Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production
- Autores
- Álvarez Gaona, Izmari Jasel; Assof, Mariela; Jofré, Viviana Patricia; Combina, Mariana; Ciklic, Iván Francisco
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Álvarez Gaona, Izmari Jasel. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Álvarez Gaona, Izmari Jasel. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Assof, Mariela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Jofré, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Ciklic, Iván Francisco. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza. Laboratorio de Biotecnología; Argentina
Fil: Ciklic, Iván Francisco. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina
Abstract: The use of non-conventional yeast species to obtain interesting flavors and aromas has become a new trend in the fermented beverages industry. Among such species, Brettanomyces bruxellensis (B. bruxellensis) has been reported as capable of producing desirable or at least singular aromas in fermented beverages like beer and wine. However, this yeast can also produce an aromatic defect by producing high concentrations of phenolic compounds like, 4-ethylguaiacol and particularly 4-ethylphenol (4-EP). In the present study, we designed a mutant screening method to isolate B. bruxellensis mutants with reduced 4-EP production. More than 1000 mutants were screened with our olfactory screening method, and after further sensory and chemical analysis we were able to select a B. bruxellensis mutant strain with a significant reduction of 4-EP production (more than threefold) and less phenolic perception. - Fuente
- World Journal of Microbiology and Biotechnology 37(6), Año 2021
- Materia
-
VINOS
CERVEZAS
Brettanomyces bruxellensis
LEVADURAS NO CONVENCIONALES
LEVADURA
BEBIDAS ALCOHOLICAS
4-ethylphenol - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Pontificia Universidad Católica Argentina
- OAI Identificador
- oai:ucacris:123456789/11333
Ver los metadatos del registro completo
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Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol productionÁlvarez Gaona, Izmari JaselAssof, MarielaJofré, Viviana PatriciaCombina, MarianaCiklic, Iván FranciscoVINOSCERVEZASBrettanomyces bruxellensisLEVADURAS NO CONVENCIONALESLEVADURABEBIDAS ALCOHOLICAS4-ethylphenolFil: Álvarez Gaona, Izmari Jasel. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Álvarez Gaona, Izmari Jasel. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Assof, Mariela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Jofré, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Ciklic, Iván Francisco. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza. Laboratorio de Biotecnología; ArgentinaFil: Ciklic, Iván Francisco. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; ArgentinaAbstract: The use of non-conventional yeast species to obtain interesting flavors and aromas has become a new trend in the fermented beverages industry. Among such species, Brettanomyces bruxellensis (B. bruxellensis) has been reported as capable of producing desirable or at least singular aromas in fermented beverages like beer and wine. However, this yeast can also produce an aromatic defect by producing high concentrations of phenolic compounds like, 4-ethylguaiacol and particularly 4-ethylphenol (4-EP). In the present study, we designed a mutant screening method to isolate B. bruxellensis mutants with reduced 4-EP production. More than 1000 mutants were screened with our olfactory screening method, and after further sensory and chemical analysis we were able to select a B. bruxellensis mutant strain with a significant reduction of 4-EP production (more than threefold) and less phenolic perception.Springer Nature2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/113331573-0972 (on line)10.1007/s11274-020-02981-5Álvarez Gaona, I. J., Assof, M., Jofré, V. P., Combina, M., Ciklic, I. F. Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production [en línea] World Journal of Microbiology and Biotechnology. 2021, 37(6) Doi: 10.1007/s11274-020-02981-5. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11333World Journal of Microbiology and Biotechnology 37(6), Año 2021reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:43Zoai:ucacris:123456789/11333instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:43.398Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse |
dc.title.none.fl_str_mv |
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production |
title |
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production |
spellingShingle |
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production Álvarez Gaona, Izmari Jasel VINOS CERVEZAS Brettanomyces bruxellensis LEVADURAS NO CONVENCIONALES LEVADURA BEBIDAS ALCOHOLICAS 4-ethylphenol |
title_short |
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production |
title_full |
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production |
title_fullStr |
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production |
title_full_unstemmed |
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production |
title_sort |
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production |
dc.creator.none.fl_str_mv |
Álvarez Gaona, Izmari Jasel Assof, Mariela Jofré, Viviana Patricia Combina, Mariana Ciklic, Iván Francisco |
author |
Álvarez Gaona, Izmari Jasel |
author_facet |
Álvarez Gaona, Izmari Jasel Assof, Mariela Jofré, Viviana Patricia Combina, Mariana Ciklic, Iván Francisco |
author_role |
author |
author2 |
Assof, Mariela Jofré, Viviana Patricia Combina, Mariana Ciklic, Iván Francisco |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
VINOS CERVEZAS Brettanomyces bruxellensis LEVADURAS NO CONVENCIONALES LEVADURA BEBIDAS ALCOHOLICAS 4-ethylphenol |
topic |
VINOS CERVEZAS Brettanomyces bruxellensis LEVADURAS NO CONVENCIONALES LEVADURA BEBIDAS ALCOHOLICAS 4-ethylphenol |
dc.description.none.fl_txt_mv |
Fil: Álvarez Gaona, Izmari Jasel. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina Fil: Álvarez Gaona, Izmari Jasel. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina Fil: Assof, Mariela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina Fil: Jofré, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina Fil: Ciklic, Iván Francisco. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza. Laboratorio de Biotecnología; Argentina Fil: Ciklic, Iván Francisco. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina Abstract: The use of non-conventional yeast species to obtain interesting flavors and aromas has become a new trend in the fermented beverages industry. Among such species, Brettanomyces bruxellensis (B. bruxellensis) has been reported as capable of producing desirable or at least singular aromas in fermented beverages like beer and wine. However, this yeast can also produce an aromatic defect by producing high concentrations of phenolic compounds like, 4-ethylguaiacol and particularly 4-ethylphenol (4-EP). In the present study, we designed a mutant screening method to isolate B. bruxellensis mutants with reduced 4-EP production. More than 1000 mutants were screened with our olfactory screening method, and after further sensory and chemical analysis we were able to select a B. bruxellensis mutant strain with a significant reduction of 4-EP production (more than threefold) and less phenolic perception. |
description |
Fil: Álvarez Gaona, Izmari Jasel. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Mendoza; Argentina |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://repositorio.uca.edu.ar/handle/123456789/11333 1573-0972 (on line) 10.1007/s11274-020-02981-5 Álvarez Gaona, I. J., Assof, M., Jofré, V. P., Combina, M., Ciklic, I. F. Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production [en línea] World Journal of Microbiology and Biotechnology. 2021, 37(6) Doi: 10.1007/s11274-020-02981-5. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11333 |
url |
https://repositorio.uca.edu.ar/handle/123456789/11333 |
identifier_str_mv |
1573-0972 (on line) 10.1007/s11274-020-02981-5 Álvarez Gaona, I. J., Assof, M., Jofré, V. P., Combina, M., Ciklic, I. F. Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4‑ethylphenol production [en línea] World Journal of Microbiology and Biotechnology. 2021, 37(6) Doi: 10.1007/s11274-020-02981-5. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11333 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
World Journal of Microbiology and Biotechnology 37(6), Año 2021 reponame:Repositorio Institucional (UCA) instname:Pontificia Universidad Católica Argentina |
reponame_str |
Repositorio Institucional (UCA) |
collection |
Repositorio Institucional (UCA) |
instname_str |
Pontificia Universidad Católica Argentina |
repository.name.fl_str_mv |
Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina |
repository.mail.fl_str_mv |
claudia_fernandez@uca.edu.ar |
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1836638354879283200 |
score |
13.13397 |