Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves

Autores
Acevedo, Raúl Maximiliano; Avico, Edgardo H.; Gonzalez, Sergio Alberto; Rodrigues Salvador, Acácio; Rivarola, Maximo Lisandro; Paniego, Norma Beatriz; Nunes Nesi, Adriano; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.
Instituto de Biotecnología
Fil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste. Facultad de Ciencias Agrarias. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Avico, Edgardo H. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste. Facultad de Ciencias Agrarias. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodrigues Salvador, Acácio. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nunes Nesi, Adriano. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fuente
Planta 250 (2) : 445-462. (August 2019)
Materia
Abiotic Stress
Transcription
Plant Response
Drought Resistance
Plant Water Relations
Estrés Abiótico
Ilex Paraguariensis
Transcripción
Mate
Respuesta de la Planta
Resistencia a la Sequía
Relaciones Planta Agua
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6685
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6685
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leavesAcevedo, Raúl MaximilianoAvico, Edgardo H.Gonzalez, Sergio AlbertoRodrigues Salvador, AcácioRivarola, Maximo LisandroPaniego, Norma BeatrizNunes Nesi, AdrianoRuiz, Oscar AdolfoSansberro, Pedro AlfonsoAbiotic StressTranscriptionPlant ResponseDrought ResistancePlant Water RelationsEstrés AbióticoIlex ParaguariensisTranscripciónMateRespuesta de la PlantaResistencia a la SequíaRelaciones Planta AguaIlex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.Instituto de BiotecnologíaFil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste. Facultad de Ciencias Agrarias. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Avico, Edgardo H. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste. Facultad de Ciencias Agrarias. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodrigues Salvador, Acácio. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; BrasilFil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nunes Nesi, Adriano. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; BrasilFil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaSpringer2020-01-16T11:33:33Z2020-01-16T11:33:33Z2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6685https://link.springer.com/article/10.1007%2Fs00425-019-03178-30032-09351432-2048 (Online)https://doi.org/10.1007/s00425-019-03178-3Planta 250 (2) : 445-462. (August 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:52Zoai:localhost:20.500.12123/6685instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:52.417INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
spellingShingle Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
Acevedo, Raúl Maximiliano
Abiotic Stress
Transcription
Plant Response
Drought Resistance
Plant Water Relations
Estrés Abiótico
Ilex Paraguariensis
Transcripción
Mate
Respuesta de la Planta
Resistencia a la Sequía
Relaciones Planta Agua
title_short Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_full Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_fullStr Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_full_unstemmed Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_sort Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
dc.creator.none.fl_str_mv Acevedo, Raúl Maximiliano
Avico, Edgardo H.
Gonzalez, Sergio Alberto
Rodrigues Salvador, Acácio
Rivarola, Maximo Lisandro
Paniego, Norma Beatriz
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author Acevedo, Raúl Maximiliano
author_facet Acevedo, Raúl Maximiliano
Avico, Edgardo H.
Gonzalez, Sergio Alberto
Rodrigues Salvador, Acácio
Rivarola, Maximo Lisandro
Paniego, Norma Beatriz
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_role author
author2 Avico, Edgardo H.
Gonzalez, Sergio Alberto
Rodrigues Salvador, Acácio
Rivarola, Maximo Lisandro
Paniego, Norma Beatriz
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Abiotic Stress
Transcription
Plant Response
Drought Resistance
Plant Water Relations
Estrés Abiótico
Ilex Paraguariensis
Transcripción
Mate
Respuesta de la Planta
Resistencia a la Sequía
Relaciones Planta Agua
topic Abiotic Stress
Transcription
Plant Response
Drought Resistance
Plant Water Relations
Estrés Abiótico
Ilex Paraguariensis
Transcripción
Mate
Respuesta de la Planta
Resistencia a la Sequía
Relaciones Planta Agua
dc.description.none.fl_txt_mv Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.
Instituto de Biotecnología
Fil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste. Facultad de Ciencias Agrarias. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Avico, Edgardo H. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste. Facultad de Ciencias Agrarias. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodrigues Salvador, Acácio. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nunes Nesi, Adriano. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
2020-01-16T11:33:33Z
2020-01-16T11:33:33Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6685
https://link.springer.com/article/10.1007%2Fs00425-019-03178-3
0032-0935
1432-2048 (Online)
https://doi.org/10.1007/s00425-019-03178-3
url http://hdl.handle.net/20.500.12123/6685
https://link.springer.com/article/10.1007%2Fs00425-019-03178-3
https://doi.org/10.1007/s00425-019-03178-3
identifier_str_mv 0032-0935
1432-2048 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Planta 250 (2) : 445-462. (August 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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