Chromosome numbers and DNA content in some species of mecardonia (gratiolae, plantaginaceae)

• Autores: Sosa, María de las Mercedes; Angulo, María Betiana; Greppi, Julián Alejandro; Bugallo, Verónica
• Año de publicación: 2016
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Comparative Cytogenetics, 2016, vol. 10, no. 4, p. 769-780.
• Materia: Gratiolae; Polyploidy; Chromosome number; DNA content; Flow cytometry
• Institución: Universidad Nacional del Nordeste

Fil: Greppi, Julián Alejandro. Instituto Nacional de Tecnología Agropecuaria. Instituto de Floricultura; Argentina.
Fil: Angulo, María Betiana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Angulo, María Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Sosa, Maria de las Mercedes. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Sosa, María de las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Bugallo, Verónica. Instituto Nacional de Tecnología Agropecuaria. Instituto de Floricultura; Argentina.
Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploid (2n = 6x = 66). The results include the first report of the chromosome numbers for M. flagellaris (Chamisso & Schlechtendal, 1827) (2n = 22), M. grandiflora (Bentham) Pennell, 1946 (2n = 22), M. kamogawae Greppi & Hagiwara, 2011 (2n = 66), and Mecardonia sp. (2n = 44). The three ploidy levels here reported suggest that polyploidy is common in Mecardonia and appear to be an important factor in the evolution of this genus. The 2C- and 1Cx-values were also estimated in all the species. The 2C-values ranged from 1.91 to 5.29 pg. The 1Cx-values ranged from 0.88 to 1.03 pg. The general tendency indicated a decrease in the 1Cx-value with increasing ploidy level. The significance of the results is discussed in relation to taxonomy of the genus.