Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis

Autores
Elizagaray, Maia Lina; Gomes, Marco Túlio R.; Guimaraes, Erika S.; Rumbo, Martín; Hozbor, Daniela Flavia; Oliveira, Sergio C.; Moreno, Griselda Noemí
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Outer Membrane Vesicles (OMVs) derived from different Gram-negative bacteria have been proposed as an attractive vaccine platform because of their own immunogenic adjuvant properties. Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the last decades in many countries. In response to the epidemiological situation, new boosters have been incorporated into vaccination schedules worldwide and new vaccine candidates have started to be designed. Particularly, our group designed a new pertussis vaccine candidate based on OMVs derived from Bordetella pertussis (BpOMVs). To continue with the characterization of the immune response induced by our OMV based vaccine candidate, this work aimed to investigate the ability of OMVs to activate the inflammasome pathway in macrophages. We observed that NLRP3, caspase-1/11, and gasdermin-D (GSDMD) are involved in inflammasome activation by BpOMVs. Moreover, we demonstrated that BpOMVs as well as transfected B. pertussis lipooligosaccharide (BpLOS) induce caspase-11 (Casp11) and guanylate-binding proteins (GBPs) dependent non-canonical inflammasome activation. Our results elucidate the mechanism by which BpOMVs trigger one central pathway of the innate response activation that is expected to skew the adaptive immune response elicited by BpOMVs vaccination.
Fil: Elizagaray, Maia Lina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Gomes, Marco Túlio R.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Guimaraes, Erika S.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Oliveira, Sergio C.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Moreno, Griselda Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Materia
INFLAMMASOME
CASPASES1/11
BORDETELLA PERTUSSIS
OUTER MEMBRANE VESICLES
LOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154545
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154545
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussisElizagaray, Maia LinaGomes, Marco Túlio R.Guimaraes, Erika S.Rumbo, MartínHozbor, Daniela FlaviaOliveira, Sergio C.Moreno, Griselda NoemíINFLAMMASOMECASPASES1/11BORDETELLA PERTUSSISOUTER MEMBRANE VESICLESLOShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Outer Membrane Vesicles (OMVs) derived from different Gram-negative bacteria have been proposed as an attractive vaccine platform because of their own immunogenic adjuvant properties. Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the last decades in many countries. In response to the epidemiological situation, new boosters have been incorporated into vaccination schedules worldwide and new vaccine candidates have started to be designed. Particularly, our group designed a new pertussis vaccine candidate based on OMVs derived from Bordetella pertussis (BpOMVs). To continue with the characterization of the immune response induced by our OMV based vaccine candidate, this work aimed to investigate the ability of OMVs to activate the inflammasome pathway in macrophages. We observed that NLRP3, caspase-1/11, and gasdermin-D (GSDMD) are involved in inflammasome activation by BpOMVs. Moreover, we demonstrated that BpOMVs as well as transfected B. pertussis lipooligosaccharide (BpLOS) induce caspase-11 (Casp11) and guanylate-binding proteins (GBPs) dependent non-canonical inflammasome activation. Our results elucidate the mechanism by which BpOMVs trigger one central pathway of the innate response activation that is expected to skew the adaptive immune response elicited by BpOMVs vaccination.Fil: Elizagaray, Maia Lina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Gomes, Marco Túlio R.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFil: Guimaraes, Erika S.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Oliveira, Sergio C.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFil: Moreno, Griselda Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFrontiers Media2020-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154545Elizagaray, Maia Lina; Gomes, Marco Túlio R.; Guimaraes, Erika S.; Rumbo, Martín; Hozbor, Daniela Flavia; et al.; Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis; Frontiers Media; Frontiers in Immunology; 11; 20-8-2020; 1-131664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fimmu.2020.01879/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2020.01879info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:03Zoai:ri.conicet.gov.ar:11336/154545instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:03.509CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis
title Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis
spellingShingle Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis
Elizagaray, Maia Lina
INFLAMMASOME
CASPASES1/11
BORDETELLA PERTUSSIS
OUTER MEMBRANE VESICLES
LOS
title_short Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis
title_full Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis
title_fullStr Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis
title_full_unstemmed Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis
title_sort Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis
dc.creator.none.fl_str_mv Elizagaray, Maia Lina
Gomes, Marco Túlio R.
Guimaraes, Erika S.
Rumbo, Martín
Hozbor, Daniela Flavia
Oliveira, Sergio C.
Moreno, Griselda Noemí
author Elizagaray, Maia Lina
author_facet Elizagaray, Maia Lina
Gomes, Marco Túlio R.
Guimaraes, Erika S.
Rumbo, Martín
Hozbor, Daniela Flavia
Oliveira, Sergio C.
Moreno, Griselda Noemí
author_role author
author2 Gomes, Marco Túlio R.
Guimaraes, Erika S.
Rumbo, Martín
Hozbor, Daniela Flavia
Oliveira, Sergio C.
Moreno, Griselda Noemí
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv INFLAMMASOME
CASPASES1/11
BORDETELLA PERTUSSIS
OUTER MEMBRANE VESICLES
LOS
topic INFLAMMASOME
CASPASES1/11
BORDETELLA PERTUSSIS
OUTER MEMBRANE VESICLES
LOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Outer Membrane Vesicles (OMVs) derived from different Gram-negative bacteria have been proposed as an attractive vaccine platform because of their own immunogenic adjuvant properties. Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the last decades in many countries. In response to the epidemiological situation, new boosters have been incorporated into vaccination schedules worldwide and new vaccine candidates have started to be designed. Particularly, our group designed a new pertussis vaccine candidate based on OMVs derived from Bordetella pertussis (BpOMVs). To continue with the characterization of the immune response induced by our OMV based vaccine candidate, this work aimed to investigate the ability of OMVs to activate the inflammasome pathway in macrophages. We observed that NLRP3, caspase-1/11, and gasdermin-D (GSDMD) are involved in inflammasome activation by BpOMVs. Moreover, we demonstrated that BpOMVs as well as transfected B. pertussis lipooligosaccharide (BpLOS) induce caspase-11 (Casp11) and guanylate-binding proteins (GBPs) dependent non-canonical inflammasome activation. Our results elucidate the mechanism by which BpOMVs trigger one central pathway of the innate response activation that is expected to skew the adaptive immune response elicited by BpOMVs vaccination.
Fil: Elizagaray, Maia Lina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Gomes, Marco Túlio R.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Guimaraes, Erika S.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Oliveira, Sergio C.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Moreno, Griselda Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
description Outer Membrane Vesicles (OMVs) derived from different Gram-negative bacteria have been proposed as an attractive vaccine platform because of their own immunogenic adjuvant properties. Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the last decades in many countries. In response to the epidemiological situation, new boosters have been incorporated into vaccination schedules worldwide and new vaccine candidates have started to be designed. Particularly, our group designed a new pertussis vaccine candidate based on OMVs derived from Bordetella pertussis (BpOMVs). To continue with the characterization of the immune response induced by our OMV based vaccine candidate, this work aimed to investigate the ability of OMVs to activate the inflammasome pathway in macrophages. We observed that NLRP3, caspase-1/11, and gasdermin-D (GSDMD) are involved in inflammasome activation by BpOMVs. Moreover, we demonstrated that BpOMVs as well as transfected B. pertussis lipooligosaccharide (BpLOS) induce caspase-11 (Casp11) and guanylate-binding proteins (GBPs) dependent non-canonical inflammasome activation. Our results elucidate the mechanism by which BpOMVs trigger one central pathway of the innate response activation that is expected to skew the adaptive immune response elicited by BpOMVs vaccination.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154545
Elizagaray, Maia Lina; Gomes, Marco Túlio R.; Guimaraes, Erika S.; Rumbo, Martín; Hozbor, Daniela Flavia; et al.; Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis; Frontiers Media; Frontiers in Immunology; 11; 20-8-2020; 1-13
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154545
identifier_str_mv Elizagaray, Maia Lina; Gomes, Marco Túlio R.; Guimaraes, Erika S.; Rumbo, Martín; Hozbor, Daniela Flavia; et al.; Canonical and non-canonical inflammasome activation by outer membrane vesicles derived from Bordetella pertussis; Frontiers Media; Frontiers in Immunology; 11; 20-8-2020; 1-13
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fimmu.2020.01879/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2020.01879
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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