Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples

Autores
Monzón, Celina María; Teglia, Carla M.; Delfino, Mario Raúl; Goicoechea, Héctor C.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Monzón, Celina M. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.
Fil: Monzón, Celina M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.
Fil: Teglia, Carla M. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.
Fil: Teglia, Carla M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.
Fil: Delfino, Mario Raúl. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.
Fil: Goicoechea, Héctor C. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.
Fil: Goicoechea, Héctor C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.
A dispersive liquid–liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L−1 for the three analytes).
Fuente
Microchemical Journal, 2016, vol. 127, p. 113-119.
Materia
DLLME
Glibenclamide
Gliclazide
Glimepiride
Chemometrics
HPLC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/27940
Acceder
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network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samplesMonzón, Celina MaríaTeglia, Carla M.Delfino, Mario RaúlGoicoechea, Héctor C.DLLMEGlibenclamideGliclazideGlimepirideChemometricsHPLCFil: Monzón, Celina M. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.Fil: Monzón, Celina M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.Fil: Teglia, Carla M. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.Fil: Teglia, Carla M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.Fil: Delfino, Mario Raúl. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.Fil: Goicoechea, Héctor C. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.Fil: Goicoechea, Héctor C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.A dispersive liquid–liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L−1 for the three analytes).Elsevier2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfMonzón, Celina M., et al., 2016. Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples. Microchemical Journal. Ámsterdam: Elsevier, vol. 127, p. 113-119. ISSN 0026-265X.0026-265Xhttp://repositorio.unne.edu.ar/handle/123456789/27940Microchemical Journal, 2016, vol. 127, p. 113-119.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttp://dx.doi.org/10.1016/j.microc.2016.02.011info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:20Zoai:repositorio.unne.edu.ar:123456789/27940instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:20.413Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
spellingShingle Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
Monzón, Celina María
DLLME
Glibenclamide
Gliclazide
Glimepiride
Chemometrics
HPLC
title_short Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_full Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_fullStr Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_full_unstemmed Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_sort Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
dc.creator.none.fl_str_mv Monzón, Celina María
Teglia, Carla M.
Delfino, Mario Raúl
Goicoechea, Héctor C.
author Monzón, Celina María
author_facet Monzón, Celina María
Teglia, Carla M.
Delfino, Mario Raúl
Goicoechea, Héctor C.
author_role author
author2 Teglia, Carla M.
Delfino, Mario Raúl
Goicoechea, Héctor C.
author2_role author
author
author
dc.subject.none.fl_str_mv DLLME
Glibenclamide
Gliclazide
Glimepiride
Chemometrics
HPLC
topic DLLME
Glibenclamide
Gliclazide
Glimepiride
Chemometrics
HPLC
dc.description.none.fl_txt_mv Fil: Monzón, Celina M. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.
Fil: Monzón, Celina M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.
Fil: Teglia, Carla M. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.
Fil: Teglia, Carla M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.
Fil: Delfino, Mario Raúl. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.
Fil: Goicoechea, Héctor C. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.
Fil: Goicoechea, Héctor C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico; Argentina.
A dispersive liquid–liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L−1 for the three analytes).
description Fil: Monzón, Celina M. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Monzón, Celina M., et al., 2016. Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples. Microchemical Journal. Ámsterdam: Elsevier, vol. 127, p. 113-119. ISSN 0026-265X.
0026-265X
http://repositorio.unne.edu.ar/handle/123456789/27940
identifier_str_mv Monzón, Celina M., et al., 2016. Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples. Microchemical Journal. Ámsterdam: Elsevier, vol. 127, p. 113-119. ISSN 0026-265X.
0026-265X
url http://repositorio.unne.edu.ar/handle/123456789/27940
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.microc.2016.02.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Microchemical Journal, 2016, vol. 127, p. 113-119.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1844621687589437440
score 12.559606

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