The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes

Autores
Bocchini, Marika; Galla, Giulio; Pupilli, Fulvio; Bellucci, Michele; Barcaccia, Gianni; Ortiz, Juan Pablo Amelio; Pessino, Silvina Claudia; Albertini, Emidio
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apomixis (asexual reproduction through seeds) is considered a deviation of the sexual reproductive pathway leading to the development of clonal progenies genetically identical to the mother plant. Here we used the Methylation-Sensitive Amplification Polymorphism (MSAP) technique to characterize cytosine methylation patterns occurring in florets of sexual and aposporous Paspalum notatum genotypes, in order to identify epigenetically-controlled genes putatively involved in apomixis development. From twelve polymorphic MSAP-derived sequences, one (PN-6.6, later renamed PN-SCD1) was selected due to its relevant annotation and differential representation in apomictic and sexual floral transcriptome libraries. PN-SCD1 encodes the DENN domain/WD repeat-containing protein SCD1, which interacts with RAB GTPases- and/or MAPKs to promote specialized cell division, functions in clathrin-mediated membrane transport and acts as potential substrate receptor of CUL4 E3 ubiquitin ligases. Quantitative RT-PCR and comparative RNAseq analyses of laser microdissected nucellar cells confirmed PN-SCD1 upregulation in florets of apomictic plants and revealed that overexpression takes place just before the onset of apospory initials. Moreover, we found that several SCD1 molecular partners are expressed in P. notatum florets and upregulated in apomictic plants. Our results disclosed a specific vesicle trafficking molecular pathway epigenetically modulated during apomixis.
Fil: Bocchini, Marika. Università di Perugia; Italia
Fil: Galla, Giulio. Università di Padova; Italia
Fil: Pupilli, Fulvio. Consiglio Nazionale delle Ricerche; Italia
Fil: Bellucci, Michele. Consiglio Nazionale delle Ricerche; Italia
Fil: Barcaccia, Gianni. Università di Padova; Italia
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Albertini, Emidio. Università di Perugia; Italia
Materia
APOMIXIS
PASPALUM NOTATUM
METHYLATION
SCD1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91961
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypesBocchini, MarikaGalla, GiulioPupilli, FulvioBellucci, MicheleBarcaccia, GianniOrtiz, Juan Pablo AmelioPessino, Silvina ClaudiaAlbertini, EmidioAPOMIXISPASPALUM NOTATUMMETHYLATIONSCD1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Apomixis (asexual reproduction through seeds) is considered a deviation of the sexual reproductive pathway leading to the development of clonal progenies genetically identical to the mother plant. Here we used the Methylation-Sensitive Amplification Polymorphism (MSAP) technique to characterize cytosine methylation patterns occurring in florets of sexual and aposporous Paspalum notatum genotypes, in order to identify epigenetically-controlled genes putatively involved in apomixis development. From twelve polymorphic MSAP-derived sequences, one (PN-6.6, later renamed PN-SCD1) was selected due to its relevant annotation and differential representation in apomictic and sexual floral transcriptome libraries. PN-SCD1 encodes the DENN domain/WD repeat-containing protein SCD1, which interacts with RAB GTPases- and/or MAPKs to promote specialized cell division, functions in clathrin-mediated membrane transport and acts as potential substrate receptor of CUL4 E3 ubiquitin ligases. Quantitative RT-PCR and comparative RNAseq analyses of laser microdissected nucellar cells confirmed PN-SCD1 upregulation in florets of apomictic plants and revealed that overexpression takes place just before the onset of apospory initials. Moreover, we found that several SCD1 molecular partners are expressed in P. notatum florets and upregulated in apomictic plants. Our results disclosed a specific vesicle trafficking molecular pathway epigenetically modulated during apomixis.Fil: Bocchini, Marika. Università di Perugia; ItaliaFil: Galla, Giulio. Università di Padova; ItaliaFil: Pupilli, Fulvio. Consiglio Nazionale delle Ricerche; ItaliaFil: Bellucci, Michele. Consiglio Nazionale delle Ricerche; ItaliaFil: Barcaccia, Gianni. Università di Padova; ItaliaFil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Albertini, Emidio. Università di Perugia; ItaliaNature Publishing Group2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91961Bocchini, Marika; Galla, Giulio; Pupilli, Fulvio; Bellucci, Michele; Barcaccia, Gianni; et al.; The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-112045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-21220-4info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-21220-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:25Zoai:ri.conicet.gov.ar:11336/91961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:25.472CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes
title The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes
spellingShingle The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes
Bocchini, Marika
APOMIXIS
PASPALUM NOTATUM
METHYLATION
SCD1
title_short The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes
title_full The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes
title_fullStr The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes
title_full_unstemmed The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes
title_sort The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes
dc.creator.none.fl_str_mv Bocchini, Marika
Galla, Giulio
Pupilli, Fulvio
Bellucci, Michele
Barcaccia, Gianni
Ortiz, Juan Pablo Amelio
Pessino, Silvina Claudia
Albertini, Emidio
author Bocchini, Marika
author_facet Bocchini, Marika
Galla, Giulio
Pupilli, Fulvio
Bellucci, Michele
Barcaccia, Gianni
Ortiz, Juan Pablo Amelio
Pessino, Silvina Claudia
Albertini, Emidio
author_role author
author2 Galla, Giulio
Pupilli, Fulvio
Bellucci, Michele
Barcaccia, Gianni
Ortiz, Juan Pablo Amelio
Pessino, Silvina Claudia
Albertini, Emidio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOMIXIS
PASPALUM NOTATUM
METHYLATION
SCD1
topic APOMIXIS
PASPALUM NOTATUM
METHYLATION
SCD1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Apomixis (asexual reproduction through seeds) is considered a deviation of the sexual reproductive pathway leading to the development of clonal progenies genetically identical to the mother plant. Here we used the Methylation-Sensitive Amplification Polymorphism (MSAP) technique to characterize cytosine methylation patterns occurring in florets of sexual and aposporous Paspalum notatum genotypes, in order to identify epigenetically-controlled genes putatively involved in apomixis development. From twelve polymorphic MSAP-derived sequences, one (PN-6.6, later renamed PN-SCD1) was selected due to its relevant annotation and differential representation in apomictic and sexual floral transcriptome libraries. PN-SCD1 encodes the DENN domain/WD repeat-containing protein SCD1, which interacts with RAB GTPases- and/or MAPKs to promote specialized cell division, functions in clathrin-mediated membrane transport and acts as potential substrate receptor of CUL4 E3 ubiquitin ligases. Quantitative RT-PCR and comparative RNAseq analyses of laser microdissected nucellar cells confirmed PN-SCD1 upregulation in florets of apomictic plants and revealed that overexpression takes place just before the onset of apospory initials. Moreover, we found that several SCD1 molecular partners are expressed in P. notatum florets and upregulated in apomictic plants. Our results disclosed a specific vesicle trafficking molecular pathway epigenetically modulated during apomixis.
Fil: Bocchini, Marika. Università di Perugia; Italia
Fil: Galla, Giulio. Università di Padova; Italia
Fil: Pupilli, Fulvio. Consiglio Nazionale delle Ricerche; Italia
Fil: Bellucci, Michele. Consiglio Nazionale delle Ricerche; Italia
Fil: Barcaccia, Gianni. Università di Padova; Italia
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Albertini, Emidio. Università di Perugia; Italia
description Apomixis (asexual reproduction through seeds) is considered a deviation of the sexual reproductive pathway leading to the development of clonal progenies genetically identical to the mother plant. Here we used the Methylation-Sensitive Amplification Polymorphism (MSAP) technique to characterize cytosine methylation patterns occurring in florets of sexual and aposporous Paspalum notatum genotypes, in order to identify epigenetically-controlled genes putatively involved in apomixis development. From twelve polymorphic MSAP-derived sequences, one (PN-6.6, later renamed PN-SCD1) was selected due to its relevant annotation and differential representation in apomictic and sexual floral transcriptome libraries. PN-SCD1 encodes the DENN domain/WD repeat-containing protein SCD1, which interacts with RAB GTPases- and/or MAPKs to promote specialized cell division, functions in clathrin-mediated membrane transport and acts as potential substrate receptor of CUL4 E3 ubiquitin ligases. Quantitative RT-PCR and comparative RNAseq analyses of laser microdissected nucellar cells confirmed PN-SCD1 upregulation in florets of apomictic plants and revealed that overexpression takes place just before the onset of apospory initials. Moreover, we found that several SCD1 molecular partners are expressed in P. notatum florets and upregulated in apomictic plants. Our results disclosed a specific vesicle trafficking molecular pathway epigenetically modulated during apomixis.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91961
Bocchini, Marika; Galla, Giulio; Pupilli, Fulvio; Bellucci, Michele; Barcaccia, Gianni; et al.; The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-11
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91961
identifier_str_mv Bocchini, Marika; Galla, Giulio; Pupilli, Fulvio; Bellucci, Michele; Barcaccia, Gianni; et al.; The vesicle trafficking regulator PN-SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-11
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-21220-4
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-21220-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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