Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity

Autores
Paulazo, Maria Alejandra; Klecha, Alicia Juana; Sterle, Helena Andrea; Valli, Eduardo; Torti, Horacio; Cayrol, María Florencia; Barreiro Arcos, María Laura; Cremaschi, Graciela A.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Paulazo, Maria Alejandra. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klecha, Alicia Juana. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Klecha, Alicia Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klecha, Alicia Juana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; Argentina
Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torti, Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; Argentina
Fil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cremaschi, Graciela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; Argentina
Abstract: Purpose Hypothyroidism has been shown to induce immunosuppression and both the thyroid status and immunity are affected by zinc deficiency. However, the impact of hypothyroidism on zinc metabolism and its possible relationship with the immune status has not yet been deeply explored. Here, our aim was to study whether hypothyroidism may alter zinc metabolism and thus lead to the impairment of T lymphocyte activity. Methods Variations in the distribution of zinc in the body were evaluated in PTU-treated hypothyroid mice. The effects of hypothyroidism and zinc deficiency were studied on T lymphocyte proliferation after stimulation both in vitro and in vivo. For in vitro assays, thyroid hormone-free or zinc chelator (TPEN or DTPA)-supplemented media were used. For in vivo assays, lymphocyte activity was evaluated in cells from hypothyroid, T3-treated, and zinc-supplemented mice. Results Hypothyroid mice showed lower levels of zinc in femur and lymph nodes than controls. T3 and zinc supplementation reversed these effects. In vitro, both thyroid hormone and zinc deficiency led to a decreased response to mitogen stimulation. However, only zinc deficiency was able to induce lymphocyte apoptosis. Mitogen-stimulated T cells from hypothyroid mice showed impaired proliferation, accompanied by decreased activation of PKC and lower levels of p-ERK, effects that were reversed by T3 replacement or zinc supplementation. Conclusions Our results show an important role of zinc deficiency in hypothyroid-mediated T-cell suppression and suggest the importance of evaluating zinc levels and restoring them when necessary to maintain an efficient immune response in hypothyroid patients.
Fuente
Endocrine. 2019, 66
Materia
GLANDULA TIROIDES
HIPOTIROIDISMO
LINFOCITOS T
ZINC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/14235
Acceder
id RIUCA_cd496653850537cbc89d8c0ab1182ad4
oai_identifier_str oai:ucacris:123456789/14235
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activityPaulazo, Maria AlejandraKlecha, Alicia JuanaSterle, Helena AndreaValli, EduardoTorti, HoracioCayrol, María FlorenciaBarreiro Arcos, María LauraCremaschi, Graciela A.GLANDULA TIROIDESHIPOTIROIDISMOLINFOCITOS TZINCFil: Paulazo, Maria Alejandra. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Klecha, Alicia Juana. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Klecha, Alicia Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Klecha, Alicia Juana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; ArgentinaFil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Torti, Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; ArgentinaFil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cremaschi, Graciela Alicia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cremaschi, Graciela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; ArgentinaAbstract: Purpose Hypothyroidism has been shown to induce immunosuppression and both the thyroid status and immunity are affected by zinc deficiency. However, the impact of hypothyroidism on zinc metabolism and its possible relationship with the immune status has not yet been deeply explored. Here, our aim was to study whether hypothyroidism may alter zinc metabolism and thus lead to the impairment of T lymphocyte activity. Methods Variations in the distribution of zinc in the body were evaluated in PTU-treated hypothyroid mice. The effects of hypothyroidism and zinc deficiency were studied on T lymphocyte proliferation after stimulation both in vitro and in vivo. For in vitro assays, thyroid hormone-free or zinc chelator (TPEN or DTPA)-supplemented media were used. For in vivo assays, lymphocyte activity was evaluated in cells from hypothyroid, T3-treated, and zinc-supplemented mice. Results Hypothyroid mice showed lower levels of zinc in femur and lymph nodes than controls. T3 and zinc supplementation reversed these effects. In vitro, both thyroid hormone and zinc deficiency led to a decreased response to mitogen stimulation. However, only zinc deficiency was able to induce lymphocyte apoptosis. Mitogen-stimulated T cells from hypothyroid mice showed impaired proliferation, accompanied by decreased activation of PKC and lower levels of p-ERK, effects that were reversed by T3 replacement or zinc supplementation. Conclusions Our results show an important role of zinc deficiency in hypothyroid-mediated T-cell suppression and suggest the importance of evaluating zinc levels and restoring them when necessary to maintain an efficient immune response in hypothyroid patients.Springer2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/142351559-010010.1007/s12020-019-01936-731056723Paulazo, M.A., et al. Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity [en línea]. Endocrine. 2019, 66 doi:10.1007/s12020-019-01936-7 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14235Endocrine. 2019, 66reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:58:38Zoai:ucacris:123456789/14235instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:58:38.36Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity
title Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity
spellingShingle Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity
Paulazo, Maria Alejandra
GLANDULA TIROIDES
HIPOTIROIDISMO
LINFOCITOS T
ZINC
title_short Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity
title_full Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity
title_fullStr Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity
title_full_unstemmed Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity
title_sort Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity
dc.creator.none.fl_str_mv Paulazo, Maria Alejandra
Klecha, Alicia Juana
Sterle, Helena Andrea
Valli, Eduardo
Torti, Horacio
Cayrol, María Florencia
Barreiro Arcos, María Laura
Cremaschi, Graciela A.
author Paulazo, Maria Alejandra
author_facet Paulazo, Maria Alejandra
Klecha, Alicia Juana
Sterle, Helena Andrea
Valli, Eduardo
Torti, Horacio
Cayrol, María Florencia
Barreiro Arcos, María Laura
Cremaschi, Graciela A.
author_role author
author2 Klecha, Alicia Juana
Sterle, Helena Andrea
Valli, Eduardo
Torti, Horacio
Cayrol, María Florencia
Barreiro Arcos, María Laura
Cremaschi, Graciela A.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GLANDULA TIROIDES
HIPOTIROIDISMO
LINFOCITOS T
ZINC
topic GLANDULA TIROIDES
HIPOTIROIDISMO
LINFOCITOS T
ZINC
dc.description.none.fl_txt_mv Fil: Paulazo, Maria Alejandra. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klecha, Alicia Juana. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Klecha, Alicia Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klecha, Alicia Juana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; Argentina
Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torti, Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; Argentina
Fil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cremaschi, Graciela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Física; Argentina
Abstract: Purpose Hypothyroidism has been shown to induce immunosuppression and both the thyroid status and immunity are affected by zinc deficiency. However, the impact of hypothyroidism on zinc metabolism and its possible relationship with the immune status has not yet been deeply explored. Here, our aim was to study whether hypothyroidism may alter zinc metabolism and thus lead to the impairment of T lymphocyte activity. Methods Variations in the distribution of zinc in the body were evaluated in PTU-treated hypothyroid mice. The effects of hypothyroidism and zinc deficiency were studied on T lymphocyte proliferation after stimulation both in vitro and in vivo. For in vitro assays, thyroid hormone-free or zinc chelator (TPEN or DTPA)-supplemented media were used. For in vivo assays, lymphocyte activity was evaluated in cells from hypothyroid, T3-treated, and zinc-supplemented mice. Results Hypothyroid mice showed lower levels of zinc in femur and lymph nodes than controls. T3 and zinc supplementation reversed these effects. In vitro, both thyroid hormone and zinc deficiency led to a decreased response to mitogen stimulation. However, only zinc deficiency was able to induce lymphocyte apoptosis. Mitogen-stimulated T cells from hypothyroid mice showed impaired proliferation, accompanied by decreased activation of PKC and lower levels of p-ERK, effects that were reversed by T3 replacement or zinc supplementation. Conclusions Our results show an important role of zinc deficiency in hypothyroid-mediated T-cell suppression and suggest the importance of evaluating zinc levels and restoring them when necessary to maintain an efficient immune response in hypothyroid patients.
description Fil: Paulazo, Maria Alejandra. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/14235
1559-0100
10.1007/s12020-019-01936-7
31056723
Paulazo, M.A., et al. Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity [en línea]. Endocrine. 2019, 66 doi:10.1007/s12020-019-01936-7 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14235
url https://repositorio.uca.edu.ar/handle/123456789/14235
identifier_str_mv 1559-0100
10.1007/s12020-019-01936-7
31056723
Paulazo, M.A., et al. Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity [en línea]. Endocrine. 2019, 66 doi:10.1007/s12020-019-01936-7 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14235
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Endocrine. 2019, 66
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.13397

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