Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples

Autores
Monzón, Celina María; Teglia, Carla Mariela; Delfino, Mario Raul; Goicoechea, Hector Casimiro
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A dispersive liquid-liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L-1 for the three analytes).
Fil: Monzón, Celina María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Teglia, Carla Mariela. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delfino, Mario Raul. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Chemometrics
Dllme
Glibenclamide
Gliclazide
Glimepiride
Hplc
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37780
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37780
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samplesMonzón, Celina MaríaTeglia, Carla MarielaDelfino, Mario RaulGoicoechea, Hector CasimiroChemometricsDllmeGlibenclamideGliclazideGlimepirideHplchttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A dispersive liquid-liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L-1 for the three analytes).Fil: Monzón, Celina María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; ArgentinaFil: Teglia, Carla Mariela. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Delfino, Mario Raul. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37780Monzón, Celina María; Teglia, Carla Mariela; Delfino, Mario Raul; Goicoechea, Hector Casimiro; Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples; Elsevier Science; Microchemical Journal; 127; 7-2016; 113-1190026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.02.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X1600032info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:25Zoai:ri.conicet.gov.ar:11336/37780instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:25.713CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
spellingShingle Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
Monzón, Celina María
Chemometrics
Dllme
Glibenclamide
Gliclazide
Glimepiride
Hplc
title_short Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_full Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_fullStr Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_full_unstemmed Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_sort Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
dc.creator.none.fl_str_mv Monzón, Celina María
Teglia, Carla Mariela
Delfino, Mario Raul
Goicoechea, Hector Casimiro
author Monzón, Celina María
author_facet Monzón, Celina María
Teglia, Carla Mariela
Delfino, Mario Raul
Goicoechea, Hector Casimiro
author_role author
author2 Teglia, Carla Mariela
Delfino, Mario Raul
Goicoechea, Hector Casimiro
author2_role author
author
author
dc.subject.none.fl_str_mv Chemometrics
Dllme
Glibenclamide
Gliclazide
Glimepiride
Hplc
topic Chemometrics
Dllme
Glibenclamide
Gliclazide
Glimepiride
Hplc
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A dispersive liquid-liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L-1 for the three analytes).
Fil: Monzón, Celina María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Teglia, Carla Mariela. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delfino, Mario Raul. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A dispersive liquid-liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L-1 for the three analytes).
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37780
Monzón, Celina María; Teglia, Carla Mariela; Delfino, Mario Raul; Goicoechea, Hector Casimiro; Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples; Elsevier Science; Microchemical Journal; 127; 7-2016; 113-119
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37780
identifier_str_mv Monzón, Celina María; Teglia, Carla Mariela; Delfino, Mario Raul; Goicoechea, Hector Casimiro; Chemometric optimization and validation of a novel dispersive liquid-liquid microextraction-HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples; Elsevier Science; Microchemical Journal; 127; 7-2016; 113-119
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.02.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X1600032
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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