In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species

Autores
Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Angeles; Ortiz Diez, Gustavo; Martin Espada, Carmen; Payá, María Jesús; Giusiano, Gustavo Emilio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be moresusceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.
Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM, Barcelona; España
Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tur Tur, Cristina. SPDI, CAP Manso, Barcelona; España
Fil: Sosa, María de los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina
Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid; España
Fil: Martin Espada, Carmen. Universidad Complutense de Madrid; España
Fil: Payá, María Jesús. Universidad Complutense de Madrid; España
Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Malassezia
Susceptibility
Topical Antifungals
Systemic Antifungals.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8941
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8941
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia speciesCarrillo Muñoz, Alfonso JavierRojas, Florencia DinorahTur Tur, CristinaSosa, María de los AngelesOrtiz Diez, GustavoMartin Espada, CarmenPayá, María JesúsGiusiano, Gustavo EmilioMalasseziaSusceptibilityTopical AntifungalsSystemic Antifungals.https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be moresusceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM, Barcelona; EspañaFil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tur Tur, Cristina. SPDI, CAP Manso, Barcelona; EspañaFil: Sosa, María de los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; ArgentinaFil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid; EspañaFil: Martin Espada, Carmen. Universidad Complutense de Madrid; EspañaFil: Payá, María Jesús. Universidad Complutense de Madrid; EspañaFil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8941Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Angeles; Ortiz Diez, Gustavo; et al.; In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species; Wiley; Mycoses; 56; 1; 4-2013; 571-5750933-7407enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/myc.12076/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/myc.12076info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:30Zoai:ri.conicet.gov.ar:11336/8941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:30.43CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
spellingShingle In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
Carrillo Muñoz, Alfonso Javier
Malassezia
Susceptibility
Topical Antifungals
Systemic Antifungals.
title_short In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_full In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_fullStr In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_full_unstemmed In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_sort In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
dc.creator.none.fl_str_mv Carrillo Muñoz, Alfonso Javier
Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Angeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
author Carrillo Muñoz, Alfonso Javier
author_facet Carrillo Muñoz, Alfonso Javier
Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Angeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
author_role author
author2 Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Angeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malassezia
Susceptibility
Topical Antifungals
Systemic Antifungals.
topic Malassezia
Susceptibility
Topical Antifungals
Systemic Antifungals.
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be moresusceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.
Fil: Carrillo Muñoz, Alfonso Javier. Departamento de Microbiología-Micología, ACIAM, Barcelona; España
Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tur Tur, Cristina. SPDI, CAP Manso, Barcelona; España
Fil: Sosa, María de los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina
Fil: Ortiz Diez, Gustavo. Universidad Complutense de Madrid; España
Fil: Martin Espada, Carmen. Universidad Complutense de Madrid; España
Fil: Payá, María Jesús. Universidad Complutense de Madrid; España
Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be moresusceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8941
Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Angeles; Ortiz Diez, Gustavo; et al.; In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species; Wiley; Mycoses; 56; 1; 4-2013; 571-575
0933-7407
url http://hdl.handle.net/11336/8941
identifier_str_mv Carrillo Muñoz, Alfonso Javier; Rojas, Florencia Dinorah; Tur Tur, Cristina; Sosa, María de los Angeles; Ortiz Diez, Gustavo; et al.; In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species; Wiley; Mycoses; 56; 1; 4-2013; 571-575
0933-7407
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/myc.12076/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/myc.12076
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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