Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells

Autores
Bustillo, Soledad; Gay, Claudia C.; García Denegri, María Emilia; Ponce-Soto, Luis Alberto; Bal de Kier Joffé, Elisa; Acosta, Ofelia Cristina; Leiva, Laura Cristina Ana
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.
Fil: Gay, Claudia Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.
Fil: García Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.
Fil: García Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Ponce-Soto, Luis Alberto. Universidade Estadual de Campinas. Instituto de Biología; Brasil.
Fil: Bal de Kier Joffé, Elisa. Instituto de Oncología Angel H. Roffo; Argentina.Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Leiva, Laura Cristina Ana. Instituto de Oncología Angel H. Roffo; Argentina.
Acute muscle damage, myonecrosis, is one of the main characteristics of envenoming by Bothrops genus. In this in vitro study we investigated the role of a metalloproteinase (baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited by Bothrops alternatus venom. Baltergin metalloproteinase purified from the venom exerted a toxic effect on C2C12 myoblast cells (CC50: 583.34 mg/mL) which involved morphological alterations compatible with apoptosis/anoikis. On the contrary, the most abundant PLA2 isolated from this venom did not exhibit cytotoxicity at times and doses tested. However, when myoblasts were treated with both enzymes together, synergic activity was demonstrated. Neutralization of the venom with specific antibodies (IgG anti-baltergin and IgG anti-PLA2) confirmed this synergism.
Fuente
Toxicon, 2012, vol. 59, p. 338–343.
Materia
Bothrops alternatus
Cytotoxicity
Phospholipase A2
Metalloproteinase
Baltergin
C2C12
Synergism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/59352
Acceder
id RIUNNE_8742ba383f83194867793e5d88540f3e
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/59352
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cellsBustillo, SoledadGay, Claudia C.García Denegri, María EmiliaPonce-Soto, Luis AlbertoBal de Kier Joffé, ElisaAcosta, Ofelia CristinaLeiva, Laura Cristina AnaBothrops alternatusCytotoxicityPhospholipase A2MetalloproteinaseBalterginC2C12SynergismFil: Bustillo, Soledad. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.Fil: Gay, Claudia Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.Fil: García Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.Fil: García Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Ponce-Soto, Luis Alberto. Universidade Estadual de Campinas. Instituto de Biología; Brasil.Fil: Bal de Kier Joffé, Elisa. Instituto de Oncología Angel H. Roffo; Argentina.Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Leiva, Laura Cristina Ana. Instituto de Oncología Angel H. Roffo; Argentina.Acute muscle damage, myonecrosis, is one of the main characteristics of envenoming by Bothrops genus. In this in vitro study we investigated the role of a metalloproteinase (baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited by Bothrops alternatus venom. Baltergin metalloproteinase purified from the venom exerted a toxic effect on C2C12 myoblast cells (CC50: 583.34 mg/mL) which involved morphological alterations compatible with apoptosis/anoikis. On the contrary, the most abundant PLA2 isolated from this venom did not exhibit cytotoxicity at times and doses tested. However, when myoblasts were treated with both enzymes together, synergic activity was demonstrated. Neutralization of the venom with specific antibodies (IgG anti-baltergin and IgG anti-PLA2) confirmed this synergism.Elsevier2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 338-343application/pdfBustillo, Soledad, et al., 2012. Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells. Toxicon. Oxford: Elsevier, vol. 59, p. 338–343. ISSN 1879-31500041-0101http://repositorio.unne.edu.ar/handle/123456789/59352Toxicon, 2012, vol. 59, p. 338–343.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.1016/j.toxicon.2011.11.007info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2026-01-08T11:18:57Zoai:repositorio.unne.edu.ar:123456789/59352instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-01-08 11:18:57.505Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
title Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
spellingShingle Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
Bustillo, Soledad
Bothrops alternatus
Cytotoxicity
Phospholipase A2
Metalloproteinase
Baltergin
C2C12
Synergism
title_short Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
title_full Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
title_fullStr Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
title_full_unstemmed Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
title_sort Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
dc.creator.none.fl_str_mv Bustillo, Soledad
Gay, Claudia C.
García Denegri, María Emilia
Ponce-Soto, Luis Alberto
Bal de Kier Joffé, Elisa
Acosta, Ofelia Cristina
Leiva, Laura Cristina Ana
author Bustillo, Soledad
author_facet Bustillo, Soledad
Gay, Claudia C.
García Denegri, María Emilia
Ponce-Soto, Luis Alberto
Bal de Kier Joffé, Elisa
Acosta, Ofelia Cristina
Leiva, Laura Cristina Ana
author_role author
author2 Gay, Claudia C.
García Denegri, María Emilia
Ponce-Soto, Luis Alberto
Bal de Kier Joffé, Elisa
Acosta, Ofelia Cristina
Leiva, Laura Cristina Ana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bothrops alternatus
Cytotoxicity
Phospholipase A2
Metalloproteinase
Baltergin
C2C12
Synergism
topic Bothrops alternatus
Cytotoxicity
Phospholipase A2
Metalloproteinase
Baltergin
C2C12
Synergism
dc.description.none.fl_txt_mv Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.
Fil: Gay, Claudia Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.
Fil: García Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.
Fil: García Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Ponce-Soto, Luis Alberto. Universidade Estadual de Campinas. Instituto de Biología; Brasil.
Fil: Bal de Kier Joffé, Elisa. Instituto de Oncología Angel H. Roffo; Argentina.Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Leiva, Laura Cristina Ana. Instituto de Oncología Angel H. Roffo; Argentina.
Acute muscle damage, myonecrosis, is one of the main characteristics of envenoming by Bothrops genus. In this in vitro study we investigated the role of a metalloproteinase (baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited by Bothrops alternatus venom. Baltergin metalloproteinase purified from the venom exerted a toxic effect on C2C12 myoblast cells (CC50: 583.34 mg/mL) which involved morphological alterations compatible with apoptosis/anoikis. On the contrary, the most abundant PLA2 isolated from this venom did not exhibit cytotoxicity at times and doses tested. However, when myoblasts were treated with both enzymes together, synergic activity was demonstrated. Neutralization of the venom with specific antibodies (IgG anti-baltergin and IgG anti-PLA2) confirmed this synergism.
description Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Bustillo, Soledad, et al., 2012. Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells. Toxicon. Oxford: Elsevier, vol. 59, p. 338–343. ISSN 1879-3150
0041-0101
http://repositorio.unne.edu.ar/handle/123456789/59352
identifier_str_mv Bustillo, Soledad, et al., 2012. Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells. Toxicon. Oxford: Elsevier, vol. 59, p. 338–343. ISSN 1879-3150
0041-0101
url http://repositorio.unne.edu.ar/handle/123456789/59352
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.toxicon.2011.11.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 338-343
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Toxicon, 2012, vol. 59, p. 338–343.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1853761434690781184
score 13.25844

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