Heterogeneity of circulating prolactin in the bitch

Autores
Gobello, María Cristina; Colombani, Miriam; Scaglia, Hugo; Sota, Rodolfo Luzbel de la; Goya, Rodolfo Gustavo
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL-1, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches.
La prolactine (PRL) circulante se trouve, dans différentes espèces, sous plusieurs formes moléculaires. Compte tenu du fait qu’il n’existe pas d’information disponible chez le chien, nous avons décidé d’étudier le profil moléculaire de la PRL circulante chez cet animal. Nous avons utilisé des pools de sérums provenant de chiennes normales en diestrus (CTRL) ou pseudogestantes (PSPT), ayant destaux élevés ou bas de PRL (> 10 ou < 10 ng·mL-1 respectivement; dosages effectués par Elisa). Les sérums ont été analysés sur colonne de Séphadex G-100 et par chromatographie d’affinité, sur colonne de Sépharose couplée à de la Concanavaline A. La chromatographie sur G-100 a identifié quatre fractions de PRL, qui ont été appelées big-big, big (PM < 67 kDa), native (23 kDa) et fragmentée (< 20 kDa). Les pourcentages relatifs de ces fractions sont identiques chez les CTRL et les PSPT, quel que soit le taux, élevé ou bas, de PRL sérique (plus haut chez les animaux CTRL). De plus, une proportion importante de PRL sérique (69 à 100 %) est sous fonne glycosylée. En conclusion, la PRL sérique se trouve, chez le chien, sous des tonnes moléculaires multiples, dont l’abondance relative est comparable chez la chienne normale et pseudogestante.
Facultad de Ciencias Veterinarias
Instituto de Investigaciones Bioquímicas de La Plata
Facultad de Ciencias Exactas
Materia
Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83506

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Heterogeneity of circulating prolactin in the bitchHétérogénéité de la prolactine circulante chez la chienneGobello, María CristinaColombani, MiriamScaglia, HugoSota, Rodolfo Luzbel de laGoya, Rodolfo GustavoCiencias VeterinariasBitchDiestrusMolecular variantsProlactinPseudopregnancyDifferent molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL<SUP>-1</SUP>, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches.La prolactine (PRL) circulante se trouve, dans différentes espèces, sous plusieurs formes moléculaires. Compte tenu du fait qu’il n’existe pas d’information disponible chez le chien, nous avons décidé d’étudier le profil moléculaire de la PRL circulante chez cet animal. Nous avons utilisé des pools de sérums provenant de chiennes normales en diestrus (CTRL) ou pseudogestantes (PSPT), ayant destaux élevés ou bas de PRL (> 10 ou < 10 ng·mL<SUP>-1</SUP> respectivement; dosages effectués par Elisa). Les sérums ont été analysés sur colonne de Séphadex G-100 et par chromatographie d’affinité, sur colonne de Sépharose couplée à de la Concanavaline A. La chromatographie sur G-100 a identifié quatre fractions de PRL, qui ont été appelées big-big, big (PM < 67 kDa), native (23 kDa) et fragmentée (< 20 kDa). Les pourcentages relatifs de ces fractions sont identiques chez les CTRL et les PSPT, quel que soit le taux, élevé ou bas, de PRL sérique (plus haut chez les animaux CTRL). De plus, une proportion importante de PRL sérique (69 à 100 %) est sous fonne glycosylée. En conclusion, la PRL sérique se trouve, chez le chien, sous des tonnes moléculaires multiples, dont l’abondance relative est comparable chez la chienne normale et pseudogestante.Facultad de Ciencias VeterinariasInstituto de Investigaciones Bioquímicas de La PlataFacultad de Ciencias Exactas2001info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf505-511http://sedici.unlp.edu.ar/handle/10915/83506enginfo:eu-repo/semantics/altIdentifier/issn/0926-5287info:eu-repo/semantics/altIdentifier/doi/10.1051/rnd:2001105info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:50Zoai:sedici.unlp.edu.ar:10915/83506Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:51.002SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Heterogeneity of circulating prolactin in the bitch
Hétérogénéité de la prolactine circulante chez la chienne
title Heterogeneity of circulating prolactin in the bitch
spellingShingle Heterogeneity of circulating prolactin in the bitch
Gobello, María Cristina
Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy
title_short Heterogeneity of circulating prolactin in the bitch
title_full Heterogeneity of circulating prolactin in the bitch
title_fullStr Heterogeneity of circulating prolactin in the bitch
title_full_unstemmed Heterogeneity of circulating prolactin in the bitch
title_sort Heterogeneity of circulating prolactin in the bitch
dc.creator.none.fl_str_mv Gobello, María Cristina
Colombani, Miriam
Scaglia, Hugo
Sota, Rodolfo Luzbel de la
Goya, Rodolfo Gustavo
author Gobello, María Cristina
author_facet Gobello, María Cristina
Colombani, Miriam
Scaglia, Hugo
Sota, Rodolfo Luzbel de la
Goya, Rodolfo Gustavo
author_role author
author2 Colombani, Miriam
Scaglia, Hugo
Sota, Rodolfo Luzbel de la
Goya, Rodolfo Gustavo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy
topic Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy
dc.description.none.fl_txt_mv Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL<SUP>-1</SUP>, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches.
La prolactine (PRL) circulante se trouve, dans différentes espèces, sous plusieurs formes moléculaires. Compte tenu du fait qu’il n’existe pas d’information disponible chez le chien, nous avons décidé d’étudier le profil moléculaire de la PRL circulante chez cet animal. Nous avons utilisé des pools de sérums provenant de chiennes normales en diestrus (CTRL) ou pseudogestantes (PSPT), ayant destaux élevés ou bas de PRL (> 10 ou < 10 ng·mL<SUP>-1</SUP> respectivement; dosages effectués par Elisa). Les sérums ont été analysés sur colonne de Séphadex G-100 et par chromatographie d’affinité, sur colonne de Sépharose couplée à de la Concanavaline A. La chromatographie sur G-100 a identifié quatre fractions de PRL, qui ont été appelées big-big, big (PM < 67 kDa), native (23 kDa) et fragmentée (< 20 kDa). Les pourcentages relatifs de ces fractions sont identiques chez les CTRL et les PSPT, quel que soit le taux, élevé ou bas, de PRL sérique (plus haut chez les animaux CTRL). De plus, une proportion importante de PRL sérique (69 à 100 %) est sous fonne glycosylée. En conclusion, la PRL sérique se trouve, chez le chien, sous des tonnes moléculaires multiples, dont l’abondance relative est comparable chez la chienne normale et pseudogestante.
Facultad de Ciencias Veterinarias
Instituto de Investigaciones Bioquímicas de La Plata
Facultad de Ciencias Exactas
description Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL<SUP>-1</SUP>, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches.
publishDate 2001
dc.date.none.fl_str_mv 2001
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:eu-repo/semantics/altIdentifier/doi/10.1051/rnd:2001105
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http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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