Analysis of variation for apomictic reproduction in diploid Paspalum rufum

Autores
Delgado, Luciana; Galdeano, Florencia; Sartor, María Esperanza; Quarín, Camilo León; Espinoza, Francisco; Ortiz, Juan Pablo Amelio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Delgado, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Delgado, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Galdeano, Florencia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Quarín, Camilo León. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Quarín, Camilo León. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Background and Aims: The diploid cytotype of Paspalum rufum (Poaceae) reproduces sexually and is self-sterile; however, recurrent autopolyploidization through 2n + n fertilization and the ability for reproduction via apomixis have been documented in one genotype of the species. The objectives of this work were to analyse the variation in the functionality of apomixis components in diploid genotypes of P. rufum and to identify individuals with contrasting reproductive behaviours. Methods: Samples of five individuals from each of three natural populations of P. rufum (designated R2, R5 and R6) were used. Seeds were obtained after open pollination, selfing, conspecific interploidy crosses and interspecific interploidy self-pollination induction. The reproductive behaviour of each plant was determined by using the flow cytometric seed screen (FCSS) method. Embryo sacs were cleared using a series of ethanol and methyl salicylate solutions and observed microscopically. Key Results: In open pollination, all genotypes formed seeds by sexual means and no evidence of apomeiotic reproduction was detected. However, in conspecific interploidy crosses and interspecific interploidy self-pollination induction, variations in the reproductive pathways were observed. While all plants from populations R2 and R6 formed seeds exclusively by sexual means, three genotypes from the R5 population developed seeds from both meiotic and aposporous embryo sacs, and one of them (R5#49) through the complete apomictic pathway (apospory + parthenogenesis + pseudogamy). Cytoembryological observations revealed the presence of both meiotic and aposporous embryo sacs in all the genotypes analysed, suggesting that parthenogenesis could be uncoupled from apospory in some genotypes. Conclusions: The results presented demonstrate the existence of variation in the functionality of apomixis components in natural diploid genotypes of P. rufum and have identified individuals with contrasting reproductive behaviours. Genotypes identified here can be crossed to generate segregating populations in order to study apomixis determinants at the diploid level. Moreover, analysis of their expression patterns, quantification of their transcript levels and an understanding of their regulation mechanisms could help to design new strategies for recreating apomixis in a diploid genome environment.
Fuente
Annals of Botany, 2014, vol. 113, no. 7, p. 1211–1218.
Materia
Apomixis
diploid genotype
Paspalum rufum
Poaceae
reproduction
seed development
plant mating systems
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/58284

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network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Analysis of variation for apomictic reproduction in diploid Paspalum rufumDelgado, LucianaGaldeano, FlorenciaSartor, María EsperanzaQuarín, Camilo LeónEspinoza, FranciscoOrtiz, Juan Pablo AmelioApomixisdiploid genotypePaspalum rufumPoaceaereproductionseed developmentplant mating systemsFil: Delgado, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Delgado, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Galdeano, Florencia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Quarín, Camilo León. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Quarín, Camilo León. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Background and Aims: The diploid cytotype of Paspalum rufum (Poaceae) reproduces sexually and is self-sterile; however, recurrent autopolyploidization through 2n + n fertilization and the ability for reproduction via apomixis have been documented in one genotype of the species. The objectives of this work were to analyse the variation in the functionality of apomixis components in diploid genotypes of P. rufum and to identify individuals with contrasting reproductive behaviours. Methods: Samples of five individuals from each of three natural populations of P. rufum (designated R2, R5 and R6) were used. Seeds were obtained after open pollination, selfing, conspecific interploidy crosses and interspecific interploidy self-pollination induction. The reproductive behaviour of each plant was determined by using the flow cytometric seed screen (FCSS) method. Embryo sacs were cleared using a series of ethanol and methyl salicylate solutions and observed microscopically. Key Results: In open pollination, all genotypes formed seeds by sexual means and no evidence of apomeiotic reproduction was detected. However, in conspecific interploidy crosses and interspecific interploidy self-pollination induction, variations in the reproductive pathways were observed. While all plants from populations R2 and R6 formed seeds exclusively by sexual means, three genotypes from the R5 population developed seeds from both meiotic and aposporous embryo sacs, and one of them (R5#49) through the complete apomictic pathway (apospory + parthenogenesis + pseudogamy). Cytoembryological observations revealed the presence of both meiotic and aposporous embryo sacs in all the genotypes analysed, suggesting that parthenogenesis could be uncoupled from apospory in some genotypes. Conclusions: The results presented demonstrate the existence of variation in the functionality of apomixis components in natural diploid genotypes of P. rufum and have identified individuals with contrasting reproductive behaviours. Genotypes identified here can be crossed to generate segregating populations in order to study apomixis determinants at the diploid level. Moreover, analysis of their expression patterns, quantification of their transcript levels and an understanding of their regulation mechanisms could help to design new strategies for recreating apomixis in a diploid genome environment.Oxford University Press2014-04-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 1211-1218application/pdfDelgado, Luciana, et al., 2014. Analysis of variation for apomictic reproduction in diploid Paspalum rufum. Annals of Botany. Oxford: Oxford University Press, vol. 113, no. 7, p. 1211-1218. E-ISSN 1095-8290. DOI https://doi.org/10.1093/aob/mcu0560305-7364http://repositorio.unne.edu.ar/handle/123456789/58284Annals of Botany, 2014, vol. 113, no. 7, p. 1211–1218.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.1093/aob/mcu056info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:26Zoai:repositorio.unne.edu.ar:123456789/58284instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:26.791Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Analysis of variation for apomictic reproduction in diploid Paspalum rufum
title Analysis of variation for apomictic reproduction in diploid Paspalum rufum
spellingShingle Analysis of variation for apomictic reproduction in diploid Paspalum rufum
Delgado, Luciana
Apomixis
diploid genotype
Paspalum rufum
Poaceae
reproduction
seed development
plant mating systems
title_short Analysis of variation for apomictic reproduction in diploid Paspalum rufum
title_full Analysis of variation for apomictic reproduction in diploid Paspalum rufum
title_fullStr Analysis of variation for apomictic reproduction in diploid Paspalum rufum
title_full_unstemmed Analysis of variation for apomictic reproduction in diploid Paspalum rufum
title_sort Analysis of variation for apomictic reproduction in diploid Paspalum rufum
dc.creator.none.fl_str_mv Delgado, Luciana
Galdeano, Florencia
Sartor, María Esperanza
Quarín, Camilo León
Espinoza, Francisco
Ortiz, Juan Pablo Amelio
author Delgado, Luciana
author_facet Delgado, Luciana
Galdeano, Florencia
Sartor, María Esperanza
Quarín, Camilo León
Espinoza, Francisco
Ortiz, Juan Pablo Amelio
author_role author
author2 Galdeano, Florencia
Sartor, María Esperanza
Quarín, Camilo León
Espinoza, Francisco
Ortiz, Juan Pablo Amelio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Apomixis
diploid genotype
Paspalum rufum
Poaceae
reproduction
seed development
plant mating systems
topic Apomixis
diploid genotype
Paspalum rufum
Poaceae
reproduction
seed development
plant mating systems
dc.description.none.fl_txt_mv Fil: Delgado, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Delgado, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Galdeano, Florencia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Quarín, Camilo León. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Quarín, Camilo León. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Background and Aims: The diploid cytotype of Paspalum rufum (Poaceae) reproduces sexually and is self-sterile; however, recurrent autopolyploidization through 2n + n fertilization and the ability for reproduction via apomixis have been documented in one genotype of the species. The objectives of this work were to analyse the variation in the functionality of apomixis components in diploid genotypes of P. rufum and to identify individuals with contrasting reproductive behaviours. Methods: Samples of five individuals from each of three natural populations of P. rufum (designated R2, R5 and R6) were used. Seeds were obtained after open pollination, selfing, conspecific interploidy crosses and interspecific interploidy self-pollination induction. The reproductive behaviour of each plant was determined by using the flow cytometric seed screen (FCSS) method. Embryo sacs were cleared using a series of ethanol and methyl salicylate solutions and observed microscopically. Key Results: In open pollination, all genotypes formed seeds by sexual means and no evidence of apomeiotic reproduction was detected. However, in conspecific interploidy crosses and interspecific interploidy self-pollination induction, variations in the reproductive pathways were observed. While all plants from populations R2 and R6 formed seeds exclusively by sexual means, three genotypes from the R5 population developed seeds from both meiotic and aposporous embryo sacs, and one of them (R5#49) through the complete apomictic pathway (apospory + parthenogenesis + pseudogamy). Cytoembryological observations revealed the presence of both meiotic and aposporous embryo sacs in all the genotypes analysed, suggesting that parthenogenesis could be uncoupled from apospory in some genotypes. Conclusions: The results presented demonstrate the existence of variation in the functionality of apomixis components in natural diploid genotypes of P. rufum and have identified individuals with contrasting reproductive behaviours. Genotypes identified here can be crossed to generate segregating populations in order to study apomixis determinants at the diploid level. Moreover, analysis of their expression patterns, quantification of their transcript levels and an understanding of their regulation mechanisms could help to design new strategies for recreating apomixis in a diploid genome environment.
description Fil: Delgado, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Delgado, Luciana, et al., 2014. Analysis of variation for apomictic reproduction in diploid Paspalum rufum. Annals of Botany. Oxford: Oxford University Press, vol. 113, no. 7, p. 1211-1218. E-ISSN 1095-8290. DOI https://doi.org/10.1093/aob/mcu056
0305-7364
http://repositorio.unne.edu.ar/handle/123456789/58284
identifier_str_mv Delgado, Luciana, et al., 2014. Analysis of variation for apomictic reproduction in diploid Paspalum rufum. Annals of Botany. Oxford: Oxford University Press, vol. 113, no. 7, p. 1211-1218. E-ISSN 1095-8290. DOI https://doi.org/10.1093/aob/mcu056
0305-7364
url http://repositorio.unne.edu.ar/handle/123456789/58284
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1093/aob/mcu056
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 1211-1218
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Annals of Botany, 2014, vol. 113, no. 7, p. 1211–1218.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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