Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
- Autores
- Herrera, María Inés; Udovin, Lucas Daniel; Toro-Urrego, Nicolás; Kusnier, Carlos Federico; Luaces, Juan P.; Capani, Francisco
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Herrera, María I. Universidad Católica Argentina. Facultad de Psicología y Psicopedagogía. Centro de Investigaciones en Psicología y Psicopedagogía; Argentina
Fil: Udovin, Lucas Daniel. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Udovin, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Toro-Urrego, Nicolás. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Toro-Urrego, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kusnier, Carlos. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Kusnier, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luaces, Juan P. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Luaces, Juan P. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capani, Francisco. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capani, Francisco. Universidad Católica Argentina. Facultad de Medicina; Argentina
Fil: Capani, Francisco. Universidad Autónoma de Chile; Chile
Abstract: Perinatal asphyxia (PA) is an obstetric complication associated with an impaired gas exchange. This health problem continues to be a determinant of neonatal mortality and neurodevelopmental disorders. Palmitoylethanolamide (PEA) has exerted neuroprotection in several models of brain injury and neurodegeneration. We aimed at evaluating the potential neuroprotective role of PEA in an experimental model, which induces PA in the immature rat brain. PA was induced by placing Sprague Dawley newborn rats in a water bath at 37°C for 19 min. Once their physiological conditions improved, they were given to surrogate mothers that had delivered normally within the last 24 h. The control group was represented by non-fostered vaginally delivered pups, mimicking the clinical situation. Treatment with PEA (10 mg/kg) was administered within the first hour of life. Modifications in the hippocampus were analyzed with conventional electron microscopy, immunohistochemistry (for NeuN, pNF-H/M, MAP-2, and GFAP) and western blot (for pNF H/M, MAP-2, and GFAP). Behavior was also studied throughout Open Field (OF) Test, Passive Avoidance (PA) Task and Elevated Plus Maze (EPM) Test. After 1 month of the PA insult, we observed neuronal nucleus degeneration in CA1 using electron microscopy. Immunohistochemistry revealed a significant increase in pNF-H/M and decrease in MAP-2 in CA1 reactive area. These changes were also observed when analyzing the level of expression of these markers by western blot. Vertical exploration impairments and anxiety-related behaviors were encountered in the OF and EPM tests. PEA treatment attenuated PA-induced hippocampal damage and its corresponding behavioral alterations. These results contribute to the elucidation of PEA neuroprotective role after PA and the future establishment of therapeutic strategies for the developing brain. - Fuente
- Frontiers in Neuroscience, 12:145, 2018
- Materia
-
COMPORTAMIENTO
ASFIXIA PRENATAL
NEUROPSICOLOGIA
TRASTORNOS DEL NEURODESARROLLO - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Pontificia Universidad Católica Argentina
- OAI Identificador
- oai:ucacris:123456789/6220
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Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brainHerrera, María InésUdovin, Lucas DanielToro-Urrego, NicolásKusnier, Carlos FedericoLuaces, Juan P.Capani, FranciscoCOMPORTAMIENTOASFIXIA PRENATALNEUROPSICOLOGIATRASTORNOS DEL NEURODESARROLLOFil: Herrera, María I. Universidad Católica Argentina. Facultad de Psicología y Psicopedagogía. Centro de Investigaciones en Psicología y Psicopedagogía; ArgentinaFil: Udovin, Lucas Daniel. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Udovin, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Toro-Urrego, Nicolás. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Toro-Urrego, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kusnier, Carlos. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Kusnier, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luaces, Juan P. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Luaces, Juan P. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capani, Francisco. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capani, Francisco. Universidad Católica Argentina. Facultad de Medicina; ArgentinaFil: Capani, Francisco. Universidad Autónoma de Chile; ChileAbstract: Perinatal asphyxia (PA) is an obstetric complication associated with an impaired gas exchange. This health problem continues to be a determinant of neonatal mortality and neurodevelopmental disorders. Palmitoylethanolamide (PEA) has exerted neuroprotection in several models of brain injury and neurodegeneration. We aimed at evaluating the potential neuroprotective role of PEA in an experimental model, which induces PA in the immature rat brain. PA was induced by placing Sprague Dawley newborn rats in a water bath at 37°C for 19 min. Once their physiological conditions improved, they were given to surrogate mothers that had delivered normally within the last 24 h. The control group was represented by non-fostered vaginally delivered pups, mimicking the clinical situation. Treatment with PEA (10 mg/kg) was administered within the first hour of life. Modifications in the hippocampus were analyzed with conventional electron microscopy, immunohistochemistry (for NeuN, pNF-H/M, MAP-2, and GFAP) and western blot (for pNF H/M, MAP-2, and GFAP). Behavior was also studied throughout Open Field (OF) Test, Passive Avoidance (PA) Task and Elevated Plus Maze (EPM) Test. After 1 month of the PA insult, we observed neuronal nucleus degeneration in CA1 using electron microscopy. Immunohistochemistry revealed a significant increase in pNF-H/M and decrease in MAP-2 in CA1 reactive area. These changes were also observed when analyzing the level of expression of these markers by western blot. Vertical exploration impairments and anxiety-related behaviors were encountered in the OF and EPM tests. PEA treatment attenuated PA-induced hippocampal damage and its corresponding behavioral alterations. These results contribute to the elucidation of PEA neuroprotective role after PA and the future establishment of therapeutic strategies for the developing brain.Frontiers Media2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/62201662-453XHerrera, M. I., Udovin, L. D., Toro-Urrego, N., Kusnier, C. F., Luaces, J. P., y Capani, F. (2018). Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain [en línea] Frontiers in Neuroscience, 12:145. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/6220Frontiers in Neuroscience, 12:145, 2018reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:56:18Zoai:ucacris:123456789/6220instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:56:18.922Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse |
dc.title.none.fl_str_mv |
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain |
title |
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain |
spellingShingle |
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain Herrera, María Inés COMPORTAMIENTO ASFIXIA PRENATAL NEUROPSICOLOGIA TRASTORNOS DEL NEURODESARROLLO |
title_short |
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain |
title_full |
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain |
title_fullStr |
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain |
title_full_unstemmed |
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain |
title_sort |
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain |
dc.creator.none.fl_str_mv |
Herrera, María Inés Udovin, Lucas Daniel Toro-Urrego, Nicolás Kusnier, Carlos Federico Luaces, Juan P. Capani, Francisco |
author |
Herrera, María Inés |
author_facet |
Herrera, María Inés Udovin, Lucas Daniel Toro-Urrego, Nicolás Kusnier, Carlos Federico Luaces, Juan P. Capani, Francisco |
author_role |
author |
author2 |
Udovin, Lucas Daniel Toro-Urrego, Nicolás Kusnier, Carlos Federico Luaces, Juan P. Capani, Francisco |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
COMPORTAMIENTO ASFIXIA PRENATAL NEUROPSICOLOGIA TRASTORNOS DEL NEURODESARROLLO |
topic |
COMPORTAMIENTO ASFIXIA PRENATAL NEUROPSICOLOGIA TRASTORNOS DEL NEURODESARROLLO |
dc.description.none.fl_txt_mv |
Fil: Herrera, María I. Universidad Católica Argentina. Facultad de Psicología y Psicopedagogía. Centro de Investigaciones en Psicología y Psicopedagogía; Argentina Fil: Udovin, Lucas Daniel. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina Fil: Udovin, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Toro-Urrego, Nicolás. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina Fil: Toro-Urrego, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kusnier, Carlos. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina Fil: Kusnier, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Luaces, Juan P. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina Fil: Luaces, Juan P. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Capani, Francisco. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Capani, Francisco. Universidad Católica Argentina. Facultad de Medicina; Argentina Fil: Capani, Francisco. Universidad Autónoma de Chile; Chile Abstract: Perinatal asphyxia (PA) is an obstetric complication associated with an impaired gas exchange. This health problem continues to be a determinant of neonatal mortality and neurodevelopmental disorders. Palmitoylethanolamide (PEA) has exerted neuroprotection in several models of brain injury and neurodegeneration. We aimed at evaluating the potential neuroprotective role of PEA in an experimental model, which induces PA in the immature rat brain. PA was induced by placing Sprague Dawley newborn rats in a water bath at 37°C for 19 min. Once their physiological conditions improved, they were given to surrogate mothers that had delivered normally within the last 24 h. The control group was represented by non-fostered vaginally delivered pups, mimicking the clinical situation. Treatment with PEA (10 mg/kg) was administered within the first hour of life. Modifications in the hippocampus were analyzed with conventional electron microscopy, immunohistochemistry (for NeuN, pNF-H/M, MAP-2, and GFAP) and western blot (for pNF H/M, MAP-2, and GFAP). Behavior was also studied throughout Open Field (OF) Test, Passive Avoidance (PA) Task and Elevated Plus Maze (EPM) Test. After 1 month of the PA insult, we observed neuronal nucleus degeneration in CA1 using electron microscopy. Immunohistochemistry revealed a significant increase in pNF-H/M and decrease in MAP-2 in CA1 reactive area. These changes were also observed when analyzing the level of expression of these markers by western blot. Vertical exploration impairments and anxiety-related behaviors were encountered in the OF and EPM tests. PEA treatment attenuated PA-induced hippocampal damage and its corresponding behavioral alterations. These results contribute to the elucidation of PEA neuroprotective role after PA and the future establishment of therapeutic strategies for the developing brain. |
description |
Fil: Herrera, María I. Universidad Católica Argentina. Facultad de Psicología y Psicopedagogía. Centro de Investigaciones en Psicología y Psicopedagogía; Argentina |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://repositorio.uca.edu.ar/handle/123456789/6220 1662-453X Herrera, M. I., Udovin, L. D., Toro-Urrego, N., Kusnier, C. F., Luaces, J. P., y Capani, F. (2018). Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain [en línea] Frontiers in Neuroscience, 12:145. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/6220 |
url |
https://repositorio.uca.edu.ar/handle/123456789/6220 |
identifier_str_mv |
1662-453X Herrera, M. I., Udovin, L. D., Toro-Urrego, N., Kusnier, C. F., Luaces, J. P., y Capani, F. (2018). Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain [en línea] Frontiers in Neuroscience, 12:145. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/6220 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
Frontiers in Neuroscience, 12:145, 2018 reponame:Repositorio Institucional (UCA) instname:Pontificia Universidad Católica Argentina |
reponame_str |
Repositorio Institucional (UCA) |
collection |
Repositorio Institucional (UCA) |
instname_str |
Pontificia Universidad Católica Argentina |
repository.name.fl_str_mv |
Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina |
repository.mail.fl_str_mv |
claudia_fernandez@uca.edu.ar |
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1836638341795151872 |
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13.070432 |