In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species

Autores
Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Ángeles; Ortiz Diez, Gustavo; Martin Espada, Carmen; Payá, María Jesús; Giusiano, Gustavo Emilio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM; España.
Fil: Sosa, María de los Ángeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
Fil: Tur Tur, Cristina. SPDI, CAP Manso; España.
Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Ortiz Diez, Gustavo. Universidad Alfonso X El sabrio. Hospital clínico veterinario; España.
Fil: Martin Espada, Carmen. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Payá, María Jesús. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Labo- ratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which pre- sented the highest MICs and widest range of concentrations. Ketoconazole and itrac- onazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more- susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malas- sezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treat- ment regimen.
Fuente
Mycoses, 2013, vol. 56, no. 1, p. 571-575.
Materia
Malassezia
Susceptibility
Topical antifungals
Systemic antifungals
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/52710

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network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia speciesCarrillo Muñoz, Alfonso JavierRojas, Florencia DinorahTur Tur, CristinaSosa, María de los ÁngelesOrtiz Diez, GustavoMartin Espada, CarmenPayá, María JesúsGiusiano, Gustavo EmilioMalasseziaSusceptibilityTopical antifungalsSystemic antifungalsFil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM; España.Fil: Sosa, María de los Ángeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.Fil: Tur Tur, Cristina. SPDI, CAP Manso; España.Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid. Facultad de Veterinaria; España.Fil: Ortiz Diez, Gustavo. Universidad Alfonso X El sabrio. Hospital clínico veterinario; España.Fil: Martin Espada, Carmen. Universidad Complutense de Madrid. Facultad de Veterinaria; España.Fil: Payá, María Jesús. Universidad Complutense de Madrid. Facultad de Veterinaria; España.Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Labo- ratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which pre- sented the highest MICs and widest range of concentrations. Ketoconazole and itrac- onazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more- susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malas- sezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treat- ment regimen.Wiley2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 571-575application/pdfCarrillo Muñoz, Alfonso Javier, et al., 2013. In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses. Hoboken: Wiley, vol. 56, no. 1, p. 571-575. ISSN 0933-7407.0933-7407http://repositorio.unne.edu.ar/handle/123456789/52710Mycoses, 2013, vol. 56, no. 1, p. 571-575.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:29:15Zoai:repositorio.unne.edu.ar:123456789/52710instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:29:16.007Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
spellingShingle In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
Carrillo Muñoz, Alfonso Javier
Malassezia
Susceptibility
Topical antifungals
Systemic antifungals
title_short In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_full In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_fullStr In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_full_unstemmed In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_sort In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
dc.creator.none.fl_str_mv Carrillo Muñoz, Alfonso Javier
Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Ángeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
author Carrillo Muñoz, Alfonso Javier
author_facet Carrillo Muñoz, Alfonso Javier
Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Ángeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
author_role author
author2 Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Ángeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malassezia
Susceptibility
Topical antifungals
Systemic antifungals
topic Malassezia
Susceptibility
Topical antifungals
Systemic antifungals
dc.description.none.fl_txt_mv Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM; España.
Fil: Sosa, María de los Ángeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
Fil: Tur Tur, Cristina. SPDI, CAP Manso; España.
Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Ortiz Diez, Gustavo. Universidad Alfonso X El sabrio. Hospital clínico veterinario; España.
Fil: Martin Espada, Carmen. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Payá, María Jesús. Universidad Complutense de Madrid. Facultad de Veterinaria; España.
Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina.
The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Labo- ratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which pre- sented the highest MICs and widest range of concentrations. Ketoconazole and itrac- onazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more- susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malas- sezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treat- ment regimen.
description Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM; España.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Carrillo Muñoz, Alfonso Javier, et al., 2013. In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses. Hoboken: Wiley, vol. 56, no. 1, p. 571-575. ISSN 0933-7407.
0933-7407
http://repositorio.unne.edu.ar/handle/123456789/52710
identifier_str_mv Carrillo Muñoz, Alfonso Javier, et al., 2013. In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses. Hoboken: Wiley, vol. 56, no. 1, p. 571-575. ISSN 0933-7407.
0933-7407
url http://repositorio.unne.edu.ar/handle/123456789/52710
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 571-575
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Mycoses, 2013, vol. 56, no. 1, p. 571-575.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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