Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum

Autores
Podio, Maricel; Felitti, Silvina Andrea; Siena, Lorena Adelina; Delgado Benarroch, Luciana; Mancini, Micaela; Seijo, José Guillermo; González, Ana María; Pessino, Silvina Claudia; Ortíz, Juan Pablo Amelio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Delgado Benarroch, Luciana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mancini, Micaela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
The SOMATIC EMBRYOGENESIS RECEPTOR- LIKE KINASE (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression pat- terns of SERK genes in apomictic and sexual genotypes of Paspalum notatum. A conserved 200-bp gene fragment was amplified from genomic DNA with heterologous primers, and used to initiate a chromosomal walking strategy for cloning the complete sequence. This procedure allowed the isolation of two members of the P. notatum SERK family; PnSERK1, which is similar to PpSERK1, and PnSERK2, which is similar to ZmSERK2 and AtSERK1. Phylogenetic analyses indicated that PnSERK1 and PnSERK2 represent paralogous sequences. Southern-blot hybridization indi- cated the presence of at least three copies of SERK genes in the species. qRT-PCR analyses revealed that PnSERK2 was expressed at significantly higher levels than PnSERK1 in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that PnSERK2 displayed a spatially and chronologically altered expression pattern in reproductive organs of the apom- ictic genotype with respect to the sexual one. PnSERK2 is expressed in nucellar cells of the apomictic genotype at meiosis, but only in the megaspore mother cell in the sexual genotype. Therefore, apomixis onset in P. notatum seems to be correlated with the expression of PnSERK2 in nucellar tissue.
Fuente
Plant Molecular Biology, 2014, vol. 84, no. 4, p. 479-495.
Materia
Paspalum notatum
Apomixis
Apospory
SERK
qRT-PCR
In situ hybridization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/47988
Acceder
id RIUNNE_a74e5a9211b615da965d2b813106ef22
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/47988
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatumPodio, MaricelFelitti, Silvina AndreaSiena, Lorena AdelinaDelgado Benarroch, LucianaMancini, MicaelaSeijo, José GuillermoGonzález, Ana MaríaPessino, Silvina ClaudiaOrtíz, Juan Pablo AmelioPaspalum notatumApomixisAposporySERKqRT-PCRIn situ hybridizationFil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Delgado Benarroch, Luciana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Mancini, Micaela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.The SOMATIC EMBRYOGENESIS RECEPTOR- LIKE KINASE (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression pat- terns of SERK genes in apomictic and sexual genotypes of Paspalum notatum. A conserved 200-bp gene fragment was amplified from genomic DNA with heterologous primers, and used to initiate a chromosomal walking strategy for cloning the complete sequence. This procedure allowed the isolation of two members of the P. notatum SERK family; PnSERK1, which is similar to PpSERK1, and PnSERK2, which is similar to ZmSERK2 and AtSERK1. Phylogenetic analyses indicated that PnSERK1 and PnSERK2 represent paralogous sequences. Southern-blot hybridization indi- cated the presence of at least three copies of SERK genes in the species. qRT-PCR analyses revealed that PnSERK2 was expressed at significantly higher levels than PnSERK1 in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that PnSERK2 displayed a spatially and chronologically altered expression pattern in reproductive organs of the apom- ictic genotype with respect to the sexual one. PnSERK2 is expressed in nucellar cells of the apomictic genotype at meiosis, but only in the megaspore mother cell in the sexual genotype. Therefore, apomixis onset in P. notatum seems to be correlated with the expression of PnSERK2 in nucellar tissue.Plant Molecular Biology2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfPodio, Maricel, et al., 2014. Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum. Springer. Ámsterdam: Plant Molecular Biology, vol. 84, no. 4, p. 479-495. ISSN-e 1573-5028.0167-4412http://repositorio.unne.edu.ar/handle/123456789/47988Plant Molecular Biology, 2014, vol. 84, no. 4, p. 479-495.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://link.springer.com/article/10.1007/s11103-013-0146-9https://pubmed.ncbi.nlm.nih.gov/24146222/info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-10-16T10:07:13Zoai:repositorio.unne.edu.ar:123456789/47988instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-10-16 10:07:13.338Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
spellingShingle Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
Podio, Maricel
Paspalum notatum
Apomixis
Apospory
SERK
qRT-PCR
In situ hybridization
title_short Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_full Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_fullStr Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_full_unstemmed Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_sort Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
dc.creator.none.fl_str_mv Podio, Maricel
Felitti, Silvina Andrea
Siena, Lorena Adelina
Delgado Benarroch, Luciana
Mancini, Micaela
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author Podio, Maricel
author_facet Podio, Maricel
Felitti, Silvina Andrea
Siena, Lorena Adelina
Delgado Benarroch, Luciana
Mancini, Micaela
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author_role author
author2 Felitti, Silvina Andrea
Siena, Lorena Adelina
Delgado Benarroch, Luciana
Mancini, Micaela
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Paspalum notatum
Apomixis
Apospory
SERK
qRT-PCR
In situ hybridization
topic Paspalum notatum
Apomixis
Apospory
SERK
qRT-PCR
In situ hybridization
dc.description.none.fl_txt_mv Fil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Delgado Benarroch, Luciana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mancini, Micaela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
The SOMATIC EMBRYOGENESIS RECEPTOR- LIKE KINASE (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression pat- terns of SERK genes in apomictic and sexual genotypes of Paspalum notatum. A conserved 200-bp gene fragment was amplified from genomic DNA with heterologous primers, and used to initiate a chromosomal walking strategy for cloning the complete sequence. This procedure allowed the isolation of two members of the P. notatum SERK family; PnSERK1, which is similar to PpSERK1, and PnSERK2, which is similar to ZmSERK2 and AtSERK1. Phylogenetic analyses indicated that PnSERK1 and PnSERK2 represent paralogous sequences. Southern-blot hybridization indi- cated the presence of at least three copies of SERK genes in the species. qRT-PCR analyses revealed that PnSERK2 was expressed at significantly higher levels than PnSERK1 in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that PnSERK2 displayed a spatially and chronologically altered expression pattern in reproductive organs of the apom- ictic genotype with respect to the sexual one. PnSERK2 is expressed in nucellar cells of the apomictic genotype at meiosis, but only in the megaspore mother cell in the sexual genotype. Therefore, apomixis onset in P. notatum seems to be correlated with the expression of PnSERK2 in nucellar tissue.
description Fil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Podio, Maricel, et al., 2014. Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum. Springer. Ámsterdam: Plant Molecular Biology, vol. 84, no. 4, p. 479-495. ISSN-e 1573-5028.
0167-4412
http://repositorio.unne.edu.ar/handle/123456789/47988
identifier_str_mv Podio, Maricel, et al., 2014. Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum. Springer. Ámsterdam: Plant Molecular Biology, vol. 84, no. 4, p. 479-495. ISSN-e 1573-5028.
0167-4412
url http://repositorio.unne.edu.ar/handle/123456789/47988
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://link.springer.com/article/10.1007/s11103-013-0146-9
https://pubmed.ncbi.nlm.nih.gov/24146222/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Plant Molecular Biology
publisher.none.fl_str_mv Plant Molecular Biology
dc.source.none.fl_str_mv Plant Molecular Biology, 2014, vol. 84, no. 4, p. 479-495.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.712165

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