Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae

Autores
Tranchida, María Cecilia; Riccillo, Pablo Miguel; Micieli, María Victoria; García, Juan José; Rodriguero, Marcela Silvina
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Three strains—C107, C207, and C307—of spore-forming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC₅₀, 4 × 10⁴ spores/ml), while O. albifasciatus was most susceptible to C207 (LC₅₀, 3.4 × 10⁶ spores/ml).
Facultad de Ciencias Naturales y Museo
Centro de Estudios Parasitológicos y de Vectores
Materia
Ciencias Naturales
Mosquito larvae
Culex pipiens
Lysinibacillus sphaericus
Biological control
16S rRNA gene
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/134704
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/134704
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvaeTranchida, María CeciliaRiccillo, Pablo MiguelMicieli, María VictoriaGarcía, Juan JoséRodriguero, Marcela SilvinaCiencias NaturalesMosquito larvaeCulex pipiensLysinibacillus sphaericusBiological control16S rRNA geneThree strains—C107, C207, and C307—of spore-forming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of <i>Culex pipiens</i> in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of <i>Lysinibacillus sphaericus</i>, while C307 was related to <i>Bacillus licheniformis</i>. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the <i>binA</i>, <i>binB</i>, and <i>mtx</i> genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: <i>Culex pipiens</i>, <i>Aedes aegypti</i>, <i>Culex dolosus</i>, <i>Culex apicinus</i>, <i>Ochlerotatus albifasciatus</i>, and <i>Anopheles albitarsis</i>. Only isolates C107 and C207 exhibited mosquitocidal activity. <i>Culex pipiens</i> was the species most susceptible to C107 (LC₅₀, 4 × 10⁴ spores/ml), while <i>O. albifasciatus</i> was most susceptible to C207 (LC₅₀, 3.4 × 10⁶ spores/ml).Facultad de Ciencias Naturales y MuseoCentro de Estudios Parasitológicos y de Vectores2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf575-584http://sedici.unlp.edu.ar/handle/10915/134704enginfo:eu-repo/semantics/altIdentifier/issn/1590-4261info:eu-repo/semantics/altIdentifier/issn/1869-2044info:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-010-0175-4info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:03:59Zoai:sedici.unlp.edu.ar:10915/134704Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:03:59.734SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
spellingShingle Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
Tranchida, María Cecilia
Ciencias Naturales
Mosquito larvae
Culex pipiens
Lysinibacillus sphaericus
Biological control
16S rRNA gene
title_short Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_full Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_fullStr Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_full_unstemmed Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_sort Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
dc.creator.none.fl_str_mv Tranchida, María Cecilia
Riccillo, Pablo Miguel
Micieli, María Victoria
García, Juan José
Rodriguero, Marcela Silvina
author Tranchida, María Cecilia
author_facet Tranchida, María Cecilia
Riccillo, Pablo Miguel
Micieli, María Victoria
García, Juan José
Rodriguero, Marcela Silvina
author_role author
author2 Riccillo, Pablo Miguel
Micieli, María Victoria
García, Juan José
Rodriguero, Marcela Silvina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Mosquito larvae
Culex pipiens
Lysinibacillus sphaericus
Biological control
16S rRNA gene
topic Ciencias Naturales
Mosquito larvae
Culex pipiens
Lysinibacillus sphaericus
Biological control
16S rRNA gene
dc.description.none.fl_txt_mv Three strains—C107, C207, and C307—of spore-forming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of <i>Culex pipiens</i> in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of <i>Lysinibacillus sphaericus</i>, while C307 was related to <i>Bacillus licheniformis</i>. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the <i>binA</i>, <i>binB</i>, and <i>mtx</i> genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: <i>Culex pipiens</i>, <i>Aedes aegypti</i>, <i>Culex dolosus</i>, <i>Culex apicinus</i>, <i>Ochlerotatus albifasciatus</i>, and <i>Anopheles albitarsis</i>. Only isolates C107 and C207 exhibited mosquitocidal activity. <i>Culex pipiens</i> was the species most susceptible to C107 (LC₅₀, 4 × 10⁴ spores/ml), while <i>O. albifasciatus</i> was most susceptible to C207 (LC₅₀, 3.4 × 10⁶ spores/ml).
Facultad de Ciencias Naturales y Museo
Centro de Estudios Parasitológicos y de Vectores
description Three strains—C107, C207, and C307—of spore-forming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of <i>Culex pipiens</i> in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of <i>Lysinibacillus sphaericus</i>, while C307 was related to <i>Bacillus licheniformis</i>. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the <i>binA</i>, <i>binB</i>, and <i>mtx</i> genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: <i>Culex pipiens</i>, <i>Aedes aegypti</i>, <i>Culex dolosus</i>, <i>Culex apicinus</i>, <i>Ochlerotatus albifasciatus</i>, and <i>Anopheles albitarsis</i>. Only isolates C107 and C207 exhibited mosquitocidal activity. <i>Culex pipiens</i> was the species most susceptible to C107 (LC₅₀, 4 × 10⁴ spores/ml), while <i>O. albifasciatus</i> was most susceptible to C207 (LC₅₀, 3.4 × 10⁶ spores/ml).
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/134704
url http://sedici.unlp.edu.ar/handle/10915/134704
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1590-4261
info:eu-repo/semantics/altIdentifier/issn/1869-2044
info:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-010-0175-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
575-584
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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