In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
- Autores
- Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Ángeles; Ortiz Diez, Gustavo; Martin Espada, Carmen; Payá, María Jesús; Giusiano, Gustavo Emilio
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM; España.
Fil: Sosa, María de los Ángeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
Fil: Tur Tur, Cristina. SPDI, CAP Manso; España.
Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Ortiz Diez, Gustavo. Universidad Alfonso X El sabrio. Hospital clínico veterinario; España.
Fil: Martin Espada, Carmen. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Payá, María Jesús. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Labo- ratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which pre- sented the highest MICs and widest range of concentrations. Ketoconazole and itrac- onazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more- susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malas- sezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treat- ment regimen. - Fuente
- Mycoses, 2013, vol. 56, no. 1, p. 571-575.
- Materia
-
Malassezia
Susceptibility
Topical antifungals
Systemic antifungals - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Universidad Nacional del Nordeste
- OAI Identificador
- oai:repositorio.unne.edu.ar:123456789/52710
Ver los metadatos del registro completo
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In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia speciesCarrillo Muñoz, Alfonso JavierRojas, Florencia DinorahTur Tur, CristinaSosa, María de los ÁngelesOrtiz Diez, GustavoMartin Espada, CarmenPayá, María JesúsGiusiano, Gustavo EmilioMalasseziaSusceptibilityTopical antifungalsSystemic antifungalsFil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM; España.Fil: Sosa, María de los Ángeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.Fil: Tur Tur, Cristina. SPDI, CAP Manso; España.Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid. Facultad de Veterinaria; España.Fil: Ortiz Diez, Gustavo. Universidad Alfonso X El sabrio. Hospital clínico veterinario; España.Fil: Martin Espada, Carmen. Universidad Complutense de Madrid. Facultad de Veterinaria; España.Fil: Payá, María Jesús. Universidad Complutense de Madrid. Facultad de Veterinaria; España.Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Labo- ratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which pre- sented the highest MICs and widest range of concentrations. Ketoconazole and itrac- onazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more- susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malas- sezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treat- ment regimen.Wiley2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 571-575application/pdfCarrillo Muñoz, Alfonso Javier, et al., 2013. In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses. Hoboken: Wiley, vol. 56, no. 1, p. 571-575. ISSN 0933-7407.0933-7407http://repositorio.unne.edu.ar/handle/123456789/52710Mycoses, 2013, vol. 56, no. 1, p. 571-575.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:29:15Zoai:repositorio.unne.edu.ar:123456789/52710instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:29:16.007Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse |
dc.title.none.fl_str_mv |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
spellingShingle |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species Carrillo Muñoz, Alfonso Javier Malassezia Susceptibility Topical antifungals Systemic antifungals |
title_short |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title_full |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title_fullStr |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title_full_unstemmed |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title_sort |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
dc.creator.none.fl_str_mv |
Carrillo Muñoz, Alfonso Javier Rojas, Florencia Dinorah Tur Tur, Cristina Sosa, María de los Ángeles Ortiz Diez, Gustavo Martin Espada, Carmen Payá, María Jesús Giusiano, Gustavo Emilio |
author |
Carrillo Muñoz, Alfonso Javier |
author_facet |
Carrillo Muñoz, Alfonso Javier Rojas, Florencia Dinorah Tur Tur, Cristina Sosa, María de los Ángeles Ortiz Diez, Gustavo Martin Espada, Carmen Payá, María Jesús Giusiano, Gustavo Emilio |
author_role |
author |
author2 |
Rojas, Florencia Dinorah Tur Tur, Cristina Sosa, María de los Ángeles Ortiz Diez, Gustavo Martin Espada, Carmen Payá, María Jesús Giusiano, Gustavo Emilio |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Malassezia Susceptibility Topical antifungals Systemic antifungals |
topic |
Malassezia Susceptibility Topical antifungals Systemic antifungals |
dc.description.none.fl_txt_mv |
Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM; España. Fil: Sosa, María de los Ángeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Fil: Tur Tur, Cristina. SPDI, CAP Manso; España. Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid. Facultad de Veterinaria; España. Fil: Ortiz Diez, Gustavo. Universidad Alfonso X El sabrio. Hospital clínico veterinario; España. Fil: Martin Espada, Carmen. Universidad Complutense de Madrid. Facultad de Veterinaria; España. Fil: Payá, María Jesús. Universidad Complutense de Madrid. Facultad de Veterinaria; España. Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Labo- ratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which pre- sented the highest MICs and widest range of concentrations. Ketoconazole and itrac- onazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more- susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malas- sezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treat- ment regimen. |
description |
Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM; España. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
Carrillo Muñoz, Alfonso Javier, et al., 2013. In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses. Hoboken: Wiley, vol. 56, no. 1, p. 571-575. ISSN 0933-7407. 0933-7407 http://repositorio.unne.edu.ar/handle/123456789/52710 |
identifier_str_mv |
Carrillo Muñoz, Alfonso Javier, et al., 2013. In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses. Hoboken: Wiley, vol. 56, no. 1, p. 571-575. ISSN 0933-7407. 0933-7407 |
url |
http://repositorio.unne.edu.ar/handle/123456789/52710 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina |
dc.format.none.fl_str_mv |
application/pdf p. 571-575 application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
Mycoses, 2013, vol. 56, no. 1, p. 571-575. reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) instname:Universidad Nacional del Nordeste |
reponame_str |
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
collection |
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
instname_str |
Universidad Nacional del Nordeste |
repository.name.fl_str_mv |
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste |
repository.mail.fl_str_mv |
ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar |
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1844621659338702848 |
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12.559606 |