DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
- Autores
- Silva, Adriana L.; Martini, Sabrina V.; Abreu, Soraia C.; Samary, Cynthia S.; Diaz, Bruno L.; Fernezlian, Sandra; Sá, Vanessa Karen de; Capelozzi, Vera Luiza; Boylan, Nicholas J.; Goya, Rodolfo Gustavo; Suk, Jung Soo; Rocco, Patricia R. M.; Hanes, Justin; Morales, Marcelo M.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics.
Instituto de Investigaciones Bioquímicas de La Plata - Materia
-
Bioquímica
Allergy gene therapy
Thymulin
DNA nanoparticles
Lung remodeling - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/128275
Ver los metadatos del registro completo
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DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthmaSilva, Adriana L.Martini, Sabrina V.Abreu, Soraia C.Samary, Cynthia S.Diaz, Bruno L.Fernezlian, SandraSá, Vanessa Karen deCapelozzi, Vera LuizaBoylan, Nicholas J.Goya, Rodolfo GustavoSuk, Jung SooRocco, Patricia R. M.Hanes, JustinMorales, Marcelo M.BioquímicaAllergy gene therapyThymulinDNA nanoparticlesLung remodelingThymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics.Instituto de Investigaciones Bioquímicas de La Plata2014-02-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf125-133http://sedici.unlp.edu.ar/handle/10915/128275enginfo:eu-repo/semantics/altIdentifier/issn/1873-4995info:eu-repo/semantics/altIdentifier/issn/0168-3659info:eu-repo/semantics/altIdentifier/pmid/24556417info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jconrel.2014.02.010info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:58Zoai:sedici.unlp.edu.ar:10915/128275Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:59.096SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| spellingShingle |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma Silva, Adriana L. Bioquímica Allergy gene therapy Thymulin DNA nanoparticles Lung remodeling |
| title_short |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_full |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_fullStr |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_full_unstemmed |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_sort |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| dc.creator.none.fl_str_mv |
Silva, Adriana L. Martini, Sabrina V. Abreu, Soraia C. Samary, Cynthia S. Diaz, Bruno L. Fernezlian, Sandra Sá, Vanessa Karen de Capelozzi, Vera Luiza Boylan, Nicholas J. Goya, Rodolfo Gustavo Suk, Jung Soo Rocco, Patricia R. M. Hanes, Justin Morales, Marcelo M. |
| author |
Silva, Adriana L. |
| author_facet |
Silva, Adriana L. Martini, Sabrina V. Abreu, Soraia C. Samary, Cynthia S. Diaz, Bruno L. Fernezlian, Sandra Sá, Vanessa Karen de Capelozzi, Vera Luiza Boylan, Nicholas J. Goya, Rodolfo Gustavo Suk, Jung Soo Rocco, Patricia R. M. Hanes, Justin Morales, Marcelo M. |
| author_role |
author |
| author2 |
Martini, Sabrina V. Abreu, Soraia C. Samary, Cynthia S. Diaz, Bruno L. Fernezlian, Sandra Sá, Vanessa Karen de Capelozzi, Vera Luiza Boylan, Nicholas J. Goya, Rodolfo Gustavo Suk, Jung Soo Rocco, Patricia R. M. Hanes, Justin Morales, Marcelo M. |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Bioquímica Allergy gene therapy Thymulin DNA nanoparticles Lung remodeling |
| topic |
Bioquímica Allergy gene therapy Thymulin DNA nanoparticles Lung remodeling |
| dc.description.none.fl_txt_mv |
Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics. Instituto de Investigaciones Bioquímicas de La Plata |
| description |
Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-02-17 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/128275 |
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eng |
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eng |
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