Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes

Autores
Marambio, Paola; Toro, Barbra; Sanhueza, Carlos; Troncoso, Rodrigo; Parra, Valentina; Verdejo, Hugo; García, Lorena; Quiroga, Clara; Munafó, Daniela B.; Díaz Elizondo, Jessica; Bravo, Roberto; González, María Julieta; Diaz Araya, Guilermo; Pedrozo, Zully; Chiong, Mario; Colombo, Maria Isabel; Lavandero, Sergio
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aggresomes are dynamic structures formed when the ubiquitin-proteasome system is overwhelmed with aggregation-prone proteins. In this process, small protein aggregates are actively transported towards the microtubule-organizing center. A functional role for autophagy in the clearance of aggresomes has also been proposed. In the present work we investigated the molecular mechanisms involved on aggresome formation in cultured rat cardiac myocytes exposed to glucose deprivation. Confocal microscopy showed that small aggregates of polyubiquitinated proteins were formed in cells exposed to glucose deprivation for 6 h. However, at longer times (18 h), aggregates formed large perinuclear inclusions (aggresomes) which colocalized with gamma-tubulin (a microtubule-organizing center marker) and Hsp70. The microtubule disrupting agent vinblastine prevented the formation of these inclusions. Both small aggregates and aggresomes colocalized with autophagy markers such as GFP-LC3 and Rab24. Glucose deprivation stimulates reactive oxygen species (ROS) production and decreases intracellular glutathione levels. ROS inhibition by N-acetylcysteine or by the adenoviral overexpression of catalase or superoxide dismutase disrupted aggresome formation and autophagy induced by glucose deprivation. In conclusion, glucose deprivation induces oxidative stress which is associated with aggresome formation and activation of autophagy in cultured cardiac myocytes.
Fil: Marambio, Paola. Universidad de Chile; Chile
Fil: Toro, Barbra. Universidad de Chile; Chile
Fil: Sanhueza, Carlos. Universidad de Chile; Chile
Fil: Troncoso, Rodrigo. Universidad de Chile; Chile
Fil: Parra, Valentina. Universidad de Chile; Chile
Fil: Verdejo, Hugo. Universidad de Chile; Chile
Fil: García, Lorena. Universidad de Chile; Chile
Fil: Quiroga, Clara. Universidad de Chile; Chile
Fil: Munafó, Daniela B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Díaz Elizondo, Jessica. Universidad de Chile; Chile
Fil: Bravo, Roberto. Universidad de Chile; Chile
Fil: González, María Julieta. Universidad de Chile; Chile
Fil: Diaz Araya, Guilermo. Universidad de Chile; Chile
Fil: Pedrozo, Zully. Universidad de Chile; Chile
Fil: Chiong, Mario. Universidad de Chile; Chile
Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Lavandero, Sergio. Universidad de Chile; Chile
Materia
autophagy
aggresomes
cardiac myocytes
glucose deprivation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247628
Acceder
id CONICETDig_0d4be45a1dd80c7ed2a97504aa0127cd
oai_identifier_str oai:ri.conicet.gov.ar:11336/247628
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytesMarambio, PaolaToro, BarbraSanhueza, CarlosTroncoso, RodrigoParra, ValentinaVerdejo, HugoGarcía, LorenaQuiroga, ClaraMunafó, Daniela B.Díaz Elizondo, JessicaBravo, RobertoGonzález, María JulietaDiaz Araya, GuilermoPedrozo, ZullyChiong, MarioColombo, Maria IsabelLavandero, Sergioautophagyaggresomescardiac myocytesglucose deprivationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Aggresomes are dynamic structures formed when the ubiquitin-proteasome system is overwhelmed with aggregation-prone proteins. In this process, small protein aggregates are actively transported towards the microtubule-organizing center. A functional role for autophagy in the clearance of aggresomes has also been proposed. In the present work we investigated the molecular mechanisms involved on aggresome formation in cultured rat cardiac myocytes exposed to glucose deprivation. Confocal microscopy showed that small aggregates of polyubiquitinated proteins were formed in cells exposed to glucose deprivation for 6 h. However, at longer times (18 h), aggregates formed large perinuclear inclusions (aggresomes) which colocalized with gamma-tubulin (a microtubule-organizing center marker) and Hsp70. The microtubule disrupting agent vinblastine prevented the formation of these inclusions. Both small aggregates and aggresomes colocalized with autophagy markers such as GFP-LC3 and Rab24. Glucose deprivation stimulates reactive oxygen species (ROS) production and decreases intracellular glutathione levels. ROS inhibition by N-acetylcysteine or by the adenoviral overexpression of catalase or superoxide dismutase disrupted aggresome formation and autophagy induced by glucose deprivation. In conclusion, glucose deprivation induces oxidative stress which is associated with aggresome formation and activation of autophagy in cultured cardiac myocytes.Fil: Marambio, Paola. Universidad de Chile; ChileFil: Toro, Barbra. Universidad de Chile; ChileFil: Sanhueza, Carlos. Universidad de Chile; ChileFil: Troncoso, Rodrigo. Universidad de Chile; ChileFil: Parra, Valentina. Universidad de Chile; ChileFil: Verdejo, Hugo. Universidad de Chile; ChileFil: García, Lorena. Universidad de Chile; ChileFil: Quiroga, Clara. Universidad de Chile; ChileFil: Munafó, Daniela B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Díaz Elizondo, Jessica. Universidad de Chile; ChileFil: Bravo, Roberto. Universidad de Chile; ChileFil: González, María Julieta. Universidad de Chile; ChileFil: Diaz Araya, Guilermo. Universidad de Chile; ChileFil: Pedrozo, Zully. Universidad de Chile; ChileFil: Chiong, Mario. Universidad de Chile; ChileFil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Lavandero, Sergio. Universidad de Chile; ChileElsevier Science2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247628Marambio, Paola; Toro, Barbra; Sanhueza, Carlos; Troncoso, Rodrigo; Parra, Valentina; et al.; Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes; Elsevier Science; Biochimica et Biophysica Acta - Molecular Basis of Disease; 1802; 6; 6-2010; 509-5180925-4439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S092544391000044Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbadis.2010.02.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:53Zoai:ri.conicet.gov.ar:11336/247628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:54.288CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
title Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
spellingShingle Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
Marambio, Paola
autophagy
aggresomes
cardiac myocytes
glucose deprivation
title_short Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
title_full Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
title_fullStr Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
title_full_unstemmed Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
title_sort Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
dc.creator.none.fl_str_mv Marambio, Paola
Toro, Barbra
Sanhueza, Carlos
Troncoso, Rodrigo
Parra, Valentina
Verdejo, Hugo
García, Lorena
Quiroga, Clara
Munafó, Daniela B.
Díaz Elizondo, Jessica
Bravo, Roberto
González, María Julieta
Diaz Araya, Guilermo
Pedrozo, Zully
Chiong, Mario
Colombo, Maria Isabel
Lavandero, Sergio
author Marambio, Paola
author_facet Marambio, Paola
Toro, Barbra
Sanhueza, Carlos
Troncoso, Rodrigo
Parra, Valentina
Verdejo, Hugo
García, Lorena
Quiroga, Clara
Munafó, Daniela B.
Díaz Elizondo, Jessica
Bravo, Roberto
González, María Julieta
Diaz Araya, Guilermo
Pedrozo, Zully
Chiong, Mario
Colombo, Maria Isabel
Lavandero, Sergio
author_role author
author2 Toro, Barbra
Sanhueza, Carlos
Troncoso, Rodrigo
Parra, Valentina
Verdejo, Hugo
García, Lorena
Quiroga, Clara
Munafó, Daniela B.
Díaz Elizondo, Jessica
Bravo, Roberto
González, María Julieta
Diaz Araya, Guilermo
Pedrozo, Zully
Chiong, Mario
Colombo, Maria Isabel
Lavandero, Sergio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv autophagy
aggresomes
cardiac myocytes
glucose deprivation
topic autophagy
aggresomes
cardiac myocytes
glucose deprivation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aggresomes are dynamic structures formed when the ubiquitin-proteasome system is overwhelmed with aggregation-prone proteins. In this process, small protein aggregates are actively transported towards the microtubule-organizing center. A functional role for autophagy in the clearance of aggresomes has also been proposed. In the present work we investigated the molecular mechanisms involved on aggresome formation in cultured rat cardiac myocytes exposed to glucose deprivation. Confocal microscopy showed that small aggregates of polyubiquitinated proteins were formed in cells exposed to glucose deprivation for 6 h. However, at longer times (18 h), aggregates formed large perinuclear inclusions (aggresomes) which colocalized with gamma-tubulin (a microtubule-organizing center marker) and Hsp70. The microtubule disrupting agent vinblastine prevented the formation of these inclusions. Both small aggregates and aggresomes colocalized with autophagy markers such as GFP-LC3 and Rab24. Glucose deprivation stimulates reactive oxygen species (ROS) production and decreases intracellular glutathione levels. ROS inhibition by N-acetylcysteine or by the adenoviral overexpression of catalase or superoxide dismutase disrupted aggresome formation and autophagy induced by glucose deprivation. In conclusion, glucose deprivation induces oxidative stress which is associated with aggresome formation and activation of autophagy in cultured cardiac myocytes.
Fil: Marambio, Paola. Universidad de Chile; Chile
Fil: Toro, Barbra. Universidad de Chile; Chile
Fil: Sanhueza, Carlos. Universidad de Chile; Chile
Fil: Troncoso, Rodrigo. Universidad de Chile; Chile
Fil: Parra, Valentina. Universidad de Chile; Chile
Fil: Verdejo, Hugo. Universidad de Chile; Chile
Fil: García, Lorena. Universidad de Chile; Chile
Fil: Quiroga, Clara. Universidad de Chile; Chile
Fil: Munafó, Daniela B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Díaz Elizondo, Jessica. Universidad de Chile; Chile
Fil: Bravo, Roberto. Universidad de Chile; Chile
Fil: González, María Julieta. Universidad de Chile; Chile
Fil: Diaz Araya, Guilermo. Universidad de Chile; Chile
Fil: Pedrozo, Zully. Universidad de Chile; Chile
Fil: Chiong, Mario. Universidad de Chile; Chile
Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Lavandero, Sergio. Universidad de Chile; Chile
description Aggresomes are dynamic structures formed when the ubiquitin-proteasome system is overwhelmed with aggregation-prone proteins. In this process, small protein aggregates are actively transported towards the microtubule-organizing center. A functional role for autophagy in the clearance of aggresomes has also been proposed. In the present work we investigated the molecular mechanisms involved on aggresome formation in cultured rat cardiac myocytes exposed to glucose deprivation. Confocal microscopy showed that small aggregates of polyubiquitinated proteins were formed in cells exposed to glucose deprivation for 6 h. However, at longer times (18 h), aggregates formed large perinuclear inclusions (aggresomes) which colocalized with gamma-tubulin (a microtubule-organizing center marker) and Hsp70. The microtubule disrupting agent vinblastine prevented the formation of these inclusions. Both small aggregates and aggresomes colocalized with autophagy markers such as GFP-LC3 and Rab24. Glucose deprivation stimulates reactive oxygen species (ROS) production and decreases intracellular glutathione levels. ROS inhibition by N-acetylcysteine or by the adenoviral overexpression of catalase or superoxide dismutase disrupted aggresome formation and autophagy induced by glucose deprivation. In conclusion, glucose deprivation induces oxidative stress which is associated with aggresome formation and activation of autophagy in cultured cardiac myocytes.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247628
Marambio, Paola; Toro, Barbra; Sanhueza, Carlos; Troncoso, Rodrigo; Parra, Valentina; et al.; Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes; Elsevier Science; Biochimica et Biophysica Acta - Molecular Basis of Disease; 1802; 6; 6-2010; 509-518
0925-4439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247628
identifier_str_mv Marambio, Paola; Toro, Barbra; Sanhueza, Carlos; Troncoso, Rodrigo; Parra, Valentina; et al.; Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes; Elsevier Science; Biochimica et Biophysica Acta - Molecular Basis of Disease; 1802; 6; 6-2010; 509-518
0925-4439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S092544391000044X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbadis.2010.02.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269724306571264
score 13.13397

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