Emulsan quantitation by Nile red quenching fluorescence assay

Autores
Castro, Guillermo Raúl; Larson, Bridget K.; Panilaitis, Bruce; Kaplan, David L.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG⁻¹.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Biología
Emulsan
Polyhydroxyalkanoates
Emulsan concentration
Turbidimetric assay
Polyhydroxyalkanoic acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/153222
Acceder
id SEDICI_0844e94932a9aab16a6b3d86376a744e
oai_identifier_str oai:sedici.unlp.edu.ar:10915/153222
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Emulsan quantitation by Nile red quenching fluorescence assayCastro, Guillermo RaúlLarson, Bridget K.Panilaitis, BruceKaplan, David L.BiologíaEmulsanPolyhydroxyalkanoatesEmulsan concentrationTurbidimetric assayPolyhydroxyalkanoic acidA Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG⁻¹.Centro de Investigación y Desarrollo en Fermentaciones Industriales2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf767–770http://sedici.unlp.edu.ar/handle/10915/153222enginfo:eu-repo/semantics/altIdentifier/issn/0175-7598info:eu-repo/semantics/altIdentifier/issn/1432-0614info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-004-1849-2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:11:38Zoai:sedici.unlp.edu.ar:10915/153222Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:11:39.12SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Emulsan quantitation by Nile red quenching fluorescence assay
title Emulsan quantitation by Nile red quenching fluorescence assay
spellingShingle Emulsan quantitation by Nile red quenching fluorescence assay
Castro, Guillermo Raúl
Biología
Emulsan
Polyhydroxyalkanoates
Emulsan concentration
Turbidimetric assay
Polyhydroxyalkanoic acid
title_short Emulsan quantitation by Nile red quenching fluorescence assay
title_full Emulsan quantitation by Nile red quenching fluorescence assay
title_fullStr Emulsan quantitation by Nile red quenching fluorescence assay
title_full_unstemmed Emulsan quantitation by Nile red quenching fluorescence assay
title_sort Emulsan quantitation by Nile red quenching fluorescence assay
dc.creator.none.fl_str_mv Castro, Guillermo Raúl
Larson, Bridget K.
Panilaitis, Bruce
Kaplan, David L.
author Castro, Guillermo Raúl
author_facet Castro, Guillermo Raúl
Larson, Bridget K.
Panilaitis, Bruce
Kaplan, David L.
author_role author
author2 Larson, Bridget K.
Panilaitis, Bruce
Kaplan, David L.
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Emulsan
Polyhydroxyalkanoates
Emulsan concentration
Turbidimetric assay
Polyhydroxyalkanoic acid
topic Biología
Emulsan
Polyhydroxyalkanoates
Emulsan concentration
Turbidimetric assay
Polyhydroxyalkanoic acid
dc.description.none.fl_txt_mv A Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG⁻¹.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description A Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG⁻¹.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/153222
url http://sedici.unlp.edu.ar/handle/10915/153222
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0175-7598
info:eu-repo/semantics/altIdentifier/issn/1432-0614
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-004-1849-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
767–770
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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