Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae

Autores
Tranchida, Maria Cecilia; Riccillo, Pablo M.; Micieli, Maria Victoria; García, Juan J.; Rodriguero, Marcela Silvina
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC50, 4× 104 spores/ml), while O. albifasciatus was most susceptible to C207 (LC 50, 3.4× 106 spores/ml). © Springer-Verlag and the University of Milan 2010.
Fil: Tranchida, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Riccillo, Pablo M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: García, Juan J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Rodriguero, Marcela Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Materia
16s Rrna Gene
Biological Control
Culex Pipiens
Lysinibacillus Sphaericus
Mosquito Larvae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72601
Acceder
id CONICETDig_c74598cc903f214594a84f1e29482a3a
oai_identifier_str oai:ri.conicet.gov.ar:11336/72601
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvaeTranchida, Maria CeciliaRiccillo, Pablo M.Micieli, Maria VictoriaGarcía, Juan J.Rodriguero, Marcela Silvina16s Rrna GeneBiological ControlCulex PipiensLysinibacillus SphaericusMosquito Larvaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC50, 4× 104 spores/ml), while O. albifasciatus was most susceptible to C207 (LC 50, 3.4× 106 spores/ml). © Springer-Verlag and the University of Milan 2010.Fil: Tranchida, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Riccillo, Pablo M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: García, Juan J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Rodriguero, Marcela Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaInst Microbiologia2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72601Tranchida, Maria Cecilia; Riccillo, Pablo M.; Micieli, Maria Victoria; García, Juan J.; Rodriguero, Marcela Silvina; Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae; Inst Microbiologia; Annals Of Microbiology; 61; 3; 4-2011; 575-5841590-4261CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-010-0175-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13213-010-0175-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:17Zoai:ri.conicet.gov.ar:11336/72601instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:17.814CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
spellingShingle Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
Tranchida, Maria Cecilia
16s Rrna Gene
Biological Control
Culex Pipiens
Lysinibacillus Sphaericus
Mosquito Larvae
title_short Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_full Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_fullStr Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_full_unstemmed Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_sort Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
dc.creator.none.fl_str_mv Tranchida, Maria Cecilia
Riccillo, Pablo M.
Micieli, Maria Victoria
García, Juan J.
Rodriguero, Marcela Silvina
author Tranchida, Maria Cecilia
author_facet Tranchida, Maria Cecilia
Riccillo, Pablo M.
Micieli, Maria Victoria
García, Juan J.
Rodriguero, Marcela Silvina
author_role author
author2 Riccillo, Pablo M.
Micieli, Maria Victoria
García, Juan J.
Rodriguero, Marcela Silvina
author2_role author
author
author
author
dc.subject.none.fl_str_mv 16s Rrna Gene
Biological Control
Culex Pipiens
Lysinibacillus Sphaericus
Mosquito Larvae
topic 16s Rrna Gene
Biological Control
Culex Pipiens
Lysinibacillus Sphaericus
Mosquito Larvae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC50, 4× 104 spores/ml), while O. albifasciatus was most susceptible to C207 (LC 50, 3.4× 106 spores/ml). © Springer-Verlag and the University of Milan 2010.
Fil: Tranchida, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Riccillo, Pablo M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: García, Juan J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Rodriguero, Marcela Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
description Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC50, 4× 104 spores/ml), while O. albifasciatus was most susceptible to C207 (LC 50, 3.4× 106 spores/ml). © Springer-Verlag and the University of Milan 2010.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72601
Tranchida, Maria Cecilia; Riccillo, Pablo M.; Micieli, Maria Victoria; García, Juan J.; Rodriguero, Marcela Silvina; Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae; Inst Microbiologia; Annals Of Microbiology; 61; 3; 4-2011; 575-584
1590-4261
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72601
identifier_str_mv Tranchida, Maria Cecilia; Riccillo, Pablo M.; Micieli, Maria Victoria; García, Juan J.; Rodriguero, Marcela Silvina; Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae; Inst Microbiologia; Annals Of Microbiology; 61; 3; 4-2011; 575-584
1590-4261
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-010-0175-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13213-010-0175-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inst Microbiologia
publisher.none.fl_str_mv Inst Microbiologia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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