First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain

Autores
Perelló, Analía E.; Olmo, Diego; Busquets, Antoni; Romero Munar, Antonia; Quetglas, Bàrbara Maria; Gost, Pere Antoni; Berbegal, M.; Armengol, J.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Fil: Perelló, Analía E. Pontificia Universidad Católica Argentina, Facultad de Ingeniería y Ciencias Agrarias. Laboratorio e producción vegetal; Argentina
Fil: Olmo, Diego. Serveis de Millora Agrària i Pesqueral; España
Fil: Busquets, Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Busquets, Antoni. Universitat de les Illes Balears. Consejo Superior de Investigaciones Científicas. Instituto Mediterráneo de Estudios Avanzados; España
Fil: Romero Munar, Antonia. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Department of Engineering; España
Fil: Gost, Pere Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Gost, Pere Antoni. Universitat de les Illes Balears. Department of Engineering; España
Fil: Berbegal, M. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; España
Fil: Armengol, J. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; España
Abstract: In 2021, grapevines (Vitis vinifera L.) cv. Callet growing in a commercial vineyard located at Pollença (northeast of the island of Majorca, Spain) showed severe symptoms of shoot blight during spring and early summer, with an incidence of 70%. Symptoms consisted of elongated cankered-like lesions, surrounded by water-soaked darker tissues, that developed at the base or around the middle nodes of the shoot. For fungal isolation, shoot samples with lesions were collected, surface disinfected with 2% NaCl for 90s, rinsed twice with deionized water and placed in Petri plates containing potato dextrose agar (PDA). The plates were incubated at 25°C under 12 h light-darkness for 6 days. Isolations consistently yielded on kind of fungal colonies that produced white mycelium and black spherical to elongated sclerotia (2 to 10 mm in diameter). Morphological characterization was consistent with the description of Sclerotinia sclerotiorum (Lib.) de Bary (Bolton et al. 2006). Three isolates (UIB 118-1, UIB 118-26, and UIB 129-41) were preserved and deposited in the Culture Collection of Microbiology-Faculty of Sciences, University of Balearic Islands, Spain. Genomic DNA was extracted from isolates UIB 118-26 and UIB 129-41 using the EZNA Miniprep Kit (Omega Bio-Tek, Norcross, GA). The internal transcribed spacer (ITS) region of ribosomal DNA, β-tubulin (BTUB) and calmodulin (CAL) gene regions were amplified using ITS1F-ITS4 (Gardes and Bruns, 1996; White et al. 1990), Bt-2a/Bt-2b (Glass and Donaldson 1995) and CAL228F/CAL737R (Carbone and Kohn 1999) primer sets, respectively. Amplicons were sequenced and deposited in GenBank with accession numbers MZ604647 and MZ604648 for ITS, OK634402 and OK634403 for BTUB and OK634404 and OK634405 for CAL. BLASTn search showed that isolates were >99 % (ITS, BTUB and CAL) identical to S. sclerotiorum GenBank accession no. KF859933, CP017815 and KF871381, respectively. Pathogenicity tests were conducted using eight one-year old grapevines cv. Cabernet Sauvignon. Old and new green shoots were inoculated by inserting a 6-mm plug of mycelium taken from actively growing cultures on PDA into cuts made at the base and at the distal part of each shoot with a sterile scalpel with a total of eight inoculation points per plant. Inoculated wounds were sealed with Parafilm tape to avoid rapid dehydration. Inoculated plants and an equal number of wounded but non-inoculated plants (negative controls) were maintained at 25 ± 1°C for 48 h in plastic containers to ensure a high relative humidity (>90%). After 5 days, the infection girdled and rotted the green new shoots, whereas the older partially lignified shoots developed a localized long brown lesion that reached 16 cm in length. Due to the rotting of the basal part of the petiole, leaves turned gray, wilted, and died, easily detaching from the stem. In advanced stages of the disease, 7 days after infection, branches died and fell with the leaves remained attached (Fig 1 A, B). Reisolations from diseased shoots were successfully performed on PDA to fulfill Koch’s postulates. S. slerotiorum was previously reported on grapevine causing shoot blight in Chile (Latorre and Guerrero, 2001), Korea (Jong-Han et al. 2009), California-USA (Boland and Hall, 1994) and Australia (Hall et al. 2002). AlsoS. sclerotiorum was reported among the endophytic mycobiota associated with Vitis vinifera in the Iberian Peninsula (Gonzalez and Tello, 2011) but not as a pathogen causing visible symptoms on that crop. So, this is the first report of the occurrence of S. slerotiorum as a pathogen of grapevines in Spain causing symptoms of canker and shoot blight. This finding highlights a potential risk of this fungal disease for the wine industry in the Mediterranean region and specially for Spain, the country with the largest acreage devoted to grapevines. Although chemical and biological are suitable control strategies, disease management is difficult as sclerotia of Sclerotinia can remain in the soil for up to eight years (Adams and Ayears, 1979), and preventive surveys are greatly recommended as an important epidemiological tool to monitor the epidemiology of disease and identify potential outbreaks of this new pathogen on grapevine in Spain.
Fuente
Posprint del artículo publicado en Plant disease, febrero 2024
Materia
PRODUCCION VEGETAL
SCLEROTINIA SCLEROTIORUM
HONGOS
UVA
VID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/17770

id RIUCA_c14ea2dc3769fc6e63392391d3bd6e05
oai_identifier_str oai:ucacris:123456789/17770
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, SpainPerelló, Analía E.Olmo, DiegoBusquets, AntoniRomero Munar, AntoniaQuetglas, Bàrbara MariaGost, Pere AntoniBerbegal, M.Armengol, J.PRODUCCION VEGETALSCLEROTINIA SCLEROTIORUMHONGOSUVAVIDFil: Perelló, Analía E. Pontificia Universidad Católica Argentina, Facultad de Ingeniería y Ciencias Agrarias. Laboratorio e producción vegetal; ArgentinaFil: Olmo, Diego. Serveis de Millora Agrària i Pesqueral; EspañaFil: Busquets, Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; EspañaFil: Busquets, Antoni. Universitat de les Illes Balears. Consejo Superior de Investigaciones Científicas. Instituto Mediterráneo de Estudios Avanzados; EspañaFil: Romero Munar, Antonia. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; EspañaFil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; EspañaFil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Department of Engineering; EspañaFil: Gost, Pere Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; EspañaFil: Gost, Pere Antoni. Universitat de les Illes Balears. Department of Engineering; EspañaFil: Berbegal, M. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; EspañaFil: Armengol, J. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; EspañaAbstract: In 2021, grapevines (Vitis vinifera L.) cv. Callet growing in a commercial vineyard located at Pollença (northeast of the island of Majorca, Spain) showed severe symptoms of shoot blight during spring and early summer, with an incidence of 70%. Symptoms consisted of elongated cankered-like lesions, surrounded by water-soaked darker tissues, that developed at the base or around the middle nodes of the shoot. For fungal isolation, shoot samples with lesions were collected, surface disinfected with 2% NaCl for 90s, rinsed twice with deionized water and placed in Petri plates containing potato dextrose agar (PDA). The plates were incubated at 25°C under 12 h light-darkness for 6 days. Isolations consistently yielded on kind of fungal colonies that produced white mycelium and black spherical to elongated sclerotia (2 to 10 mm in diameter). Morphological characterization was consistent with the description of Sclerotinia sclerotiorum (Lib.) de Bary (Bolton et al. 2006). Three isolates (UIB 118-1, UIB 118-26, and UIB 129-41) were preserved and deposited in the Culture Collection of Microbiology-Faculty of Sciences, University of Balearic Islands, Spain. Genomic DNA was extracted from isolates UIB 118-26 and UIB 129-41 using the EZNA Miniprep Kit (Omega Bio-Tek, Norcross, GA). The internal transcribed spacer (ITS) region of ribosomal DNA, β-tubulin (BTUB) and calmodulin (CAL) gene regions were amplified using ITS1F-ITS4 (Gardes and Bruns, 1996; White et al. 1990), Bt-2a/Bt-2b (Glass and Donaldson 1995) and CAL228F/CAL737R (Carbone and Kohn 1999) primer sets, respectively. Amplicons were sequenced and deposited in GenBank with accession numbers MZ604647 and MZ604648 for ITS, OK634402 and OK634403 for BTUB and OK634404 and OK634405 for CAL. BLASTn search showed that isolates were >99 % (ITS, BTUB and CAL) identical to S. sclerotiorum GenBank accession no. KF859933, CP017815 and KF871381, respectively. Pathogenicity tests were conducted using eight one-year old grapevines cv. Cabernet Sauvignon. Old and new green shoots were inoculated by inserting a 6-mm plug of mycelium taken from actively growing cultures on PDA into cuts made at the base and at the distal part of each shoot with a sterile scalpel with a total of eight inoculation points per plant. Inoculated wounds were sealed with Parafilm tape to avoid rapid dehydration. Inoculated plants and an equal number of wounded but non-inoculated plants (negative controls) were maintained at 25 ± 1°C for 48 h in plastic containers to ensure a high relative humidity (>90%). After 5 days, the infection girdled and rotted the green new shoots, whereas the older partially lignified shoots developed a localized long brown lesion that reached 16 cm in length. Due to the rotting of the basal part of the petiole, leaves turned gray, wilted, and died, easily detaching from the stem. In advanced stages of the disease, 7 days after infection, branches died and fell with the leaves remained attached (Fig 1 A, B). Reisolations from diseased shoots were successfully performed on PDA to fulfill Koch’s postulates. S. slerotiorum was previously reported on grapevine causing shoot blight in Chile (Latorre and Guerrero, 2001), Korea (Jong-Han et al. 2009), California-USA (Boland and Hall, 1994) and Australia (Hall et al. 2002). AlsoS. sclerotiorum was reported among the endophytic mycobiota associated with Vitis vinifera in the Iberian Peninsula (Gonzalez and Tello, 2011) but not as a pathogen causing visible symptoms on that crop. So, this is the first report of the occurrence of S. slerotiorum as a pathogen of grapevines in Spain causing symptoms of canker and shoot blight. This finding highlights a potential risk of this fungal disease for the wine industry in the Mediterranean region and specially for Spain, the country with the largest acreage devoted to grapevines. Although chemical and biological are suitable control strategies, disease management is difficult as sclerotia of Sclerotinia can remain in the soil for up to eight years (Adams and Ayears, 1979), and preventive surveys are greatly recommended as an important epidemiological tool to monitor the epidemiology of disease and identify potential outbreaks of this new pathogen on grapevine in Spain.Sociedad Estadounidense de Fitopatología2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/177700191-2917 (impreso)1943-7692 (on line)10.1094/PDIS-12-23-2570-PDNPerelló, A. E. et al. First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain [en línea]. Posprint del artículo publicado en Plant disease, febrero 2024. doi: 10.1094/PDIS-12-23-2570-PDN. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/17770Posprint del artículo publicado en Plant disease, febrero 2024reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica ArgentinaengInteracción planta-hongos fitopatógenos y benéficos en el contexto de enfermedadEspañainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:59:41Zoai:ucacris:123456789/17770instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:59:41.414Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
title First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
spellingShingle First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
Perelló, Analía E.
PRODUCCION VEGETAL
SCLEROTINIA SCLEROTIORUM
HONGOS
UVA
VID
title_short First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
title_full First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
title_fullStr First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
title_full_unstemmed First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
title_sort First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
dc.creator.none.fl_str_mv Perelló, Analía E.
Olmo, Diego
Busquets, Antoni
Romero Munar, Antonia
Quetglas, Bàrbara Maria
Gost, Pere Antoni
Berbegal, M.
Armengol, J.
author Perelló, Analía E.
author_facet Perelló, Analía E.
Olmo, Diego
Busquets, Antoni
Romero Munar, Antonia
Quetglas, Bàrbara Maria
Gost, Pere Antoni
Berbegal, M.
Armengol, J.
author_role author
author2 Olmo, Diego
Busquets, Antoni
Romero Munar, Antonia
Quetglas, Bàrbara Maria
Gost, Pere Antoni
Berbegal, M.
Armengol, J.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PRODUCCION VEGETAL
SCLEROTINIA SCLEROTIORUM
HONGOS
UVA
VID
topic PRODUCCION VEGETAL
SCLEROTINIA SCLEROTIORUM
HONGOS
UVA
VID
dc.description.none.fl_txt_mv Fil: Perelló, Analía E. Pontificia Universidad Católica Argentina, Facultad de Ingeniería y Ciencias Agrarias. Laboratorio e producción vegetal; Argentina
Fil: Olmo, Diego. Serveis de Millora Agrària i Pesqueral; España
Fil: Busquets, Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Busquets, Antoni. Universitat de les Illes Balears. Consejo Superior de Investigaciones Científicas. Instituto Mediterráneo de Estudios Avanzados; España
Fil: Romero Munar, Antonia. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Department of Engineering; España
Fil: Gost, Pere Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Gost, Pere Antoni. Universitat de les Illes Balears. Department of Engineering; España
Fil: Berbegal, M. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; España
Fil: Armengol, J. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; España
Abstract: In 2021, grapevines (Vitis vinifera L.) cv. Callet growing in a commercial vineyard located at Pollença (northeast of the island of Majorca, Spain) showed severe symptoms of shoot blight during spring and early summer, with an incidence of 70%. Symptoms consisted of elongated cankered-like lesions, surrounded by water-soaked darker tissues, that developed at the base or around the middle nodes of the shoot. For fungal isolation, shoot samples with lesions were collected, surface disinfected with 2% NaCl for 90s, rinsed twice with deionized water and placed in Petri plates containing potato dextrose agar (PDA). The plates were incubated at 25°C under 12 h light-darkness for 6 days. Isolations consistently yielded on kind of fungal colonies that produced white mycelium and black spherical to elongated sclerotia (2 to 10 mm in diameter). Morphological characterization was consistent with the description of Sclerotinia sclerotiorum (Lib.) de Bary (Bolton et al. 2006). Three isolates (UIB 118-1, UIB 118-26, and UIB 129-41) were preserved and deposited in the Culture Collection of Microbiology-Faculty of Sciences, University of Balearic Islands, Spain. Genomic DNA was extracted from isolates UIB 118-26 and UIB 129-41 using the EZNA Miniprep Kit (Omega Bio-Tek, Norcross, GA). The internal transcribed spacer (ITS) region of ribosomal DNA, β-tubulin (BTUB) and calmodulin (CAL) gene regions were amplified using ITS1F-ITS4 (Gardes and Bruns, 1996; White et al. 1990), Bt-2a/Bt-2b (Glass and Donaldson 1995) and CAL228F/CAL737R (Carbone and Kohn 1999) primer sets, respectively. Amplicons were sequenced and deposited in GenBank with accession numbers MZ604647 and MZ604648 for ITS, OK634402 and OK634403 for BTUB and OK634404 and OK634405 for CAL. BLASTn search showed that isolates were >99 % (ITS, BTUB and CAL) identical to S. sclerotiorum GenBank accession no. KF859933, CP017815 and KF871381, respectively. Pathogenicity tests were conducted using eight one-year old grapevines cv. Cabernet Sauvignon. Old and new green shoots were inoculated by inserting a 6-mm plug of mycelium taken from actively growing cultures on PDA into cuts made at the base and at the distal part of each shoot with a sterile scalpel with a total of eight inoculation points per plant. Inoculated wounds were sealed with Parafilm tape to avoid rapid dehydration. Inoculated plants and an equal number of wounded but non-inoculated plants (negative controls) were maintained at 25 ± 1°C for 48 h in plastic containers to ensure a high relative humidity (>90%). After 5 days, the infection girdled and rotted the green new shoots, whereas the older partially lignified shoots developed a localized long brown lesion that reached 16 cm in length. Due to the rotting of the basal part of the petiole, leaves turned gray, wilted, and died, easily detaching from the stem. In advanced stages of the disease, 7 days after infection, branches died and fell with the leaves remained attached (Fig 1 A, B). Reisolations from diseased shoots were successfully performed on PDA to fulfill Koch’s postulates. S. slerotiorum was previously reported on grapevine causing shoot blight in Chile (Latorre and Guerrero, 2001), Korea (Jong-Han et al. 2009), California-USA (Boland and Hall, 1994) and Australia (Hall et al. 2002). AlsoS. sclerotiorum was reported among the endophytic mycobiota associated with Vitis vinifera in the Iberian Peninsula (Gonzalez and Tello, 2011) but not as a pathogen causing visible symptoms on that crop. So, this is the first report of the occurrence of S. slerotiorum as a pathogen of grapevines in Spain causing symptoms of canker and shoot blight. This finding highlights a potential risk of this fungal disease for the wine industry in the Mediterranean region and specially for Spain, the country with the largest acreage devoted to grapevines. Although chemical and biological are suitable control strategies, disease management is difficult as sclerotia of Sclerotinia can remain in the soil for up to eight years (Adams and Ayears, 1979), and preventive surveys are greatly recommended as an important epidemiological tool to monitor the epidemiology of disease and identify potential outbreaks of this new pathogen on grapevine in Spain.
description Fil: Perelló, Analía E. Pontificia Universidad Católica Argentina, Facultad de Ingeniería y Ciencias Agrarias. Laboratorio e producción vegetal; Argentina
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/17770
0191-2917 (impreso)
1943-7692 (on line)
10.1094/PDIS-12-23-2570-PDN
Perelló, A. E. et al. First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain [en línea]. Posprint del artículo publicado en Plant disease, febrero 2024. doi: 10.1094/PDIS-12-23-2570-PDN. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/17770
url https://repositorio.uca.edu.ar/handle/123456789/17770
identifier_str_mv 0191-2917 (impreso)
1943-7692 (on line)
10.1094/PDIS-12-23-2570-PDN
Perelló, A. E. et al. First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain [en línea]. Posprint del artículo publicado en Plant disease, febrero 2024. doi: 10.1094/PDIS-12-23-2570-PDN. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/17770
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Interacción planta-hongos fitopatógenos y benéficos en el contexto de enfermedad
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv España
dc.publisher.none.fl_str_mv Sociedad Estadounidense de Fitopatología
publisher.none.fl_str_mv Sociedad Estadounidense de Fitopatología
dc.source.none.fl_str_mv Posprint del artículo publicado en Plant disease, febrero 2024
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.13397