Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : comparative characterization and synergism between Asp49 and Lys49 variants

• Autores: Bustillo, Soledad; Fernández, Julián; Chaves Araya, Stephanie; Angulo, Yamileth; Leiva, Laura Cristina Ana; Lomonte, Bruno
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Toxicon, 2019, vol. 168, p. 113-121.
• Materia: Snake venom; Bothrops diporus; Phospholipase A2; Synergism; Myotoxicity; Cytotoxicity
• Institución: Universidad Nacional del Nordeste

Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Fernández, Julián. Universidad de Costa Rica. Facultad de Microbiología; Costa Rica.
Fil: Chaves Araya, Stephanie. Universidad de Costa Rica. Facultad de Microbiología; Costa Rica.
Fil: Angulo, Yamileth. Universidad de Costa Rica. Facultad de Microbiología; Costa Rica.
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Lomonte, Bruno. Universidad de Costa Rica. Facultad de Microbiología; Costa Rica.
Bothrops diporus, previously considered a subspecies of the B. neuwiedi complex, is a medically relevant viperid in Northeastern Argentina. The venom of this species causes local tissue damage characterized by myonecrosis, hemorrhage, blistering, and edema. In the present study, two basic phospholipases A2 (PLA2-I and PLA2-II) were isolated from this venom, and their pathological effects upon murine skeletal muscle and myogenic cells in culture were analyzed. Partial amino acid sequencing showed that PLA2-I and PLA2-II are Asp49 and Lys49 PLA2s, respectively. In agreement with this, PLA2-I showed PLA2 activity, whereas PLA2-II did not. Functional assays revealed differences in their myotoxicity, cytotoxicity, and anti-adhesion activity, and in the ability to inhibit cell migration, all of which were greater for the Lys49 variant. Native electrophoresis showed that PLA2-I was less basic than PLA2-II. The two proteins act synergistically to affect the integrity of C2C12 myogenic cells, providing a further example of the concerted action of coexisting snake venom components. PLA2-I and PLA2-II, together with additional basic PLA2s revealed by RP-HPLC, probably play an important role in myonecrosis after envenomation by B. diporus.