Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil

Autores
Loureiro, Dana Belén; Olivera, Camila; Tondo, María Laura; Herrero, María Sol; Salvatierra, Lucas Matías; Pérez, Leonardo Martín
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Fil: Loureiro, Dana Belén. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Loureiro, Dana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Loureiro, Dana Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Olivera, Camila. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Olivera, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tondo, María Laura. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Tondo, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tondo, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Herrero, María Sol. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Herrero, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvatierra, Lucas Matías. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Salvatierra, Lucas Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, Leonardo Martín. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Pérez, Leonardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, Leonardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Abstract: Biodegradation of commercial B10 diesel oil (DB10) by the microbial consortium present in a biogas residual sludge was first investigated. Maximum removal efficiencies of the petrochemical and fatty acids methyl ester (FAME) fractions of DB10 were 55 ± 3% and 94 ± 6%, respectively. Taxonomic profiling of the microbial consortium present in the residual sludge was carried out in order to identify potential hydrocarbon-degrading bacteria. After mild selection pressure using DB10, three Pseudomonas species (P. aeruginosa, P. stutzeri and P. mendocina) were mostly found in the treated sample. However, only P. aeruginosa and P. stutzeri showed a rapid growth and high dehydrogenase activity at the expense of DB10 as the sole carbon source. In addition, both strains removed 42 ± 5% and 53 ± 2% of the petrochemical fraction, and >75% of the FAME fraction, respectively. Similar results were found with a bacterial consortium of the three isolated Pseudomonas, indicating no synergistic hydrocarbon degradation by these strains. As expected, biosurfactant production by the pseudomonads was directly associated with the bacterial DB10 biodegradation performance. These results are the first describing petroleum-based hydrocarbon biodegradation ability of a crude facultative residual sludge obtained from a biogas facility; and represent a rational first step in order to understand which bacteria in the sludge may act in petroleum-based fuels degradation.
Fuente
Ecological Engineering. 2020, 144
Materia
BIODEGRADACION
DIESEL
HIDROCARBUROS
BIOGAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/10089
Acceder
id RIUCA_6d2d21e5fc95d5303fa7be4235833fdb
oai_identifier_str oai:ucacris:123456789/10089
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oilLoureiro, Dana BelénOlivera, CamilaTondo, María LauraHerrero, María SolSalvatierra, Lucas MatíasPérez, Leonardo MartínBIODEGRADACIONDIESELHIDROCARBUROSBIOGASFil: Loureiro, Dana Belén. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; ArgentinaFil: Loureiro, Dana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Loureiro, Dana Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Olivera, Camila. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; ArgentinaFil: Olivera, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tondo, María Laura. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; ArgentinaFil: Tondo, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tondo, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Herrero, María Sol. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; ArgentinaFil: Herrero, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salvatierra, Lucas Matías. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; ArgentinaFil: Salvatierra, Lucas Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez, Leonardo Martín. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; ArgentinaFil: Pérez, Leonardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez, Leonardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaAbstract: Biodegradation of commercial B10 diesel oil (DB10) by the microbial consortium present in a biogas residual sludge was first investigated. Maximum removal efficiencies of the petrochemical and fatty acids methyl ester (FAME) fractions of DB10 were 55 ± 3% and 94 ± 6%, respectively. Taxonomic profiling of the microbial consortium present in the residual sludge was carried out in order to identify potential hydrocarbon-degrading bacteria. After mild selection pressure using DB10, three Pseudomonas species (P. aeruginosa, P. stutzeri and P. mendocina) were mostly found in the treated sample. However, only P. aeruginosa and P. stutzeri showed a rapid growth and high dehydrogenase activity at the expense of DB10 as the sole carbon source. In addition, both strains removed 42 ± 5% and 53 ± 2% of the petrochemical fraction, and >75% of the FAME fraction, respectively. Similar results were found with a bacterial consortium of the three isolated Pseudomonas, indicating no synergistic hydrocarbon degradation by these strains. As expected, biosurfactant production by the pseudomonads was directly associated with the bacterial DB10 biodegradation performance. These results are the first describing petroleum-based hydrocarbon biodegradation ability of a crude facultative residual sludge obtained from a biogas facility; and represent a rational first step in order to understand which bacteria in the sludge may act in petroleum-based fuels degradation.Elsevier2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/100890925-857410.1016/j.ecoleng.2019.105710Loureiro, D. B. Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil [en línea]. Ecological Engineering. 2020, 144. doi:10.1016/j.ecoleng.2019.105710 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10089Ecological Engineering. 2020, 144reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:23Zoai:ucacris:123456789/10089instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:24.063Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil
title Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil
spellingShingle Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil
Loureiro, Dana Belén
BIODEGRADACION
DIESEL
HIDROCARBUROS
BIOGAS
title_short Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil
title_full Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil
title_fullStr Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil
title_full_unstemmed Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil
title_sort Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil
dc.creator.none.fl_str_mv Loureiro, Dana Belén
Olivera, Camila
Tondo, María Laura
Herrero, María Sol
Salvatierra, Lucas Matías
Pérez, Leonardo Martín
author Loureiro, Dana Belén
author_facet Loureiro, Dana Belén
Olivera, Camila
Tondo, María Laura
Herrero, María Sol
Salvatierra, Lucas Matías
Pérez, Leonardo Martín
author_role author
author2 Olivera, Camila
Tondo, María Laura
Herrero, María Sol
Salvatierra, Lucas Matías
Pérez, Leonardo Martín
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIODEGRADACION
DIESEL
HIDROCARBUROS
BIOGAS
topic BIODEGRADACION
DIESEL
HIDROCARBUROS
BIOGAS
dc.description.none.fl_txt_mv Fil: Loureiro, Dana Belén. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Loureiro, Dana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Loureiro, Dana Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Olivera, Camila. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Olivera, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tondo, María Laura. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Tondo, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tondo, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Herrero, María Sol. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Herrero, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvatierra, Lucas Matías. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Salvatierra, Lucas Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, Leonardo Martín. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
Fil: Pérez, Leonardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, Leonardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Abstract: Biodegradation of commercial B10 diesel oil (DB10) by the microbial consortium present in a biogas residual sludge was first investigated. Maximum removal efficiencies of the petrochemical and fatty acids methyl ester (FAME) fractions of DB10 were 55 ± 3% and 94 ± 6%, respectively. Taxonomic profiling of the microbial consortium present in the residual sludge was carried out in order to identify potential hydrocarbon-degrading bacteria. After mild selection pressure using DB10, three Pseudomonas species (P. aeruginosa, P. stutzeri and P. mendocina) were mostly found in the treated sample. However, only P. aeruginosa and P. stutzeri showed a rapid growth and high dehydrogenase activity at the expense of DB10 as the sole carbon source. In addition, both strains removed 42 ± 5% and 53 ± 2% of the petrochemical fraction, and >75% of the FAME fraction, respectively. Similar results were found with a bacterial consortium of the three isolated Pseudomonas, indicating no synergistic hydrocarbon degradation by these strains. As expected, biosurfactant production by the pseudomonads was directly associated with the bacterial DB10 biodegradation performance. These results are the first describing petroleum-based hydrocarbon biodegradation ability of a crude facultative residual sludge obtained from a biogas facility; and represent a rational first step in order to understand which bacteria in the sludge may act in petroleum-based fuels degradation.
description Fil: Loureiro, Dana Belén. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Grupo de Biotecnología de Materiales y Medioambiente; Argentina
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/10089
0925-8574
10.1016/j.ecoleng.2019.105710
Loureiro, D. B. Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil [en línea]. Ecological Engineering. 2020, 144. doi:10.1016/j.ecoleng.2019.105710 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10089
url https://repositorio.uca.edu.ar/handle/123456789/10089
identifier_str_mv 0925-8574
10.1016/j.ecoleng.2019.105710
Loureiro, D. B. Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil [en línea]. Ecological Engineering. 2020, 144. doi:10.1016/j.ecoleng.2019.105710 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10089
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Ecological Engineering. 2020, 144
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.13397

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