DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma

Autores
Da Silva, Adriana L.; Martini, Sabrina V.; Abreu, Soraia C.; Samary, Cynthia dos S.; Diaz, Bruno L.; Fernezlian, Sandra; de Sa, Vanessa Karen; Capelozzi, Vera Luiza; Boylan, Nicholas J.; Goya, Rodolfo Gustavo; Suk, Jung Soo; Rocco, Patricia R.M.; Hanes, Justin; Morales, Marcelo M.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27 days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics.
Fil: Da Silva, Adriana L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Martini, Sabrina V.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Abreu, Soraia C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Samary, Cynthia dos S.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Diaz, Bruno L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Fernezlian, Sandra. Universidade de Sao Paulo; Brasil
Fil: de Sa, Vanessa Karen. Universidade de Sao Paulo; Brasil
Fil: Capelozzi, Vera Luiza. Universidade de Sao Paulo; Brasil
Fil: Boylan, Nicholas J.. University Johns Hopkins; Estados Unidos
Fil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Suk, Jung Soo. University Johns Hopkins; Estados Unidos
Fil: Rocco, Patricia R.M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Hanes, Justin. University Johns Hopkins; Estados Unidos
Fil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; Brasil
Materia
Allergy Gene Therapy
Thymulin
Dna Nanoparticles
Lung Remodeling
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24120
Acceder
id CONICETDig_1399d044d43094f8485449d699dc3b67
oai_identifier_str oai:ri.conicet.gov.ar:11336/24120
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthmaDa Silva, Adriana L.Martini, Sabrina V.Abreu, Soraia C.Samary, Cynthia dos S.Diaz, Bruno L.Fernezlian, Sandrade Sa, Vanessa KarenCapelozzi, Vera LuizaBoylan, Nicholas J.Goya, Rodolfo GustavoSuk, Jung SooRocco, Patricia R.M.Hanes, JustinMorales, Marcelo M.Allergy Gene TherapyThymulinDna NanoparticlesLung Remodelinghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27 days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics.Fil: Da Silva, Adriana L.. Universidade Federal do Rio de Janeiro; BrasilFil: Martini, Sabrina V.. Universidade Federal do Rio de Janeiro; BrasilFil: Abreu, Soraia C.. Universidade Federal do Rio de Janeiro; BrasilFil: Samary, Cynthia dos S.. Universidade Federal do Rio de Janeiro; BrasilFil: Diaz, Bruno L.. Universidade Federal do Rio de Janeiro; BrasilFil: Fernezlian, Sandra. Universidade de Sao Paulo; BrasilFil: de Sa, Vanessa Karen. Universidade de Sao Paulo; BrasilFil: Capelozzi, Vera Luiza. Universidade de Sao Paulo; BrasilFil: Boylan, Nicholas J.. University Johns Hopkins; Estados UnidosFil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Suk, Jung Soo. University Johns Hopkins; Estados UnidosFil: Rocco, Patricia R.M.. Universidade Federal do Rio de Janeiro; BrasilFil: Hanes, Justin. University Johns Hopkins; Estados UnidosFil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; BrasilElsevier Science2013-04-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24120Da Silva, Adriana L.; Martini, Sabrina V.; Abreu, Soraia C.; Samary, Cynthia dos S.; Diaz, Bruno L.; et al.; DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma; Elsevier Science; Journal Of Controlled Release; 180; 28-4-2013; 125-1330168-3659CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jconrel.2014.02.010info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168365914000984info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:24Zoai:ri.conicet.gov.ar:11336/24120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:24.831CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
title DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
spellingShingle DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
Da Silva, Adriana L.
Allergy Gene Therapy
Thymulin
Dna Nanoparticles
Lung Remodeling
title_short DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
title_full DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
title_fullStr DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
title_full_unstemmed DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
title_sort DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
dc.creator.none.fl_str_mv Da Silva, Adriana L.
Martini, Sabrina V.
Abreu, Soraia C.
Samary, Cynthia dos S.
Diaz, Bruno L.
Fernezlian, Sandra
de Sa, Vanessa Karen
Capelozzi, Vera Luiza
Boylan, Nicholas J.
Goya, Rodolfo Gustavo
Suk, Jung Soo
Rocco, Patricia R.M.
Hanes, Justin
Morales, Marcelo M.
author Da Silva, Adriana L.
author_facet Da Silva, Adriana L.
Martini, Sabrina V.
Abreu, Soraia C.
Samary, Cynthia dos S.
Diaz, Bruno L.
Fernezlian, Sandra
de Sa, Vanessa Karen
Capelozzi, Vera Luiza
Boylan, Nicholas J.
Goya, Rodolfo Gustavo
Suk, Jung Soo
Rocco, Patricia R.M.
Hanes, Justin
Morales, Marcelo M.
author_role author
author2 Martini, Sabrina V.
Abreu, Soraia C.
Samary, Cynthia dos S.
Diaz, Bruno L.
Fernezlian, Sandra
de Sa, Vanessa Karen
Capelozzi, Vera Luiza
Boylan, Nicholas J.
Goya, Rodolfo Gustavo
Suk, Jung Soo
Rocco, Patricia R.M.
Hanes, Justin
Morales, Marcelo M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Allergy Gene Therapy
Thymulin
Dna Nanoparticles
Lung Remodeling
topic Allergy Gene Therapy
Thymulin
Dna Nanoparticles
Lung Remodeling
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27 days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics.
Fil: Da Silva, Adriana L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Martini, Sabrina V.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Abreu, Soraia C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Samary, Cynthia dos S.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Diaz, Bruno L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Fernezlian, Sandra. Universidade de Sao Paulo; Brasil
Fil: de Sa, Vanessa Karen. Universidade de Sao Paulo; Brasil
Fil: Capelozzi, Vera Luiza. Universidade de Sao Paulo; Brasil
Fil: Boylan, Nicholas J.. University Johns Hopkins; Estados Unidos
Fil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Suk, Jung Soo. University Johns Hopkins; Estados Unidos
Fil: Rocco, Patricia R.M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Hanes, Justin. University Johns Hopkins; Estados Unidos
Fil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; Brasil
description Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27 days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24120
Da Silva, Adriana L.; Martini, Sabrina V.; Abreu, Soraia C.; Samary, Cynthia dos S.; Diaz, Bruno L.; et al.; DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma; Elsevier Science; Journal Of Controlled Release; 180; 28-4-2013; 125-133
0168-3659
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24120
identifier_str_mv Da Silva, Adriana L.; Martini, Sabrina V.; Abreu, Soraia C.; Samary, Cynthia dos S.; Diaz, Bruno L.; et al.; DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma; Elsevier Science; Journal Of Controlled Release; 180; 28-4-2013; 125-133
0168-3659
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jconrel.2014.02.010
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168365914000984
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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