Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus)

• Autores: Baudou, Federico Gastón; Giorgi, Exequiel David; Diaz, María Eugenia; Fusco, Luciano Sebastián; Municoy, Sofía; Desimone, Martín Federico; Leiva, Laura Cristina Ana; De Marzi, Mauricio Cesar
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: documento de conferencia
• Materia: Crotalus durissus terrificus; Venoms; Antivenoms
• Institución: Universidad Nacional del Nordeste

Fil: Baudou, Federico Gastón. Universidad Nacional de Lujan. Departamento de Ciencias Básicas; Argentina.
Fil: Giorgi, Exequiel David. Universidad Nacional de Lujan. Departamento de Ciencias Básicas; Argentina.
Fil: Diaz, María Eugenia. Universidad Nacional de Lujan. Departamento de Ciencias Básicas; Argentina.
Fil: Fusco, Luciano Sebastián. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Municoy, Sofía. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina.
Fil: Municoy, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química y Metabolismo del Fármaco; Argentina.
Fil: Desimone, Martín Federico. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina.
Fil: Desimone, Martín Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química y Metabolismo del Fármaco; Argentina.
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: De Marzi, Mauricio Cesar. Universidad Nacional de Lujan. Departamento de Ciencias Básicas; Argentina.
Snake antivenoms (AV) production involves successive inoculations of venom (V) in increasing form together with the addition of adjuvants (ADJ) that improve the immune response of animals destined for immunizations. On the other hand, nanoparticles (NP) are being studied with multiple purposes due to their potential therapeutic and immunomodulatory use, and for their ability to transport antigens and induce a specific response against it. Therefore, it is of interest to associate V to NP in order to replacing conventional ADJ in AV production. In this work, nanovenoms (NV) were synthesized by adsorption of Crotalus durissus terrificus (C.d.t.) V proteins with silica NP (NPsi) of 400 nm size (positive/negative charge, +/-). The NPsi (-) were synthesized according to the Stöber method, while a fraction was modified with APTES (NPsi +), and its load and size was verified by potential Z and DLS (dynamic light scattering) respectively. Then, 10 mg of NPsi +/- were incubated with 1 mg / ml of whole V in PBS for 3 h under stirring to favor proteins adsorption to the NPs surface. NV were photographed under MET and also a FTIR (Fourier Transform Infrared) was performed. Crotoxin presence, the main toxic component of C.d.t. venom, was analyzed by hemolysis radial test both in NV and supernatant. NV proteins desorbed by heat treatment were analyzed by SDS-PAGE and immunoblotting. NV FTIR spectra showed intermediate values between those that exhibited C.d.t. V and the NPsi separately. SDS-PAGE and immunoblotting tests confirmed the presence of proteins in NPsi particles and hemolytic halos demonstrated that NPsi +/- were capable of binding crotoxin molecules on their surface. The results reveal the presence of V in both types of NPsi, preserving its activity and therefore its native structure, evidences that allow progress in an upcoming study such as the evaluation of immunogenic activity in experimental animals.