Measurement of melatonin in body fluids : standards, protocols, and procedures

• Autores: Alves de Almeida, Eduardo; Di Mascio, Paolo; Harumi, Tatsuo; Moscovitch, Adam; Hardeland, Rüdiger; Cardinali, Daniel Pedro; Brown, Gregory M.; Pandi Perumal, Seithikurippu R.
• Año de publicación: 2011
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Child's nervous system. 2011, 27 (6)
• Materia: MELATONINA; RITMO CIRCADIANO; ELECTROFORESIS CAPILAR; ESPECTOMETRIA DE MASAS; RADIOINMUNOENSAYO; CROMATOGRAFIA LIQUIDA
• Institución: Pontificia Universidad Católica Argentina

Fil: Alves de Almeida, Eduardo. Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. 1 Departamento de Química e Ciências Ambientais; Brasil
Fil: Di Mascio, Paolo. Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica; Brasil
Fil: Harumi, Tatsuo. Asahikawa Medical College. Department of Anatomy; Japón
Fil: Moscovitch, Adam. Canadian Sleep Institute; Canadá
Fil: Hardeland, Rüdiger. University of Göttingen. Johann Friedrich Blumenbach Institute of Zoology and Anthropology; Alemania
Fil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Medicina. Departamento de Docencia e Investigación; Argentina
Fil: Brown, Gregory M. University of Toronto. Department of Psychiatry; Canadá
Fil: Pandi Perumal, Seithikurippu R. Somnogen Inc; Estados Unidos
Abstract: The circadian rhythm of melatonin in saliva or plasma, or of the melatonin metabolite 6‐ sulphatoxymelatonin in urine, is a defining feature of suprachiasmatic nucleus function, the endogenous oscillatory pacemaker. These measurements are useful to evaluate problems related to the onset or offset of sleep and for assessing phase delays or advances of rhythms in entrained individuals. Additionally, they have become an important tool for psychiatric diagnosis, its use being recommended for phase typing in patients suffering from sleep and mood disorders. Thus, the development of sensitive and selective methods for the precise detection of melatonin in tissues and fluids of animals emerges as necessary. Due to its low concentration and the co‐existence of many other endogenous compounds in blood, the determination of melatonin has been an analytical challenge. This review discusses current methodologies employed for detection and quantification of melatonin in biological fluids and tissues.