de Séphadex G-100 et par chromatographie d’affinité, sur colonne de Sépharose couplée à de la Concanavaline A. La chromatographie sur G-100 a identifié quatre fractions de PRL, qui ont été appelées big-big, big (PM < 67 kDa), native (23 kDa) et fragmentée (< 20 kDa). Les pourcentages relatifs de ces fractions sont identiques chez les CTRL et les PSPT, quel que soit le taux, élevé ou bas, de PRL sérique (plus haut chez les animaux CTRL). De plus, une proportion importante de PRL sérique (69 à 100 %) est sous fonne glycosylée. En conclusion, la PRL sérique se trouve, chez le chien, sous des tonnes moléculaires multiples, dont l’abondance relative est comparable chez la chienne normale et pseudogestante.
Facultad de Ciencias Veterinarias
Instituto de Investigaciones Bioquímicas de La Plata
Facultad de Ciencias Exactas - Materia
-
Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/83506
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Heterogeneity of circulating prolactin in the bitchHétérogénéité de la prolactine circulante chez la chienneGobello, María CristinaColombani, MiriamScaglia, HugoSota, Rodolfo Luzbel de laGoya, Rodolfo GustavoCiencias VeterinariasBitchDiestrusMolecular variantsProlactinPseudopregnancyDifferent molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL<SUP>-1</SUP>, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches.La prolactine (PRL) circulante se trouve, dans différentes espèces, sous plusieurs formes moléculaires. Compte tenu du fait qu’il n’existe pas d’information disponible chez le chien, nous avons décidé d’étudier le profil moléculaire de la PRL circulante chez cet animal. Nous avons utilisé des pools de sérums provenant de chiennes normales en diestrus (CTRL) ou pseudogestantes (PSPT), ayant destaux élevés ou bas de PRL (> 10 ou < 10 ng·mL<SUP>-1</SUP> respectivement; dosages effectués par Elisa). Les sérums ont été analysés sur colonne de Séphadex G-100 et par chromatographie d’affinité, sur colonne de Sépharose couplée à de la Concanavaline A. La chromatographie sur G-100 a identifié quatre fractions de PRL, qui ont été appelées big-big, big (PM < 67 kDa), native (23 kDa) et fragmentée (< 20 kDa). Les pourcentages relatifs de ces fractions sont identiques chez les CTRL et les PSPT, quel que soit le taux, élevé ou bas, de PRL sérique (plus haut chez les animaux CTRL). De plus, une proportion importante de PRL sérique (69 à 100 %) est sous fonne glycosylée. En conclusion, la PRL sérique se trouve, chez le chien, sous des tonnes moléculaires multiples, dont l’abondance relative est comparable chez la chienne normale et pseudogestante.Facultad de Ciencias VeterinariasInstituto de Investigaciones Bioquímicas de La PlataFacultad de Ciencias Exactas2001info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf505-511http://sedici.unlp.edu.ar/handle/10915/83506enginfo:eu-repo/semantics/altIdentifier/issn/0926-5287info:eu-repo/semantics/altIdentifier/doi/10.1051/rnd:2001105info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:50Zoai:sedici.unlp.edu.ar:10915/83506Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:51.002SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Heterogeneity of circulating prolactin in the bitch Hétérogénéité de la prolactine circulante chez la chienne |
title |
Heterogeneity of circulating prolactin in the bitch |
spellingShingle |
Heterogeneity of circulating prolactin in the bitch Gobello, María Cristina Ciencias Veterinarias Bitch Diestrus Molecular variants Prolactin Pseudopregnancy |
title_short |
Heterogeneity of circulating prolactin in the bitch |
title_full |
Heterogeneity of circulating prolactin in the bitch |
title_fullStr |
Heterogeneity of circulating prolactin in the bitch |
title_full_unstemmed |
Heterogeneity of circulating prolactin in the bitch |
title_sort |
Heterogeneity of circulating prolactin in the bitch |
dc.creator.none.fl_str_mv |
Gobello, María Cristina Colombani, Miriam Scaglia, Hugo Sota, Rodolfo Luzbel de la Goya, Rodolfo Gustavo |
author |
Gobello, María Cristina |
author_facet |
Gobello, María Cristina Colombani, Miriam Scaglia, Hugo Sota, Rodolfo Luzbel de la Goya, Rodolfo Gustavo |
author_role |
author |
author2 |
Colombani, Miriam Scaglia, Hugo Sota, Rodolfo Luzbel de la Goya, Rodolfo Gustavo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Bitch Diestrus Molecular variants Prolactin Pseudopregnancy |
topic |
Ciencias Veterinarias Bitch Diestrus Molecular variants Prolactin Pseudopregnancy |
dc.description.none.fl_txt_mv |
Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL<SUP>-1</SUP>, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches. La prolactine (PRL) circulante se trouve, dans différentes espèces, sous plusieurs formes moléculaires. Compte tenu du fait qu’il n’existe pas d’information disponible chez le chien, nous avons décidé d’étudier le profil moléculaire de la PRL circulante chez cet animal. Nous avons utilisé des pools de sérums provenant de chiennes normales en diestrus (CTRL) ou pseudogestantes (PSPT), ayant destaux élevés ou bas de PRL (> 10 ou < 10 ng·mL<SUP>-1</SUP> respectivement; dosages effectués par Elisa). Les sérums ont été analysés sur colonne de Séphadex G-100 et par chromatographie d’affinité, sur colonne de Sépharose couplée à de la Concanavaline A. La chromatographie sur G-100 a identifié quatre fractions de PRL, qui ont été appelées big-big, big (PM < 67 kDa), native (23 kDa) et fragmentée (< 20 kDa). Les pourcentages relatifs de ces fractions sont identiques chez les CTRL et les PSPT, quel que soit le taux, élevé ou bas, de PRL sérique (plus haut chez les animaux CTRL). De plus, une proportion importante de PRL sérique (69 à 100 %) est sous fonne glycosylée. En conclusion, la PRL sérique se trouve, chez le chien, sous des tonnes moléculaires multiples, dont l’abondance relative est comparable chez la chienne normale et pseudogestante. Facultad de Ciencias Veterinarias Instituto de Investigaciones Bioquímicas de La Plata Facultad de Ciencias Exactas |
description |
Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL<SUP>-1</SUP>, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001 |
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eng |
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