Preliminary study of alterations in human red blood cells by irradiation with high energy photons

Autores
Riquelme, Bibiana Doris; Estrada, Ezequiel; Castellini, Horacio V.; Acosta, Andrea; Chinelatto, Alejandro; Tack, Ivan; Borraz, Javier; Di Tullio, Liliana; Galassi, Mariel Elisa
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction: Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008). In a recent work (AlZahrani K. et al., 2017), nanoestructural changes in the RBC membrane at different doses of gamma irradiation were observed using atomic force microscopy. The images shown that the roughness of the cell membrane increased dramatically with increasing doses, affecting their biophysical properties. However, more research is needed to understand the effects of gamma irradiation on the mechanical and adhesion properties of RBC. For this reason, in the present work we set out to measure the mechanical and aggregation parameters of human red blood cells exposed to gamma photons in different doses in order to determine the possible alterations due to radiation.
Fil: Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.
Fil: Fil: Estrada, Ezequiel. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.
Fil: Fil: Castellini, Horacio V. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.
Fil: Fil: Acosta, Andrea. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Chinelatto, Alejandro. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Tack, Ivan, Ivan. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Borraz, Javier. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Di Tullio, Liliana. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Galassi, Mariel Elisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.
Fil: Fil: Galassi, Mariel Elisa. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.
Materia
Red Blood Cells
Erythrocytes
High Energy Photons
Radiation Effects
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
Repositorio
RepHipUNR (UNR)
Institución
Universidad Nacional de Rosario
OAI Identificador
oai:rephip.unr.edu.ar:2133/17140
Acceder
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oai_identifier_str oai:rephip.unr.edu.ar:2133/17140
network_acronym_str RepHipUNR
repository_id_str 1550
network_name_str RepHipUNR (UNR)
spelling Preliminary study of alterations in human red blood cells by irradiation with high energy photonsRiquelme, Bibiana DorisEstrada, EzequielCastellini, Horacio V.Acosta, AndreaChinelatto, AlejandroTack, IvanBorraz, JavierDi Tullio, LilianaGalassi, Mariel ElisaRed Blood CellsErythrocytesHigh Energy PhotonsRadiation EffectsIntroduction: Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008). In a recent work (AlZahrani K. et al., 2017), nanoestructural changes in the RBC membrane at different doses of gamma irradiation were observed using atomic force microscopy. The images shown that the roughness of the cell membrane increased dramatically with increasing doses, affecting their biophysical properties. However, more research is needed to understand the effects of gamma irradiation on the mechanical and adhesion properties of RBC. For this reason, in the present work we set out to measure the mechanical and aggregation parameters of human red blood cells exposed to gamma photons in different doses in order to determine the possible alterations due to radiation.Fil: Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.Fil: Fil: Estrada, Ezequiel. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.Fil: Fil: Castellini, Horacio V. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.Fil: Fil: Acosta, Andrea. Centro Regional de Hemoterapia de Santa Fe; Argentina.Fil: Fil: Chinelatto, Alejandro. Centro Regional de Hemoterapia de Santa Fe; Argentina.Fil: Fil: Tack, Ivan, Ivan. Centro Regional de Hemoterapia de Santa Fe; Argentina.Fil: Fil: Borraz, Javier. Centro Regional de Hemoterapia de Santa Fe; Argentina.Fil: Fil: Di Tullio, Liliana. Centro Regional de Hemoterapia de Santa Fe; Argentina.Fil: Fil: Galassi, Mariel Elisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.Fil: Fil: Galassi, Mariel Elisa. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.BlucherD'Arrigo, Mabel. Colaboración en preparación de muestras biológicasCastellani, Daniel. Colaboración en la fabricación del dispositivo adaptador de muestra.Sociedade Brasileira de Biofísica2019info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/2133/17140urn:issn: 2526-6071enghttps://www.proceedings.blucher.com.br/article-details/preliminary-study-of-alterations-in-human-red-blood-cells-by-irradiation-with-high-energy-photons-30605http://dx.doi.org/10.5151/biofisica2019-30info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)https://creativecommons.org/licenses/by-nc-nd/4.0/deed.esLicencia RepHipreponame:RepHipUNR (UNR)instname:Universidad Nacional de Rosario2025-09-04T09:46:04Zoai:rephip.unr.edu.ar:2133/17140instacron:UNRInstitucionalhttps://rephip.unr.edu.ar/Universidad públicaNo correspondehttps://rephip.unr.edu.ar/oai/requestrephip@unr.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:15502025-09-04 09:46:06.055RepHipUNR (UNR) - Universidad Nacional de Rosariofalse
dc.title.none.fl_str_mv Preliminary study of alterations in human red blood cells by irradiation with high energy photons
title Preliminary study of alterations in human red blood cells by irradiation with high energy photons
spellingShingle Preliminary study of alterations in human red blood cells by irradiation with high energy photons
Riquelme, Bibiana Doris
Red Blood Cells
Erythrocytes
High Energy Photons
Radiation Effects
title_short Preliminary study of alterations in human red blood cells by irradiation with high energy photons
title_full Preliminary study of alterations in human red blood cells by irradiation with high energy photons
title_fullStr Preliminary study of alterations in human red blood cells by irradiation with high energy photons
title_full_unstemmed Preliminary study of alterations in human red blood cells by irradiation with high energy photons
title_sort Preliminary study of alterations in human red blood cells by irradiation with high energy photons
dc.creator.none.fl_str_mv Riquelme, Bibiana Doris
Estrada, Ezequiel
Castellini, Horacio V.
Acosta, Andrea
Chinelatto, Alejandro
Tack, Ivan
Borraz, Javier
Di Tullio, Liliana
Galassi, Mariel Elisa
author Riquelme, Bibiana Doris
author_facet Riquelme, Bibiana Doris
Estrada, Ezequiel
Castellini, Horacio V.
Acosta, Andrea
Chinelatto, Alejandro
Tack, Ivan
Borraz, Javier
Di Tullio, Liliana
Galassi, Mariel Elisa
author_role author
author2 Estrada, Ezequiel
Castellini, Horacio V.
Acosta, Andrea
Chinelatto, Alejandro
Tack, Ivan
Borraz, Javier
Di Tullio, Liliana
Galassi, Mariel Elisa
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv D'Arrigo, Mabel. Colaboración en preparación de muestras biológicas
Castellani, Daniel. Colaboración en la fabricación del dispositivo adaptador de muestra.
Sociedade Brasileira de Biofísica
dc.subject.none.fl_str_mv Red Blood Cells
Erythrocytes
High Energy Photons
Radiation Effects
topic Red Blood Cells
Erythrocytes
High Energy Photons
Radiation Effects
dc.description.none.fl_txt_mv Introduction: Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008). In a recent work (AlZahrani K. et al., 2017), nanoestructural changes in the RBC membrane at different doses of gamma irradiation were observed using atomic force microscopy. The images shown that the roughness of the cell membrane increased dramatically with increasing doses, affecting their biophysical properties. However, more research is needed to understand the effects of gamma irradiation on the mechanical and adhesion properties of RBC. For this reason, in the present work we set out to measure the mechanical and aggregation parameters of human red blood cells exposed to gamma photons in different doses in order to determine the possible alterations due to radiation.
Fil: Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.
Fil: Fil: Estrada, Ezequiel. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.
Fil: Fil: Castellini, Horacio V. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.
Fil: Fil: Acosta, Andrea. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Chinelatto, Alejandro. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Tack, Ivan, Ivan. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Borraz, Javier. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Di Tullio, Liliana. Centro Regional de Hemoterapia de Santa Fe; Argentina.
Fil: Fil: Galassi, Mariel Elisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.
Fil: Fil: Galassi, Mariel Elisa. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.
description Introduction: Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008). In a recent work (AlZahrani K. et al., 2017), nanoestructural changes in the RBC membrane at different doses of gamma irradiation were observed using atomic force microscopy. The images shown that the roughness of the cell membrane increased dramatically with increasing doses, affecting their biophysical properties. However, more research is needed to understand the effects of gamma irradiation on the mechanical and adhesion properties of RBC. For this reason, in the present work we set out to measure the mechanical and aggregation parameters of human red blood cells exposed to gamma photons in different doses in order to determine the possible alterations due to radiation.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject


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identifier_str_mv urn:issn: 2526-6071
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.proceedings.blucher.com.br/article-details/preliminary-study-of-alterations-in-human-red-blood-cells-by-irradiation-with-high-energy-photons-30605
http://dx.doi.org/10.5151/biofisica2019-30
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rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
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Licencia RepHip
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dc.publisher.none.fl_str_mv Blucher
publisher.none.fl_str_mv Blucher
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