In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
- Autores
- Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Angeles; Ortiz Diez, Gustavo; Martin Espada, Carmen; Payá, María Jesús; Giusiano, Gustavo Emilio
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be moresusceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.
Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM, Barcelona; España
Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tur Tur, Cristina. SPDI, CAP Manso, Barcelona; España
Fil: Sosa, María de los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina
Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid; España
Fil: Martin Espada, Carmen. Universidad Complutense de Madrid; España
Fil: Payá, María Jesús. Universidad Complutense de Madrid; España
Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Malassezia
Susceptibility
Topical Antifungals
Systemic Antifungals. - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/8941
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In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia speciesCarrillo Muñoz, Alfonso JavierRojas, Florencia DinorahTur Tur, CristinaSosa, María de los AngelesOrtiz Diez, GustavoMartin Espada, CarmenPayá, María JesúsGiusiano, Gustavo EmilioMalasseziaSusceptibilityTopical AntifungalsSystemic Antifungals.https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be moresusceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM, Barcelona; EspañaFil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tur Tur, Cristina. SPDI, CAP Manso, Barcelona; EspañaFil: Sosa, María de los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; ArgentinaFil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid; EspañaFil: Martin Espada, Carmen. Universidad Complutense de Madrid; EspañaFil: Payá, María Jesús. Universidad Complutense de Madrid; EspañaFil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8941Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Angeles; Ortiz Diez, Gustavo; et al.; In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species; Wiley; Mycoses; 56; 1; 4-2013; 571-5750933-7407enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/myc.12076/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/myc.12076info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:05Zoai:ri.conicet.gov.ar:11336/8941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:06.21CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
spellingShingle |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species Carrillo Muñoz, Alfonso Javier Malassezia Susceptibility Topical Antifungals Systemic Antifungals. |
title_short |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title_full |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title_fullStr |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title_full_unstemmed |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
title_sort |
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species |
dc.creator.none.fl_str_mv |
Carrillo Muñoz, Alfonso Javier Rojas, Florencia Dinorah Tur Tur, Cristina Sosa, María de los Angeles Ortiz Diez, Gustavo Martin Espada, Carmen Payá, María Jesús Giusiano, Gustavo Emilio |
author |
Carrillo Muñoz, Alfonso Javier |
author_facet |
Carrillo Muñoz, Alfonso Javier Rojas, Florencia Dinorah Tur Tur, Cristina Sosa, María de los Angeles Ortiz Diez, Gustavo Martin Espada, Carmen Payá, María Jesús Giusiano, Gustavo Emilio |
author_role |
author |
author2 |
Rojas, Florencia Dinorah Tur Tur, Cristina Sosa, María de los Angeles Ortiz Diez, Gustavo Martin Espada, Carmen Payá, María Jesús Giusiano, Gustavo Emilio |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Malassezia Susceptibility Topical Antifungals Systemic Antifungals. |
topic |
Malassezia Susceptibility Topical Antifungals Systemic Antifungals. |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be moresusceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen. Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM, Barcelona; España Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tur Tur, Cristina. SPDI, CAP Manso, Barcelona; España Fil: Sosa, María de los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid; España Fil: Martin Espada, Carmen. Universidad Complutense de Madrid; España Fil: Payá, María Jesús. Universidad Complutense de Madrid; España Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be moresusceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/8941 Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Angeles; Ortiz Diez, Gustavo; et al.; In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species; Wiley; Mycoses; 56; 1; 4-2013; 571-575 0933-7407 |
url |
http://hdl.handle.net/11336/8941 |
identifier_str_mv |
Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Angeles; Ortiz Diez, Gustavo; et al.; In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species; Wiley; Mycoses; 56; 1; 4-2013; 571-575 0933-7407 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/myc.12076/abstract info:eu-repo/semantics/altIdentifier/doi/10.1111/myc.12076 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846781255400554496 |
score |
13.238319 |