In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)

Autores
Scocchi, A. M.; Mroginski, Luis Amado
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Studies on in vitro storage of Melia azedarach -"paradise tree"- under slow-growth conditions were carried out to develop an efficient protocol for the conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using clone E. In vitro cultures of apical meristem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4ºC on ¼ MS basal medium supplemented with 0.5 µM BA. Shoot proliferation was observed on 67 % of the recovered apical meristem-tips, after 12 months at 4ºC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 µM IBA, during 4 days and subsequently transferred to MS medium lacking growth-regulators for 30 days. Complete plants were successfully transferred to the greenhouse.
Fil: Scocchi, A. M.. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Materia
conservation
germplasm
in vitro culture
Melia azedarach
meristem
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45409
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/45409
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)Scocchi, A. M.Mroginski, Luis Amadoconservationgermplasmin vitro cultureMelia azedarachmeristemhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Studies on in vitro storage of Melia azedarach -"paradise tree"- under slow-growth conditions were carried out to develop an efficient protocol for the conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using clone E. In vitro cultures of apical meristem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4ºC on ¼ MS basal medium supplemented with 0.5 µM BA. Shoot proliferation was observed on 67 % of the recovered apical meristem-tips, after 12 months at 4ºC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 µM IBA, during 4 days and subsequently transferred to MS medium lacking growth-regulators for 30 days. Complete plants were successfully transferred to the greenhouse.Fil: Scocchi, A. M.. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFundación Romulo Raggio2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45409Scocchi, A. M.; Mroginski, Luis Amado; In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures); Fundación Romulo Raggio; Phyton - International Journal of Experimental Botany; 73; 12-2004; 137-1430031-94571851-5657CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/26fyxhinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:42:43Zoai:ri.conicet.gov.ar:11336/45409instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:42:43.592CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)
title In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)
spellingShingle In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)
Scocchi, A. M.
conservation
germplasm
in vitro culture
Melia azedarach
meristem
title_short In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)
title_full In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)
title_fullStr In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)
title_full_unstemmed In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)
title_sort In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)
dc.creator.none.fl_str_mv Scocchi, A. M.
Mroginski, Luis Amado
author Scocchi, A. M.
author_facet Scocchi, A. M.
Mroginski, Luis Amado
author_role author
author2 Mroginski, Luis Amado
author2_role author
dc.subject.none.fl_str_mv conservation
germplasm
in vitro culture
Melia azedarach
meristem
topic conservation
germplasm
in vitro culture
Melia azedarach
meristem
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Studies on in vitro storage of Melia azedarach -"paradise tree"- under slow-growth conditions were carried out to develop an efficient protocol for the conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using clone E. In vitro cultures of apical meristem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4ºC on ¼ MS basal medium supplemented with 0.5 µM BA. Shoot proliferation was observed on 67 % of the recovered apical meristem-tips, after 12 months at 4ºC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 µM IBA, during 4 days and subsequently transferred to MS medium lacking growth-regulators for 30 days. Complete plants were successfully transferred to the greenhouse.
Fil: Scocchi, A. M.. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description Studies on in vitro storage of Melia azedarach -"paradise tree"- under slow-growth conditions were carried out to develop an efficient protocol for the conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using clone E. In vitro cultures of apical meristem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4ºC on ¼ MS basal medium supplemented with 0.5 µM BA. Shoot proliferation was observed on 67 % of the recovered apical meristem-tips, after 12 months at 4ºC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 µM IBA, during 4 days and subsequently transferred to MS medium lacking growth-regulators for 30 days. Complete plants were successfully transferred to the greenhouse.
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45409
Scocchi, A. M.; Mroginski, Luis Amado; In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures); Fundación Romulo Raggio; Phyton - International Journal of Experimental Botany; 73; 12-2004; 137-143
0031-9457
1851-5657
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45409
identifier_str_mv Scocchi, A. M.; Mroginski, Luis Amado; In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures); Fundación Romulo Raggio; Phyton - International Journal of Experimental Botany; 73; 12-2004; 137-143
0031-9457
1851-5657
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/26fyxh
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundación Romulo Raggio
publisher.none.fl_str_mv Fundación Romulo Raggio
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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