Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves

Autores
Acevedo, Raúl Maximiliano; Avico, Edgardo Hernán; González, Sergio; Acácio Rodrígues, Salvador; Rivarola, Máximo; Paniego, Norma; Nunes Nesi, Adriano; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.
Fil: Acevedo, Raúl Maximiliano. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Acevedo, Raúl Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Avico, Edgardo Hernán. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Avico, Edgardo Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: González, Sergio. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.
Fil: Acácio Rodrígues, Salvador. Universidade Federal de Viçosa. Departamento de Biología Vegetal; Brazil.
Fil: Rivarola, Máximo. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.
Fil: Paniego, Norma. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Tecnológicas; Argentina.
Materia
Abiotic stress
De novo transcriptome
Drought stress
Cellular stress response
Rna-seq
Transcription factors
Yerba mate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/9142
Acceder
id RIUNNE_26a7c44d5bfa141b2602e25741e4e519
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/9142
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leavesAcevedo, Raúl MaximilianoAvico, Edgardo HernánGonzález, SergioAcácio Rodrígues, SalvadorRivarola, MáximoPaniego, NormaNunes Nesi, AdrianoRuiz, Oscar AdolfoSansberro, Pedro AlfonsoAbiotic stressDe novo transcriptomeDrought stressCellular stress responseRna-seqTranscription factorsYerba mateIlex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.Fil: Acevedo, Raúl Maximiliano. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Acevedo, Raúl Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Avico, Edgardo Hernán. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Avico, Edgardo Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: González, Sergio. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.Fil: Acácio Rodrígues, Salvador. Universidade Federal de Viçosa. Departamento de Biología Vegetal; Brazil.Fil: Rivarola, Máximo. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.Fil: Paniego, Norma. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Tecnológicas; Argentina.Springer Nature2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfAcevedo, Raúl Maximiliano, et al., 2019. Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves. Plant. Germany: Springer Science,no, 1, p. 1-18. ISSN 1435-8107.http://repositorio.unne.edu.ar/handle/123456789/9142enghttps://link.springer.com/article/10.1007%2Fs00425-019-03178-3https://doi.org/10.1007/s00425-019-03178-3info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentinareponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordeste2025-09-29T14:30:08Zoai:repositorio.unne.edu.ar:123456789/9142instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:09.194Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
spellingShingle Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
Acevedo, Raúl Maximiliano
Abiotic stress
De novo transcriptome
Drought stress
Cellular stress response
Rna-seq
Transcription factors
Yerba mate
title_short Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_full Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_fullStr Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_full_unstemmed Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_sort Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
dc.creator.none.fl_str_mv Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
González, Sergio
Acácio Rodrígues, Salvador
Rivarola, Máximo
Paniego, Norma
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author Acevedo, Raúl Maximiliano
author_facet Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
González, Sergio
Acácio Rodrígues, Salvador
Rivarola, Máximo
Paniego, Norma
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_role author
author2 Avico, Edgardo Hernán
González, Sergio
Acácio Rodrígues, Salvador
Rivarola, Máximo
Paniego, Norma
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Abiotic stress
De novo transcriptome
Drought stress
Cellular stress response
Rna-seq
Transcription factors
Yerba mate
topic Abiotic stress
De novo transcriptome
Drought stress
Cellular stress response
Rna-seq
Transcription factors
Yerba mate
dc.description.none.fl_txt_mv Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.
Fil: Acevedo, Raúl Maximiliano. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Acevedo, Raúl Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Avico, Edgardo Hernán. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Avico, Edgardo Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: González, Sergio. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.
Fil: Acácio Rodrígues, Salvador. Universidade Federal de Viçosa. Departamento de Biología Vegetal; Brazil.
Fil: Rivarola, Máximo. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.
Fil: Paniego, Norma. Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología; Argentina.
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Tecnológicas; Argentina.
description Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Acevedo, Raúl Maximiliano, et al., 2019. Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves. Plant. Germany: Springer Science,no, 1, p. 1-18. ISSN 1435-8107.
http://repositorio.unne.edu.ar/handle/123456789/9142
identifier_str_mv Acevedo, Raúl Maximiliano, et al., 2019. Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves. Plant. Germany: Springer Science,no, 1, p. 1-18. ISSN 1435-8107.
url http://repositorio.unne.edu.ar/handle/123456789/9142
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://link.springer.com/article/10.1007%2Fs00425-019-03178-3
https://doi.org/10.1007/s00425-019-03178-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.559606

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