Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice

Autores
Trebucq, Laura Lucía; Lamberti, Melisa Luciana; Rota, Rosana; Borio, Cristina; Bilen, Marcos; Golombek, Diego A.; Plano, Santiago Andrés; Chiesa, Juan José
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Trebucq, Laura Lucía. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Trebucq, Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lamberti, Melisa Luciana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Lamberti, Melisa Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rota, Rosana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Rota, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borio, Cristina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética, Biología Celular y Molecular; Argentina
Fil: Borio, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bilen, Marcos. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética, Biología Celular y Molecular; Argentina
Fil: Bilen, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golombek, Diego Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Golombek, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golombek, Diego Andrés. Universidad de San Andrés. Escuela de Educación; Argentina
Fil: Plano, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Plano, Santiago Andrés. Pontificia Universidad Católica Argentina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Plano, Santiago Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Chiesa, Juan José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Chiesa, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Introduction: The circadian system synchronizes behavior and physiology to the 24-h light– dark (LD) cycle. Timing of food intake and fasting periods provide strong signals for peripheral circadian clocks regulating nutrient assimilation, glucose, and lipid metabolism. Mice under 12h light:12h dark (LD) cycles exhibit behavioral activity and feeding during the dark period, while fasting occurs at rest during light. Disruption of energy metabolism, leading to an increase in body mass, was reported in experimental models of circadian desynchronization. In this work, the effects of chronic advances of the LD cycles (chronic jet-lag protocol, CJL) were studied on the daily homeostasis of energy metabolism and weight gain. Methods: Male C57 mice were subjected to a CJL or LD schedule, measuring IPGTT, insulinemia, microbiome composition and lipidemia. Results: Mice under CJL show behavioral desynchronization and feeding activity distributed similarly at the light and dark hours and, although feeding a similar daily amount of food as compared to controls, show an increase in weight gain. In addition, ad libitum glycemia rhythm was abolished in CJL-subjected mice, showing similar blood glucose values at light and dark. CJL also generated glucose intolerance at dark in an intraperitoneal glucose tolerance test (IPGTT), with increased insulin release at both light and dark periods. Low-density lipoprotein (LDL) cholesterolemia was increased under this condition, but no changes in HDL cholesterolemia were observed. Firmicutes/ Bacteroidetes ratio was analyzed as a marker of circadian disruption of microbiota composition, showing opposite phases at the light and dark when comparing LD vs. CJL. Discussion: Chronic misalignment of feeding/fasting rhythm leads to metabolic disturbances generating nocturnal hyperglycemia, glucose intolerance and hyperinsulinemia in a IPGTT, increased LDL cholesterolemia, and increased weight gain, underscoring the importance of the timing of food consumption with respect to the circadian system for metabolic health.
Fuente
Frontiers in Nutrition. 2023, 10
Materia
RITMO CIRCADIANO
INTOLERANCIA
GLUCOSA
LIPIDOS
NUTRIENTES
AUMENTO DE PESO
METABOLISMO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/16477
Acceder
id RIUCA_656db5573c716c689ef38fcedf084e82
oai_identifier_str oai:ucacris:123456789/16477
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in miceTrebucq, Laura LucíaLamberti, Melisa LucianaRota, RosanaBorio, CristinaBilen, MarcosGolombek, Diego A.Plano, Santiago AndrésChiesa, Juan JoséRITMO CIRCADIANOINTOLERANCIAGLUCOSALIPIDOSNUTRIENTESAUMENTO DE PESOMETABOLISMOFil: Trebucq, Laura Lucía. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Trebucq, Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lamberti, Melisa Luciana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Lamberti, Melisa Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rota, Rosana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Rota, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Borio, Cristina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética, Biología Celular y Molecular; ArgentinaFil: Borio, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bilen, Marcos. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética, Biología Celular y Molecular; ArgentinaFil: Bilen, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Golombek, Diego Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Golombek, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Golombek, Diego Andrés. Universidad de San Andrés. Escuela de Educación; ArgentinaFil: Plano, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Plano, Santiago Andrés. Pontificia Universidad Católica Argentina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Plano, Santiago Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Chiesa, Juan José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Chiesa, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAbstract: Introduction: The circadian system synchronizes behavior and physiology to the 24-h light– dark (LD) cycle. Timing of food intake and fasting periods provide strong signals for peripheral circadian clocks regulating nutrient assimilation, glucose, and lipid metabolism. Mice under 12h light:12h dark (LD) cycles exhibit behavioral activity and feeding during the dark period, while fasting occurs at rest during light. Disruption of energy metabolism, leading to an increase in body mass, was reported in experimental models of circadian desynchronization. In this work, the effects of chronic advances of the LD cycles (chronic jet-lag protocol, CJL) were studied on the daily homeostasis of energy metabolism and weight gain. Methods: Male C57 mice were subjected to a CJL or LD schedule, measuring IPGTT, insulinemia, microbiome composition and lipidemia. Results: Mice under CJL show behavioral desynchronization and feeding activity distributed similarly at the light and dark hours and, although feeding a similar daily amount of food as compared to controls, show an increase in weight gain. In addition, ad libitum glycemia rhythm was abolished in CJL-subjected mice, showing similar blood glucose values at light and dark. CJL also generated glucose intolerance at dark in an intraperitoneal glucose tolerance test (IPGTT), with increased insulin release at both light and dark periods. Low-density lipoprotein (LDL) cholesterolemia was increased under this condition, but no changes in HDL cholesterolemia were observed. Firmicutes/ Bacteroidetes ratio was analyzed as a marker of circadian disruption of microbiota composition, showing opposite phases at the light and dark when comparing LD vs. CJL. Discussion: Chronic misalignment of feeding/fasting rhythm leads to metabolic disturbances generating nocturnal hyperglycemia, glucose intolerance and hyperinsulinemia in a IPGTT, increased LDL cholesterolemia, and increased weight gain, underscoring the importance of the timing of food consumption with respect to the circadian system for metabolic health.Frontiers Media2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/164772296-861X (online)10.3389/fnut.2023.115464737125029Trebucq, L. L. et al. Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice [en línea]. Frontiers in Nutrition. 2023, 10. doi:10.3389/fnut.2023.1154647. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16477Frontiers in Nutrition. 2023, 10reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:59:17Zoai:ucacris:123456789/16477instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:59:18.103Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
spellingShingle Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
Trebucq, Laura Lucía
RITMO CIRCADIANO
INTOLERANCIA
GLUCOSA
LIPIDOS
NUTRIENTES
AUMENTO DE PESO
METABOLISMO
title_short Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_full Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_fullStr Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_full_unstemmed Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_sort Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
dc.creator.none.fl_str_mv Trebucq, Laura Lucía
Lamberti, Melisa Luciana
Rota, Rosana
Borio, Cristina
Bilen, Marcos
Golombek, Diego A.
Plano, Santiago Andrés
Chiesa, Juan José
author Trebucq, Laura Lucía
author_facet Trebucq, Laura Lucía
Lamberti, Melisa Luciana
Rota, Rosana
Borio, Cristina
Bilen, Marcos
Golombek, Diego A.
Plano, Santiago Andrés
Chiesa, Juan José
author_role author
author2 Lamberti, Melisa Luciana
Rota, Rosana
Borio, Cristina
Bilen, Marcos
Golombek, Diego A.
Plano, Santiago Andrés
Chiesa, Juan José
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RITMO CIRCADIANO
INTOLERANCIA
GLUCOSA
LIPIDOS
NUTRIENTES
AUMENTO DE PESO
METABOLISMO
topic RITMO CIRCADIANO
INTOLERANCIA
GLUCOSA
LIPIDOS
NUTRIENTES
AUMENTO DE PESO
METABOLISMO
dc.description.none.fl_txt_mv Fil: Trebucq, Laura Lucía. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Trebucq, Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lamberti, Melisa Luciana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Lamberti, Melisa Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rota, Rosana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Rota, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borio, Cristina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética, Biología Celular y Molecular; Argentina
Fil: Borio, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bilen, Marcos. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética, Biología Celular y Molecular; Argentina
Fil: Bilen, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golombek, Diego Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Golombek, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golombek, Diego Andrés. Universidad de San Andrés. Escuela de Educación; Argentina
Fil: Plano, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Plano, Santiago Andrés. Pontificia Universidad Católica Argentina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Plano, Santiago Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Chiesa, Juan José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Chiesa, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Introduction: The circadian system synchronizes behavior and physiology to the 24-h light– dark (LD) cycle. Timing of food intake and fasting periods provide strong signals for peripheral circadian clocks regulating nutrient assimilation, glucose, and lipid metabolism. Mice under 12h light:12h dark (LD) cycles exhibit behavioral activity and feeding during the dark period, while fasting occurs at rest during light. Disruption of energy metabolism, leading to an increase in body mass, was reported in experimental models of circadian desynchronization. In this work, the effects of chronic advances of the LD cycles (chronic jet-lag protocol, CJL) were studied on the daily homeostasis of energy metabolism and weight gain. Methods: Male C57 mice were subjected to a CJL or LD schedule, measuring IPGTT, insulinemia, microbiome composition and lipidemia. Results: Mice under CJL show behavioral desynchronization and feeding activity distributed similarly at the light and dark hours and, although feeding a similar daily amount of food as compared to controls, show an increase in weight gain. In addition, ad libitum glycemia rhythm was abolished in CJL-subjected mice, showing similar blood glucose values at light and dark. CJL also generated glucose intolerance at dark in an intraperitoneal glucose tolerance test (IPGTT), with increased insulin release at both light and dark periods. Low-density lipoprotein (LDL) cholesterolemia was increased under this condition, but no changes in HDL cholesterolemia were observed. Firmicutes/ Bacteroidetes ratio was analyzed as a marker of circadian disruption of microbiota composition, showing opposite phases at the light and dark when comparing LD vs. CJL. Discussion: Chronic misalignment of feeding/fasting rhythm leads to metabolic disturbances generating nocturnal hyperglycemia, glucose intolerance and hyperinsulinemia in a IPGTT, increased LDL cholesterolemia, and increased weight gain, underscoring the importance of the timing of food consumption with respect to the circadian system for metabolic health.
description Fil: Trebucq, Laura Lucía. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/16477
2296-861X (online)
10.3389/fnut.2023.1154647
37125029
Trebucq, L. L. et al. Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice [en línea]. Frontiers in Nutrition. 2023, 10. doi:10.3389/fnut.2023.1154647. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16477
url https://repositorio.uca.edu.ar/handle/123456789/16477
identifier_str_mv 2296-861X (online)
10.3389/fnut.2023.1154647
37125029
Trebucq, L. L. et al. Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice [en línea]. Frontiers in Nutrition. 2023, 10. doi:10.3389/fnut.2023.1154647. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16477
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Nutrition. 2023, 10
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.22299

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