In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)

• Autores: Scocchi, Adriana M.; Mroginski, Luis Amado
• Año de publicación: 2004
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Phyton International Journal of Experimental Botany, 2004, vol. 73, p. 137-143.
• Materia: Conservation; Germplasm; In vitro culture; Melia azedarach; Meristem
• Institución: Universidad Nacional del Nordeste

Fil: Scocchi, Adriana M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Studies on in vitro storage of Melia azedarach –“paradise tree”- under slow-growth conditions were carried out to develop an efficient protocol for the con- servation of the genetic diversity of the crop. The response to different growth re- tardation treatments was examined using clone E. In vitro cultures of apical mer- istem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4oC on 1⁄4MS basal medium supplemented with 0.5μM BA. Shoot proliferation was observed on 67% of the recovered apical meristem-tips, after 12 months at 4oC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 μM IBA, during 4 days and subsequently transferred to MS medium lacking growth- regulators for 30 days. Complete plants were successfully transferred to the green- house.