Analysis of variation for apomictic reproduction in diploid Paspalum rufum
- Autores
- Delgado, Luciana; Galdeano, Florencia; Sartor, María Esperanza; Quarín, Camilo León; Espinoza, Francisco; Ortiz, Juan Pablo Amelio
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Delgado, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Delgado, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Galdeano, Florencia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Quarín, Camilo León. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Quarín, Camilo León. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Background and Aims: The diploid cytotype of Paspalum rufum (Poaceae) reproduces sexually and is self-sterile; however, recurrent autopolyploidization through 2n + n fertilization and the ability for reproduction via apomixis have been documented in one genotype of the species. The objectives of this work were to analyse the variation in the functionality of apomixis components in diploid genotypes of P. rufum and to identify individuals with contrasting reproductive behaviours. Methods: Samples of five individuals from each of three natural populations of P. rufum (designated R2, R5 and R6) were used. Seeds were obtained after open pollination, selfing, conspecific interploidy crosses and interspecific interploidy self-pollination induction. The reproductive behaviour of each plant was determined by using the flow cytometric seed screen (FCSS) method. Embryo sacs were cleared using a series of ethanol and methyl salicylate solutions and observed microscopically. Key Results: In open pollination, all genotypes formed seeds by sexual means and no evidence of apomeiotic reproduction was detected. However, in conspecific interploidy crosses and interspecific interploidy self-pollination induction, variations in the reproductive pathways were observed. While all plants from populations R2 and R6 formed seeds exclusively by sexual means, three genotypes from the R5 population developed seeds from both meiotic and aposporous embryo sacs, and one of them (R5#49) through the complete apomictic pathway (apospory + parthenogenesis + pseudogamy). Cytoembryological observations revealed the presence of both meiotic and aposporous embryo sacs in all the genotypes analysed, suggesting that parthenogenesis could be uncoupled from apospory in some genotypes. Conclusions: The results presented demonstrate the existence of variation in the functionality of apomixis components in natural diploid genotypes of P. rufum and have identified individuals with contrasting reproductive behaviours. Genotypes identified here can be crossed to generate segregating populations in order to study apomixis determinants at the diploid level. Moreover, analysis of their expression patterns, quantification of their transcript levels and an understanding of their regulation mechanisms could help to design new strategies for recreating apomixis in a diploid genome environment. - Fuente
- Annals of Botany, 2014, vol. 113, no. 7, p. 1211–1218.
- Materia
-
Apomixis
diploid genotype
Paspalum rufum
Poaceae
reproduction
seed development
plant mating systems - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Universidad Nacional del Nordeste
- OAI Identificador
- oai:repositorio.unne.edu.ar:123456789/58284
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Analysis of variation for apomictic reproduction in diploid Paspalum rufumDelgado, LucianaGaldeano, FlorenciaSartor, María EsperanzaQuarín, Camilo LeónEspinoza, FranciscoOrtiz, Juan Pablo AmelioApomixisdiploid genotypePaspalum rufumPoaceaereproductionseed developmentplant mating systemsFil: Delgado, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Delgado, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Galdeano, Florencia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Quarín, Camilo León. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Quarín, Camilo León. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Background and Aims: The diploid cytotype of Paspalum rufum (Poaceae) reproduces sexually and is self-sterile; however, recurrent autopolyploidization through 2n + n fertilization and the ability for reproduction via apomixis have been documented in one genotype of the species. The objectives of this work were to analyse the variation in the functionality of apomixis components in diploid genotypes of P. rufum and to identify individuals with contrasting reproductive behaviours. Methods: Samples of five individuals from each of three natural populations of P. rufum (designated R2, R5 and R6) were used. Seeds were obtained after open pollination, selfing, conspecific interploidy crosses and interspecific interploidy self-pollination induction. The reproductive behaviour of each plant was determined by using the flow cytometric seed screen (FCSS) method. Embryo sacs were cleared using a series of ethanol and methyl salicylate solutions and observed microscopically. Key Results: In open pollination, all genotypes formed seeds by sexual means and no evidence of apomeiotic reproduction was detected. However, in conspecific interploidy crosses and interspecific interploidy self-pollination induction, variations in the reproductive pathways were observed. While all plants from populations R2 and R6 formed seeds exclusively by sexual means, three genotypes from the R5 population developed seeds from both meiotic and aposporous embryo sacs, and one of them (R5#49) through the complete apomictic pathway (apospory + parthenogenesis + pseudogamy). Cytoembryological observations revealed the presence of both meiotic and aposporous embryo sacs in all the genotypes analysed, suggesting that parthenogenesis could be uncoupled from apospory in some genotypes. Conclusions: The results presented demonstrate the existence of variation in the functionality of apomixis components in natural diploid genotypes of P. rufum and have identified individuals with contrasting reproductive behaviours. Genotypes identified here can be crossed to generate segregating populations in order to study apomixis determinants at the diploid level. Moreover, analysis of their expression patterns, quantification of their transcript levels and an understanding of their regulation mechanisms could help to design new strategies for recreating apomixis in a diploid genome environment.Oxford University Press2014-04-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 1211-1218application/pdfDelgado, Luciana, et al., 2014. Analysis of variation for apomictic reproduction in diploid Paspalum rufum. Annals of Botany. Oxford: Oxford University Press, vol. 113, no. 7, p. 1211-1218. E-ISSN 1095-8290. DOI https://doi.org/10.1093/aob/mcu0560305-7364http://repositorio.unne.edu.ar/handle/123456789/58284Annals of Botany, 2014, vol. 113, no. 7, p. 1211–1218.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.1093/aob/mcu056info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:26Zoai:repositorio.unne.edu.ar:123456789/58284instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:26.791Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse |
dc.title.none.fl_str_mv |
Analysis of variation for apomictic reproduction in diploid Paspalum rufum |
title |
Analysis of variation for apomictic reproduction in diploid Paspalum rufum |
spellingShingle |
Analysis of variation for apomictic reproduction in diploid Paspalum rufum Delgado, Luciana Apomixis diploid genotype Paspalum rufum Poaceae reproduction seed development plant mating systems |
title_short |
Analysis of variation for apomictic reproduction in diploid Paspalum rufum |
title_full |
Analysis of variation for apomictic reproduction in diploid Paspalum rufum |
title_fullStr |
Analysis of variation for apomictic reproduction in diploid Paspalum rufum |
title_full_unstemmed |
Analysis of variation for apomictic reproduction in diploid Paspalum rufum |
title_sort |
Analysis of variation for apomictic reproduction in diploid Paspalum rufum |
dc.creator.none.fl_str_mv |
Delgado, Luciana Galdeano, Florencia Sartor, María Esperanza Quarín, Camilo León Espinoza, Francisco Ortiz, Juan Pablo Amelio |
author |
Delgado, Luciana |
author_facet |
Delgado, Luciana Galdeano, Florencia Sartor, María Esperanza Quarín, Camilo León Espinoza, Francisco Ortiz, Juan Pablo Amelio |
author_role |
author |
author2 |
Galdeano, Florencia Sartor, María Esperanza Quarín, Camilo León Espinoza, Francisco Ortiz, Juan Pablo Amelio |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Apomixis diploid genotype Paspalum rufum Poaceae reproduction seed development plant mating systems |
topic |
Apomixis diploid genotype Paspalum rufum Poaceae reproduction seed development plant mating systems |
dc.description.none.fl_txt_mv |
Fil: Delgado, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Delgado, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Galdeano, Florencia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Quarín, Camilo León. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Quarín, Camilo León. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Background and Aims: The diploid cytotype of Paspalum rufum (Poaceae) reproduces sexually and is self-sterile; however, recurrent autopolyploidization through 2n + n fertilization and the ability for reproduction via apomixis have been documented in one genotype of the species. The objectives of this work were to analyse the variation in the functionality of apomixis components in diploid genotypes of P. rufum and to identify individuals with contrasting reproductive behaviours. Methods: Samples of five individuals from each of three natural populations of P. rufum (designated R2, R5 and R6) were used. Seeds were obtained after open pollination, selfing, conspecific interploidy crosses and interspecific interploidy self-pollination induction. The reproductive behaviour of each plant was determined by using the flow cytometric seed screen (FCSS) method. Embryo sacs were cleared using a series of ethanol and methyl salicylate solutions and observed microscopically. Key Results: In open pollination, all genotypes formed seeds by sexual means and no evidence of apomeiotic reproduction was detected. However, in conspecific interploidy crosses and interspecific interploidy self-pollination induction, variations in the reproductive pathways were observed. While all plants from populations R2 and R6 formed seeds exclusively by sexual means, three genotypes from the R5 population developed seeds from both meiotic and aposporous embryo sacs, and one of them (R5#49) through the complete apomictic pathway (apospory + parthenogenesis + pseudogamy). Cytoembryological observations revealed the presence of both meiotic and aposporous embryo sacs in all the genotypes analysed, suggesting that parthenogenesis could be uncoupled from apospory in some genotypes. Conclusions: The results presented demonstrate the existence of variation in the functionality of apomixis components in natural diploid genotypes of P. rufum and have identified individuals with contrasting reproductive behaviours. Genotypes identified here can be crossed to generate segregating populations in order to study apomixis determinants at the diploid level. Moreover, analysis of their expression patterns, quantification of their transcript levels and an understanding of their regulation mechanisms could help to design new strategies for recreating apomixis in a diploid genome environment. |
description |
Fil: Delgado, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-16 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
Delgado, Luciana, et al., 2014. Analysis of variation for apomictic reproduction in diploid Paspalum rufum. Annals of Botany. Oxford: Oxford University Press, vol. 113, no. 7, p. 1211-1218. E-ISSN 1095-8290. DOI https://doi.org/10.1093/aob/mcu056 0305-7364 http://repositorio.unne.edu.ar/handle/123456789/58284 |
identifier_str_mv |
Delgado, Luciana, et al., 2014. Analysis of variation for apomictic reproduction in diploid Paspalum rufum. Annals of Botany. Oxford: Oxford University Press, vol. 113, no. 7, p. 1211-1218. E-ISSN 1095-8290. DOI https://doi.org/10.1093/aob/mcu056 0305-7364 |
url |
http://repositorio.unne.edu.ar/handle/123456789/58284 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://doi.org/10.1093/aob/mcu056 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina |
dc.format.none.fl_str_mv |
application/pdf p. 1211-1218 application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
Annals of Botany, 2014, vol. 113, no. 7, p. 1211–1218. reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) instname:Universidad Nacional del Nordeste |
reponame_str |
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
collection |
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
instname_str |
Universidad Nacional del Nordeste |
repository.name.fl_str_mv |
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste |
repository.mail.fl_str_mv |
ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar |
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1844621690008502272 |
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12.559606 |