Release of outer membrane vesicles from Bordetella pertussis

Autores
Hozbor, Daniela Flavia; Rodríguez, María Eugenia; Fernández, Julieta; Lagares, Antonio; Guiso, Nicole; Yantorno, Osvaldo Miguel
Año de publicación
1999
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly− showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnología y Biología Molecular
Materia
Biología
Polypeptide
Liquid Medium
Outer Membrane
Basic Material
Pertussis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/137514
Acceder
id SEDICI_03fef4460ae8ae9bace886154579abb8
oai_identifier_str oai:sedici.unlp.edu.ar:10915/137514
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Release of outer membrane vesicles from Bordetella pertussisHozbor, Daniela FlaviaRodríguez, María EugeniaFernández, JulietaLagares, AntonioGuiso, NicoleYantorno, Osvaldo MiguelBiologíaPolypeptideLiquid MediumOuter MembraneBasic MaterialPertussisThe aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly− showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesInstituto de Biotecnología y Biología Molecular1999info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf273-278http://sedici.unlp.edu.ar/handle/10915/137514enginfo:eu-repo/semantics/altIdentifier/issn/0343-8651info:eu-repo/semantics/altIdentifier/issn/1432-0991info:eu-repo/semantics/altIdentifier/doi/10.1007/pl00006801info:eu-repo/semantics/altIdentifier/pmid/10355115info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:16Zoai:sedici.unlp.edu.ar:10915/137514Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:17.268SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Release of outer membrane vesicles from Bordetella pertussis
title Release of outer membrane vesicles from Bordetella pertussis
spellingShingle Release of outer membrane vesicles from Bordetella pertussis
Hozbor, Daniela Flavia
Biología
Polypeptide
Liquid Medium
Outer Membrane
Basic Material
Pertussis
title_short Release of outer membrane vesicles from Bordetella pertussis
title_full Release of outer membrane vesicles from Bordetella pertussis
title_fullStr Release of outer membrane vesicles from Bordetella pertussis
title_full_unstemmed Release of outer membrane vesicles from Bordetella pertussis
title_sort Release of outer membrane vesicles from Bordetella pertussis
dc.creator.none.fl_str_mv Hozbor, Daniela Flavia
Rodríguez, María Eugenia
Fernández, Julieta
Lagares, Antonio
Guiso, Nicole
Yantorno, Osvaldo Miguel
author Hozbor, Daniela Flavia
author_facet Hozbor, Daniela Flavia
Rodríguez, María Eugenia
Fernández, Julieta
Lagares, Antonio
Guiso, Nicole
Yantorno, Osvaldo Miguel
author_role author
author2 Rodríguez, María Eugenia
Fernández, Julieta
Lagares, Antonio
Guiso, Nicole
Yantorno, Osvaldo Miguel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Polypeptide
Liquid Medium
Outer Membrane
Basic Material
Pertussis
topic Biología
Polypeptide
Liquid Medium
Outer Membrane
Basic Material
Pertussis
dc.description.none.fl_txt_mv The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly− showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnología y Biología Molecular
description The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly− showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines.
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/137514
url http://sedici.unlp.edu.ar/handle/10915/137514
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0343-8651
info:eu-repo/semantics/altIdentifier/issn/1432-0991
info:eu-repo/semantics/altIdentifier/doi/10.1007/pl00006801
info:eu-repo/semantics/altIdentifier/pmid/10355115
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
273-278
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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