Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats

Autores
Ibañez, Alejandro Martín; Espejo, María Sofía; Zavala, Maite Raquel; Villa Abrille, María Celeste; Lofeudo, Juan Manuel; Aiello, Ernesto Alejandro; De Giusti, Verónica Celeste
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: It is well known that after menopause women are exposed to a greater cardiovascular risk, but the intracellular modifications are not properly described. The sodium/proton exchanger (NHE) and the sodium/bicarbonate cotransporter (NBC) regulate the intracellular pH and, indirectly, the intracellular sodium concentration ([Na+]). There are 2 isoforms of NBC in the heart: the electrogenic (1Na+/2HCO 3 ; NBCe1) and the electroneutral (1Na+/1HCO 3 ; NBCn1). Because NHE and NBCn1 hyperactivity as well as the NBCe1 decreased activity have been associated with several cardiovascular pathologies, the aim of this study was to investigate the potential alterations of the alkalinizing transporters during the postmenopausal period. Methods and Results: Three-month ovariectomized rats (OVX) were used. The NHE activity and protein expression are significantly increased in OVX. The NBCe1 activity is diminished, and the NBCn1 activity becomes predominant in OVX rats. p-Akt levels showed a significant diminution in OVX. Finally, NHE activity in platelets from OVX rats is also higher in comparison to sham rats, resulting in a potential biomarker of cardiovascular diseases. Conclusions: Our results demonstrated for the first time that in the cardiac ventricular myocytes of OVX rats NHE and NBC isoforms are altered, probably because of the decreased level of p-Akt, compromising the ionic intracellular homeostasis. (J Am Heart Assoc. 2019;8:e011066. DOI: 10.1161/JAHA.118.011066.)
Centro de Investigaciones Cardiovasculares
Materia
Ciencias Médicas
Alcalinizing transporters
Cardiac myocyte
Cardiac remodeling
Cardiovascular pathophysiology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/102984
Acceder
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network_name_str SEDICI (UNLP)
spelling Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized RatsIbañez, Alejandro MartínEspejo, María SofíaZavala, Maite RaquelVilla Abrille, María CelesteLofeudo, Juan ManuelAiello, Ernesto AlejandroDe Giusti, Verónica CelesteCiencias MédicasAlcalinizing transportersCardiac myocyteCardiac remodelingCardiovascular pathophysiologyBackground: It is well known that after menopause women are exposed to a greater cardiovascular risk, but the intracellular modifications are not properly described. The sodium/proton exchanger (NHE) and the sodium/bicarbonate cotransporter (NBC) regulate the intracellular pH and, indirectly, the intracellular sodium concentration ([Na+]). There are 2 isoforms of NBC in the heart: the electrogenic (1Na+/2HCO 3 ; NBCe1) and the electroneutral (1Na+/1HCO 3 ; NBCn1). Because NHE and NBCn1 hyperactivity as well as the NBCe1 decreased activity have been associated with several cardiovascular pathologies, the aim of this study was to investigate the potential alterations of the alkalinizing transporters during the postmenopausal period. Methods and Results: Three-month ovariectomized rats (OVX) were used. The NHE activity and protein expression are significantly increased in OVX. The NBCe1 activity is diminished, and the NBCn1 activity becomes predominant in OVX rats. p-Akt levels showed a significant diminution in OVX. Finally, NHE activity in platelets from OVX rats is also higher in comparison to sham rats, resulting in a potential biomarker of cardiovascular diseases. Conclusions: Our results demonstrated for the first time that in the cardiac ventricular myocytes of OVX rats NHE and NBC isoforms are altered, probably because of the decreased level of p-Akt, compromising the ionic intracellular homeostasis. (J Am Heart Assoc. 2019;8:e011066. DOI: 10.1161/JAHA.118.011066.)Centro de Investigaciones Cardiovasculares2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/102984enginfo:eu-repo/semantics/altIdentifier/issn/2047-9980info:eu-repo/semantics/altIdentifier/doi/10.1161/jaha.118.011066info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:54:29Zoai:sedici.unlp.edu.ar:10915/102984Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:54:30.096SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
spellingShingle Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
Ibañez, Alejandro Martín
Ciencias Médicas
Alcalinizing transporters
Cardiac myocyte
Cardiac remodeling
Cardiovascular pathophysiology
title_short Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_full Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_fullStr Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_full_unstemmed Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_sort Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
dc.creator.none.fl_str_mv Ibañez, Alejandro Martín
Espejo, María Sofía
Zavala, Maite Raquel
Villa Abrille, María Celeste
Lofeudo, Juan Manuel
Aiello, Ernesto Alejandro
De Giusti, Verónica Celeste
author Ibañez, Alejandro Martín
author_facet Ibañez, Alejandro Martín
Espejo, María Sofía
Zavala, Maite Raquel
Villa Abrille, María Celeste
Lofeudo, Juan Manuel
Aiello, Ernesto Alejandro
De Giusti, Verónica Celeste
author_role author
author2 Espejo, María Sofía
Zavala, Maite Raquel
Villa Abrille, María Celeste
Lofeudo, Juan Manuel
Aiello, Ernesto Alejandro
De Giusti, Verónica Celeste
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Alcalinizing transporters
Cardiac myocyte
Cardiac remodeling
Cardiovascular pathophysiology
topic Ciencias Médicas
Alcalinizing transporters
Cardiac myocyte
Cardiac remodeling
Cardiovascular pathophysiology
dc.description.none.fl_txt_mv Background: It is well known that after menopause women are exposed to a greater cardiovascular risk, but the intracellular modifications are not properly described. The sodium/proton exchanger (NHE) and the sodium/bicarbonate cotransporter (NBC) regulate the intracellular pH and, indirectly, the intracellular sodium concentration ([Na+]). There are 2 isoforms of NBC in the heart: the electrogenic (1Na+/2HCO 3 ; NBCe1) and the electroneutral (1Na+/1HCO 3 ; NBCn1). Because NHE and NBCn1 hyperactivity as well as the NBCe1 decreased activity have been associated with several cardiovascular pathologies, the aim of this study was to investigate the potential alterations of the alkalinizing transporters during the postmenopausal period. Methods and Results: Three-month ovariectomized rats (OVX) were used. The NHE activity and protein expression are significantly increased in OVX. The NBCe1 activity is diminished, and the NBCn1 activity becomes predominant in OVX rats. p-Akt levels showed a significant diminution in OVX. Finally, NHE activity in platelets from OVX rats is also higher in comparison to sham rats, resulting in a potential biomarker of cardiovascular diseases. Conclusions: Our results demonstrated for the first time that in the cardiac ventricular myocytes of OVX rats NHE and NBC isoforms are altered, probably because of the decreased level of p-Akt, compromising the ionic intracellular homeostasis. (J Am Heart Assoc. 2019;8:e011066. DOI: 10.1161/JAHA.118.011066.)
Centro de Investigaciones Cardiovasculares
description Background: It is well known that after menopause women are exposed to a greater cardiovascular risk, but the intracellular modifications are not properly described. The sodium/proton exchanger (NHE) and the sodium/bicarbonate cotransporter (NBC) regulate the intracellular pH and, indirectly, the intracellular sodium concentration ([Na+]). There are 2 isoforms of NBC in the heart: the electrogenic (1Na+/2HCO 3 ; NBCe1) and the electroneutral (1Na+/1HCO 3 ; NBCn1). Because NHE and NBCn1 hyperactivity as well as the NBCe1 decreased activity have been associated with several cardiovascular pathologies, the aim of this study was to investigate the potential alterations of the alkalinizing transporters during the postmenopausal period. Methods and Results: Three-month ovariectomized rats (OVX) were used. The NHE activity and protein expression are significantly increased in OVX. The NBCe1 activity is diminished, and the NBCn1 activity becomes predominant in OVX rats. p-Akt levels showed a significant diminution in OVX. Finally, NHE activity in platelets from OVX rats is also higher in comparison to sham rats, resulting in a potential biomarker of cardiovascular diseases. Conclusions: Our results demonstrated for the first time that in the cardiac ventricular myocytes of OVX rats NHE and NBC isoforms are altered, probably because of the decreased level of p-Akt, compromising the ionic intracellular homeostasis. (J Am Heart Assoc. 2019;8:e011066. DOI: 10.1161/JAHA.118.011066.)
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/102984
url http://sedici.unlp.edu.ar/handle/10915/102984
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2047-9980
info:eu-repo/semantics/altIdentifier/doi/10.1161/jaha.118.011066
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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