Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface

Autores
Merino, Lina Ethel; Noto Llana, Mariángeles; Garófalo, Ailín; Trejo, Fernando Miguel; Golowczyc, Marina Alejandra
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Salmonella enteritidis (SE) is a pathogenic bacterium commonly found on the surface of eggshells. In this study, we investigated the biofilm formation of a specific strain of S. enteritidis, CIDCA 115 (SE 115) on eggshells. Additionally, we examined the impact of Lentilactobacillus kefiri strains 83113 and 8321, as well as Lactiplantibacillus plantarum 83114, and their metabolites present in the supernatant on the biofilm formation of SE 115. Scanning electron microscopy revealed that SE 115 formed a mature biofilm structure on the eggshell. During coincubation and pre-incubation, lactic acid bacteria strains significantly reduced the formation of SE 115 biofilm (p < 0.05 and p < 0.01, respectively) compared to SE 115 grown alone. The cell-free supernatants of lactic acid bacteria also exhibited a reduction in SE 115 biofilm formation and modified its structure. Co-incubation with SE 115 induced alterations in the composition of biofilm matrix components, notably in the levels of fimbria curli and cellulose. The qPCR analysis revealed that, after 48 h of incubation, the expression of the csgD gene, a critical regulator of biofilm formation, remained unchanged compared to planktonic cells. However, genes associated with the production of biofilm matrix components, curli (csgA) and cellulose (bcsA), exhibited heightened expression in the presence of lactic acid bacteria compared to the planktonic state. This study highlights the potential of lactic acid bacteria strains and their metabolites as innovative strategies for managing Salmonella biofilm formation in the context of poultry production.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Bioquímica
Salmonella
Biofilm
Eggshell
Lactic acid bacteria
Gene regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/181848
Acceder
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network_name_str SEDICI (UNLP)
spelling Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surfaceMerino, Lina EthelNoto Llana, MariángelesGarófalo, AilínTrejo, Fernando MiguelGolowczyc, Marina AlejandraBioquímicaSalmonellaBiofilmEggshellLactic acid bacteriaGene regulationSalmonella enteritidis (SE) is a pathogenic bacterium commonly found on the surface of eggshells. In this study, we investigated the biofilm formation of a specific strain of S. enteritidis, CIDCA 115 (SE 115) on eggshells. Additionally, we examined the impact of Lentilactobacillus kefiri strains 83113 and 8321, as well as Lactiplantibacillus plantarum 83114, and their metabolites present in the supernatant on the biofilm formation of SE 115. Scanning electron microscopy revealed that SE 115 formed a mature biofilm structure on the eggshell. During coincubation and pre-incubation, lactic acid bacteria strains significantly reduced the formation of SE 115 biofilm (p < 0.05 and p < 0.01, respectively) compared to SE 115 grown alone. The cell-free supernatants of lactic acid bacteria also exhibited a reduction in SE 115 biofilm formation and modified its structure. Co-incubation with SE 115 induced alterations in the composition of biofilm matrix components, notably in the levels of fimbria curli and cellulose. The qPCR analysis revealed that, after 48 h of incubation, the expression of the csgD gene, a critical regulator of biofilm formation, remained unchanged compared to planktonic cells. However, genes associated with the production of biofilm matrix components, curli (csgA) and cellulose (bcsA), exhibited heightened expression in the presence of lactic acid bacteria compared to the planktonic state. This study highlights the potential of lactic acid bacteria strains and their metabolites as innovative strategies for managing Salmonella biofilm formation in the context of poultry production.Centro de Investigación y Desarrollo en Criotecnología de Alimentos2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/181848enginfo:eu-repo/semantics/altIdentifier/issn/2666-5174info:eu-repo/semantics/altIdentifier/doi/10.1016/j.crmicr.2025.100384info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:49:38Zoai:sedici.unlp.edu.ar:10915/181848Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:49:38.987SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface
title Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface
spellingShingle Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface
Merino, Lina Ethel
Bioquímica
Salmonella
Biofilm
Eggshell
Lactic acid bacteria
Gene regulation
title_short Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface
title_full Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface
title_fullStr Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface
title_full_unstemmed Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface
title_sort Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface
dc.creator.none.fl_str_mv Merino, Lina Ethel
Noto Llana, Mariángeles
Garófalo, Ailín
Trejo, Fernando Miguel
Golowczyc, Marina Alejandra
author Merino, Lina Ethel
author_facet Merino, Lina Ethel
Noto Llana, Mariángeles
Garófalo, Ailín
Trejo, Fernando Miguel
Golowczyc, Marina Alejandra
author_role author
author2 Noto Llana, Mariángeles
Garófalo, Ailín
Trejo, Fernando Miguel
Golowczyc, Marina Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Salmonella
Biofilm
Eggshell
Lactic acid bacteria
Gene regulation
topic Bioquímica
Salmonella
Biofilm
Eggshell
Lactic acid bacteria
Gene regulation
dc.description.none.fl_txt_mv Salmonella enteritidis (SE) is a pathogenic bacterium commonly found on the surface of eggshells. In this study, we investigated the biofilm formation of a specific strain of S. enteritidis, CIDCA 115 (SE 115) on eggshells. Additionally, we examined the impact of Lentilactobacillus kefiri strains 83113 and 8321, as well as Lactiplantibacillus plantarum 83114, and their metabolites present in the supernatant on the biofilm formation of SE 115. Scanning electron microscopy revealed that SE 115 formed a mature biofilm structure on the eggshell. During coincubation and pre-incubation, lactic acid bacteria strains significantly reduced the formation of SE 115 biofilm (p < 0.05 and p < 0.01, respectively) compared to SE 115 grown alone. The cell-free supernatants of lactic acid bacteria also exhibited a reduction in SE 115 biofilm formation and modified its structure. Co-incubation with SE 115 induced alterations in the composition of biofilm matrix components, notably in the levels of fimbria curli and cellulose. The qPCR analysis revealed that, after 48 h of incubation, the expression of the csgD gene, a critical regulator of biofilm formation, remained unchanged compared to planktonic cells. However, genes associated with the production of biofilm matrix components, curli (csgA) and cellulose (bcsA), exhibited heightened expression in the presence of lactic acid bacteria compared to the planktonic state. This study highlights the potential of lactic acid bacteria strains and their metabolites as innovative strategies for managing Salmonella biofilm formation in the context of poultry production.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description Salmonella enteritidis (SE) is a pathogenic bacterium commonly found on the surface of eggshells. In this study, we investigated the biofilm formation of a specific strain of S. enteritidis, CIDCA 115 (SE 115) on eggshells. Additionally, we examined the impact of Lentilactobacillus kefiri strains 83113 and 8321, as well as Lactiplantibacillus plantarum 83114, and their metabolites present in the supernatant on the biofilm formation of SE 115. Scanning electron microscopy revealed that SE 115 formed a mature biofilm structure on the eggshell. During coincubation and pre-incubation, lactic acid bacteria strains significantly reduced the formation of SE 115 biofilm (p < 0.05 and p < 0.01, respectively) compared to SE 115 grown alone. The cell-free supernatants of lactic acid bacteria also exhibited a reduction in SE 115 biofilm formation and modified its structure. Co-incubation with SE 115 induced alterations in the composition of biofilm matrix components, notably in the levels of fimbria curli and cellulose. The qPCR analysis revealed that, after 48 h of incubation, the expression of the csgD gene, a critical regulator of biofilm formation, remained unchanged compared to planktonic cells. However, genes associated with the production of biofilm matrix components, curli (csgA) and cellulose (bcsA), exhibited heightened expression in the presence of lactic acid bacteria compared to the planktonic state. This study highlights the potential of lactic acid bacteria strains and their metabolites as innovative strategies for managing Salmonella biofilm formation in the context of poultry production.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/181848
url http://sedici.unlp.edu.ar/handle/10915/181848
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2666-5174
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.crmicr.2025.100384
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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