First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain
- Autores
- Perelló, Analía E.; Olmo, Diego; Busquets, Antoni; Romero Munar, Antonia; Quetglas, Bàrbara Maria; Gost, Pere Antoni; Berbegal, M.; Armengol, J.
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- Fil: Perelló, Analía E. Pontificia Universidad Católica Argentina, Facultad de Ingeniería y Ciencias Agrarias. Laboratorio e producción vegetal; Argentina
Fil: Olmo, Diego. Serveis de Millora Agrària i Pesqueral; España
Fil: Busquets, Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Busquets, Antoni. Universitat de les Illes Balears. Consejo Superior de Investigaciones Científicas. Instituto Mediterráneo de Estudios Avanzados; España
Fil: Romero Munar, Antonia. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Department of Engineering; España
Fil: Gost, Pere Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España
Fil: Gost, Pere Antoni. Universitat de les Illes Balears. Department of Engineering; España
Fil: Berbegal, M. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; España
Fil: Armengol, J. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; España
Abstract: In 2021, grapevines (Vitis vinifera L.) cv. Callet growing in a commercial vineyard located at Pollença (northeast of the island of Majorca, Spain) showed severe symptoms of shoot blight during spring and early summer, with an incidence of 70%. Symptoms consisted of elongated cankered-like lesions, surrounded by water-soaked darker tissues, that developed at the base or around the middle nodes of the shoot. For fungal isolation, shoot samples with lesions were collected, surface disinfected with 2% NaCl for 90s, rinsed twice with deionized water and placed in Petri plates containing potato dextrose agar (PDA). The plates were incubated at 25°C under 12 h light-darkness for 6 days. Isolations consistently yielded on kind of fungal colonies that produced white mycelium and black spherical to elongated sclerotia (2 to 10 mm in diameter). Morphological characterization was consistent with the description of Sclerotinia sclerotiorum (Lib.) de Bary (Bolton et al. 2006). Three isolates (UIB 118-1, UIB 118-26, and UIB 129-41) were preserved and deposited in the Culture Collection of Microbiology-Faculty of Sciences, University of Balearic Islands, Spain. Genomic DNA was extracted from isolates UIB 118-26 and UIB 129-41 using the EZNA Miniprep Kit (Omega Bio-Tek, Norcross, GA). The internal transcribed spacer (ITS) region of ribosomal DNA, β-tubulin (BTUB) and calmodulin (CAL) gene regions were amplified using ITS1F-ITS4 (Gardes and Bruns, 1996; White et al. 1990), Bt-2a/Bt-2b (Glass and Donaldson 1995) and CAL228F/CAL737R (Carbone and Kohn 1999) primer sets, respectively. Amplicons were sequenced and deposited in GenBank with accession numbers MZ604647 and MZ604648 for ITS, OK634402 and OK634403 for BTUB and OK634404 and OK634405 for CAL. BLASTn search showed that isolates were >99 % (ITS, BTUB and CAL) identical to S. sclerotiorum GenBank accession no. KF859933, CP017815 and KF871381, respectively. Pathogenicity tests were conducted using eight one-year old grapevines cv. Cabernet Sauvignon. Old and new green shoots were inoculated by inserting a 6-mm plug of mycelium taken from actively growing cultures on PDA into cuts made at the base and at the distal part of each shoot with a sterile scalpel with a total of eight inoculation points per plant. Inoculated wounds were sealed with Parafilm tape to avoid rapid dehydration. Inoculated plants and an equal number of wounded but non-inoculated plants (negative controls) were maintained at 25 ± 1°C for 48 h in plastic containers to ensure a high relative humidity (>90%). After 5 days, the infection girdled and rotted the green new shoots, whereas the older partially lignified shoots developed a localized long brown lesion that reached 16 cm in length. Due to the rotting of the basal part of the petiole, leaves turned gray, wilted, and died, easily detaching from the stem. In advanced stages of the disease, 7 days after infection, branches died and fell with the leaves remained attached (Fig 1 A, B). Reisolations from diseased shoots were successfully performed on PDA to fulfill Koch’s postulates. S. slerotiorum was previously reported on grapevine causing shoot blight in Chile (Latorre and Guerrero, 2001), Korea (Jong-Han et al. 2009), California-USA (Boland and Hall, 1994) and Australia (Hall et al. 2002). AlsoS. sclerotiorum was reported among the endophytic mycobiota associated with Vitis vinifera in the Iberian Peninsula (Gonzalez and Tello, 2011) but not as a pathogen causing visible symptoms on that crop. So, this is the first report of the occurrence of S. slerotiorum as a pathogen of grapevines in Spain causing symptoms of canker and shoot blight. This finding highlights a potential risk of this fungal disease for the wine industry in the Mediterranean region and specially for Spain, the country with the largest acreage devoted to grapevines. Although chemical and biological are suitable control strategies, disease management is difficult as sclerotia of Sclerotinia can remain in the soil for up to eight years (Adams and Ayears, 1979), and preventive surveys are greatly recommended as an important epidemiological tool to monitor the epidemiology of disease and identify potential outbreaks of this new pathogen on grapevine in Spain. - Fuente
- Posprint del artículo publicado en Plant disease, febrero 2024
- Materia
-
PRODUCCION VEGETAL
SCLEROTINIA SCLEROTIORUM
HONGOS
UVA
VID - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Pontificia Universidad Católica Argentina
- OAI Identificador
- oai:ucacris:123456789/17770
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First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, SpainPerelló, Analía E.Olmo, DiegoBusquets, AntoniRomero Munar, AntoniaQuetglas, Bàrbara MariaGost, Pere AntoniBerbegal, M.Armengol, J.PRODUCCION VEGETALSCLEROTINIA SCLEROTIORUMHONGOSUVAVIDFil: Perelló, Analía E. Pontificia Universidad Católica Argentina, Facultad de Ingeniería y Ciencias Agrarias. Laboratorio e producción vegetal; ArgentinaFil: Olmo, Diego. Serveis de Millora Agrària i Pesqueral; EspañaFil: Busquets, Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; EspañaFil: Busquets, Antoni. Universitat de les Illes Balears. Consejo Superior de Investigaciones Científicas. Instituto Mediterráneo de Estudios Avanzados; EspañaFil: Romero Munar, Antonia. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; EspañaFil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; EspañaFil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Department of Engineering; EspañaFil: Gost, Pere Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; EspañaFil: Gost, Pere Antoni. Universitat de les Illes Balears. Department of Engineering; EspañaFil: Berbegal, M. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; EspañaFil: Armengol, J. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; EspañaAbstract: In 2021, grapevines (Vitis vinifera L.) cv. Callet growing in a commercial vineyard located at Pollença (northeast of the island of Majorca, Spain) showed severe symptoms of shoot blight during spring and early summer, with an incidence of 70%. Symptoms consisted of elongated cankered-like lesions, surrounded by water-soaked darker tissues, that developed at the base or around the middle nodes of the shoot. For fungal isolation, shoot samples with lesions were collected, surface disinfected with 2% NaCl for 90s, rinsed twice with deionized water and placed in Petri plates containing potato dextrose agar (PDA). The plates were incubated at 25°C under 12 h light-darkness for 6 days. Isolations consistently yielded on kind of fungal colonies that produced white mycelium and black spherical to elongated sclerotia (2 to 10 mm in diameter). Morphological characterization was consistent with the description of Sclerotinia sclerotiorum (Lib.) de Bary (Bolton et al. 2006). Three isolates (UIB 118-1, UIB 118-26, and UIB 129-41) were preserved and deposited in the Culture Collection of Microbiology-Faculty of Sciences, University of Balearic Islands, Spain. Genomic DNA was extracted from isolates UIB 118-26 and UIB 129-41 using the EZNA Miniprep Kit (Omega Bio-Tek, Norcross, GA). The internal transcribed spacer (ITS) region of ribosomal DNA, β-tubulin (BTUB) and calmodulin (CAL) gene regions were amplified using ITS1F-ITS4 (Gardes and Bruns, 1996; White et al. 1990), Bt-2a/Bt-2b (Glass and Donaldson 1995) and CAL228F/CAL737R (Carbone and Kohn 1999) primer sets, respectively. Amplicons were sequenced and deposited in GenBank with accession numbers MZ604647 and MZ604648 for ITS, OK634402 and OK634403 for BTUB and OK634404 and OK634405 for CAL. BLASTn search showed that isolates were >99 % (ITS, BTUB and CAL) identical to S. sclerotiorum GenBank accession no. KF859933, CP017815 and KF871381, respectively. Pathogenicity tests were conducted using eight one-year old grapevines cv. Cabernet Sauvignon. Old and new green shoots were inoculated by inserting a 6-mm plug of mycelium taken from actively growing cultures on PDA into cuts made at the base and at the distal part of each shoot with a sterile scalpel with a total of eight inoculation points per plant. Inoculated wounds were sealed with Parafilm tape to avoid rapid dehydration. Inoculated plants and an equal number of wounded but non-inoculated plants (negative controls) were maintained at 25 ± 1°C for 48 h in plastic containers to ensure a high relative humidity (>90%). After 5 days, the infection girdled and rotted the green new shoots, whereas the older partially lignified shoots developed a localized long brown lesion that reached 16 cm in length. Due to the rotting of the basal part of the petiole, leaves turned gray, wilted, and died, easily detaching from the stem. In advanced stages of the disease, 7 days after infection, branches died and fell with the leaves remained attached (Fig 1 A, B). Reisolations from diseased shoots were successfully performed on PDA to fulfill Koch’s postulates. S. slerotiorum was previously reported on grapevine causing shoot blight in Chile (Latorre and Guerrero, 2001), Korea (Jong-Han et al. 2009), California-USA (Boland and Hall, 1994) and Australia (Hall et al. 2002). AlsoS. sclerotiorum was reported among the endophytic mycobiota associated with Vitis vinifera in the Iberian Peninsula (Gonzalez and Tello, 2011) but not as a pathogen causing visible symptoms on that crop. So, this is the first report of the occurrence of S. slerotiorum as a pathogen of grapevines in Spain causing symptoms of canker and shoot blight. This finding highlights a potential risk of this fungal disease for the wine industry in the Mediterranean region and specially for Spain, the country with the largest acreage devoted to grapevines. Although chemical and biological are suitable control strategies, disease management is difficult as sclerotia of Sclerotinia can remain in the soil for up to eight years (Adams and Ayears, 1979), and preventive surveys are greatly recommended as an important epidemiological tool to monitor the epidemiology of disease and identify potential outbreaks of this new pathogen on grapevine in Spain.Sociedad Estadounidense de Fitopatología2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/177700191-2917 (impreso)1943-7692 (on line)10.1094/PDIS-12-23-2570-PDNPerelló, A. E. et al. First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain [en línea]. Posprint del artículo publicado en Plant disease, febrero 2024. doi: 10.1094/PDIS-12-23-2570-PDN. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/17770Posprint del artículo publicado en Plant disease, febrero 2024reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica ArgentinaengInteracción planta-hongos fitopatógenos y benéficos en el contexto de enfermedadEspañainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:59:41Zoai:ucacris:123456789/17770instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:59:41.414Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse |
dc.title.none.fl_str_mv |
First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain |
title |
First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain |
spellingShingle |
First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain Perelló, Analía E. PRODUCCION VEGETAL SCLEROTINIA SCLEROTIORUM HONGOS UVA VID |
title_short |
First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain |
title_full |
First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain |
title_fullStr |
First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain |
title_full_unstemmed |
First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain |
title_sort |
First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain |
dc.creator.none.fl_str_mv |
Perelló, Analía E. Olmo, Diego Busquets, Antoni Romero Munar, Antonia Quetglas, Bàrbara Maria Gost, Pere Antoni Berbegal, M. Armengol, J. |
author |
Perelló, Analía E. |
author_facet |
Perelló, Analía E. Olmo, Diego Busquets, Antoni Romero Munar, Antonia Quetglas, Bàrbara Maria Gost, Pere Antoni Berbegal, M. Armengol, J. |
author_role |
author |
author2 |
Olmo, Diego Busquets, Antoni Romero Munar, Antonia Quetglas, Bàrbara Maria Gost, Pere Antoni Berbegal, M. Armengol, J. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
PRODUCCION VEGETAL SCLEROTINIA SCLEROTIORUM HONGOS UVA VID |
topic |
PRODUCCION VEGETAL SCLEROTINIA SCLEROTIORUM HONGOS UVA VID |
dc.description.none.fl_txt_mv |
Fil: Perelló, Analía E. Pontificia Universidad Católica Argentina, Facultad de Ingeniería y Ciencias Agrarias. Laboratorio e producción vegetal; Argentina Fil: Olmo, Diego. Serveis de Millora Agrària i Pesqueral; España Fil: Busquets, Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España Fil: Busquets, Antoni. Universitat de les Illes Balears. Consejo Superior de Investigaciones Científicas. Instituto Mediterráneo de Estudios Avanzados; España Fil: Romero Munar, Antonia. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España Fil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España Fil: Quetglas, Bàrbara Maria. Universitat de les Illes Balears. Department of Engineering; España Fil: Gost, Pere Antoni. Universitat de les Illes Balears. Facultat de Ciencies. Departament de Biologia; España Fil: Gost, Pere Antoni. Universitat de les Illes Balears. Department of Engineering; España Fil: Berbegal, M. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; España Fil: Armengol, J. Universitat Politècnica de València. Instituto Agroforestal Mediterráneo; España Abstract: In 2021, grapevines (Vitis vinifera L.) cv. Callet growing in a commercial vineyard located at Pollença (northeast of the island of Majorca, Spain) showed severe symptoms of shoot blight during spring and early summer, with an incidence of 70%. Symptoms consisted of elongated cankered-like lesions, surrounded by water-soaked darker tissues, that developed at the base or around the middle nodes of the shoot. For fungal isolation, shoot samples with lesions were collected, surface disinfected with 2% NaCl for 90s, rinsed twice with deionized water and placed in Petri plates containing potato dextrose agar (PDA). The plates were incubated at 25°C under 12 h light-darkness for 6 days. Isolations consistently yielded on kind of fungal colonies that produced white mycelium and black spherical to elongated sclerotia (2 to 10 mm in diameter). Morphological characterization was consistent with the description of Sclerotinia sclerotiorum (Lib.) de Bary (Bolton et al. 2006). Three isolates (UIB 118-1, UIB 118-26, and UIB 129-41) were preserved and deposited in the Culture Collection of Microbiology-Faculty of Sciences, University of Balearic Islands, Spain. Genomic DNA was extracted from isolates UIB 118-26 and UIB 129-41 using the EZNA Miniprep Kit (Omega Bio-Tek, Norcross, GA). The internal transcribed spacer (ITS) region of ribosomal DNA, β-tubulin (BTUB) and calmodulin (CAL) gene regions were amplified using ITS1F-ITS4 (Gardes and Bruns, 1996; White et al. 1990), Bt-2a/Bt-2b (Glass and Donaldson 1995) and CAL228F/CAL737R (Carbone and Kohn 1999) primer sets, respectively. Amplicons were sequenced and deposited in GenBank with accession numbers MZ604647 and MZ604648 for ITS, OK634402 and OK634403 for BTUB and OK634404 and OK634405 for CAL. BLASTn search showed that isolates were >99 % (ITS, BTUB and CAL) identical to S. sclerotiorum GenBank accession no. KF859933, CP017815 and KF871381, respectively. Pathogenicity tests were conducted using eight one-year old grapevines cv. Cabernet Sauvignon. Old and new green shoots were inoculated by inserting a 6-mm plug of mycelium taken from actively growing cultures on PDA into cuts made at the base and at the distal part of each shoot with a sterile scalpel with a total of eight inoculation points per plant. Inoculated wounds were sealed with Parafilm tape to avoid rapid dehydration. Inoculated plants and an equal number of wounded but non-inoculated plants (negative controls) were maintained at 25 ± 1°C for 48 h in plastic containers to ensure a high relative humidity (>90%). After 5 days, the infection girdled and rotted the green new shoots, whereas the older partially lignified shoots developed a localized long brown lesion that reached 16 cm in length. Due to the rotting of the basal part of the petiole, leaves turned gray, wilted, and died, easily detaching from the stem. In advanced stages of the disease, 7 days after infection, branches died and fell with the leaves remained attached (Fig 1 A, B). Reisolations from diseased shoots were successfully performed on PDA to fulfill Koch’s postulates. S. slerotiorum was previously reported on grapevine causing shoot blight in Chile (Latorre and Guerrero, 2001), Korea (Jong-Han et al. 2009), California-USA (Boland and Hall, 1994) and Australia (Hall et al. 2002). AlsoS. sclerotiorum was reported among the endophytic mycobiota associated with Vitis vinifera in the Iberian Peninsula (Gonzalez and Tello, 2011) but not as a pathogen causing visible symptoms on that crop. So, this is the first report of the occurrence of S. slerotiorum as a pathogen of grapevines in Spain causing symptoms of canker and shoot blight. This finding highlights a potential risk of this fungal disease for the wine industry in the Mediterranean region and specially for Spain, the country with the largest acreage devoted to grapevines. Although chemical and biological are suitable control strategies, disease management is difficult as sclerotia of Sclerotinia can remain in the soil for up to eight years (Adams and Ayears, 1979), and preventive surveys are greatly recommended as an important epidemiological tool to monitor the epidemiology of disease and identify potential outbreaks of this new pathogen on grapevine in Spain. |
description |
Fil: Perelló, Analía E. Pontificia Universidad Católica Argentina, Facultad de Ingeniería y Ciencias Agrarias. Laboratorio e producción vegetal; Argentina |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
acceptedVersion |
dc.identifier.none.fl_str_mv |
https://repositorio.uca.edu.ar/handle/123456789/17770 0191-2917 (impreso) 1943-7692 (on line) 10.1094/PDIS-12-23-2570-PDN Perelló, A. E. et al. First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain [en línea]. Posprint del artículo publicado en Plant disease, febrero 2024. doi: 10.1094/PDIS-12-23-2570-PDN. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/17770 |
url |
https://repositorio.uca.edu.ar/handle/123456789/17770 |
identifier_str_mv |
0191-2917 (impreso) 1943-7692 (on line) 10.1094/PDIS-12-23-2570-PDN Perelló, A. E. et al. First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Illes Balears, Mallorca, Spain [en línea]. Posprint del artículo publicado en Plant disease, febrero 2024. doi: 10.1094/PDIS-12-23-2570-PDN. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/17770 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Interacción planta-hongos fitopatógenos y benéficos en el contexto de enfermedad |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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application/pdf |
dc.coverage.none.fl_str_mv |
España |
dc.publisher.none.fl_str_mv |
Sociedad Estadounidense de Fitopatología |
publisher.none.fl_str_mv |
Sociedad Estadounidense de Fitopatología |
dc.source.none.fl_str_mv |
Posprint del artículo publicado en Plant disease, febrero 2024 reponame:Repositorio Institucional (UCA) instname:Pontificia Universidad Católica Argentina |
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Pontificia Universidad Católica Argentina |
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Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina |
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claudia_fernandez@uca.edu.ar |
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