Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors

Autores
Girardi, Valentina; Tondo, María Laura; Balaban, Cecilia Lucía; Herrero, María Sol; Olivera, Camila; Salvatierra, Lucas Matías
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Girardi, Valentina. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Girardi, Valentina. CONICET; Argentina
Fil: Tondo, María Laura. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Tondo, María Laura. CONICET; Argentina
Fil: Balaban, Cecilia Lucía. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Balaban, Cecilia Lucía. CONICET; Argentina
Fil: Herrero, María Sol. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Olivera, Camila. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Olivera, Camila. CONICET; Argentina
Fil: Salvatierra, Lucas Matías. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Salvatierra, Lucas Matías. CONICET; Argentina
This article presents an original and detailed study of semi continuous lab-scale anaerobic bioreactors subjected to Sulfate Loads Rates (SLRs) >130 mgSO4 2− /day.Lreactor. At these sulfate inputs, hydrogen sulfide yield by bacterial conversion can reach concentrations above 400 mg/L in the liquid phase and more than 25,000 ppm in gas phase, respectively. Both values comprise significant technical challenges in biogas plants as well as health and environmental concerns. In order to control sulfide in liquid phase, two bioreactors were treated with opposite ferric dosing strategies: one underwent five consecutive cycles of shock precipitation of hydrogen sulfide, reducing peak levels from ~400 mg/L to <40 mg/L; the other involved the application of a more continuous and fine-tuned dosing strategy to manage sulfide concentrations across different stepwise levels: 400–300, 300–200, 200–100, and 100 to <10 mg/L. A third bioreactor, without iron addition, stabilized at ~250 mg/L H2S and 10,000 ppm in biogas over 140 days. A detailed sulfur mass balance enabled analysis of sulfate-to-sulfide conversion rates, Henry’s con stants between liquid and gas phases, and Fe3+/S2− molar ratios for both strategies. Values of 0.99 and 0.65 were obtained, matching with the stoichiometry of FeS during shock precipitation and FeS + S0 and/or Fe2S3 in stepwise precipitation, respectively. High-throughput sequencing of the 16S rRNA gene revealed subtle yet significant changes in the microbial community structures within bioreactors subjected to high sulfate loads and iron addition. Furthermore, the authors characterized the late-stage microbial response following methanogenic process inhibition and the cessation of biogas production.
Fuente
Journal of Water Process Engineering. 2025 77
Materia
BIOGAS
SULFATOS
SULFURO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/20830
Acceder
id RIUCA_5a2966175edf953e6accc405fd4417c8
oai_identifier_str oai:ucacris:123456789/20830
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactorsGirardi, ValentinaTondo, María LauraBalaban, Cecilia LucíaHerrero, María SolOlivera, CamilaSalvatierra, Lucas MatíasBIOGASSULFATOSSULFUROFil: Girardi, Valentina. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; ArgentinaFil: Girardi, Valentina. CONICET; ArgentinaFil: Tondo, María Laura. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; ArgentinaFil: Tondo, María Laura. CONICET; ArgentinaFil: Balaban, Cecilia Lucía. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; ArgentinaFil: Balaban, Cecilia Lucía. CONICET; ArgentinaFil: Herrero, María Sol. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; ArgentinaFil: Olivera, Camila. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; ArgentinaFil: Olivera, Camila. CONICET; ArgentinaFil: Salvatierra, Lucas Matías. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; ArgentinaFil: Salvatierra, Lucas Matías. CONICET; ArgentinaThis article presents an original and detailed study of semi continuous lab-scale anaerobic bioreactors subjected to Sulfate Loads Rates (SLRs) >130 mgSO4 2− /day.Lreactor. At these sulfate inputs, hydrogen sulfide yield by bacterial conversion can reach concentrations above 400 mg/L in the liquid phase and more than 25,000 ppm in gas phase, respectively. Both values comprise significant technical challenges in biogas plants as well as health and environmental concerns. In order to control sulfide in liquid phase, two bioreactors were treated with opposite ferric dosing strategies: one underwent five consecutive cycles of shock precipitation of hydrogen sulfide, reducing peak levels from ~400 mg/L to <40 mg/L; the other involved the application of a more continuous and fine-tuned dosing strategy to manage sulfide concentrations across different stepwise levels: 400–300, 300–200, 200–100, and 100 to <10 mg/L. A third bioreactor, without iron addition, stabilized at ~250 mg/L H2S and 10,000 ppm in biogas over 140 days. A detailed sulfur mass balance enabled analysis of sulfate-to-sulfide conversion rates, Henry’s con stants between liquid and gas phases, and Fe3+/S2− molar ratios for both strategies. Values of 0.99 and 0.65 were obtained, matching with the stoichiometry of FeS during shock precipitation and FeS + S0 and/or Fe2S3 in stepwise precipitation, respectively. High-throughput sequencing of the 16S rRNA gene revealed subtle yet significant changes in the microbial community structures within bioreactors subjected to high sulfate loads and iron addition. Furthermore, the authors characterized the late-stage microbial response following methanogenic process inhibition and the cessation of biogas production.Elsevier2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/208302214-714410.1016/j.jwpe.2025.108405Journal of Water Process Engineering. 2025 77reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-11-27T10:21:43Zoai:ucacris:123456789/20830instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-11-27 10:21:43.662Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
spellingShingle Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
Girardi, Valentina
BIOGAS
SULFATOS
SULFURO
title_short Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_full Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_fullStr Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_full_unstemmed Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_sort Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
dc.creator.none.fl_str_mv Girardi, Valentina
Tondo, María Laura
Balaban, Cecilia Lucía
Herrero, María Sol
Olivera, Camila
Salvatierra, Lucas Matías
author Girardi, Valentina
author_facet Girardi, Valentina
Tondo, María Laura
Balaban, Cecilia Lucía
Herrero, María Sol
Olivera, Camila
Salvatierra, Lucas Matías
author_role author
author2 Tondo, María Laura
Balaban, Cecilia Lucía
Herrero, María Sol
Olivera, Camila
Salvatierra, Lucas Matías
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOGAS
SULFATOS
SULFURO
topic BIOGAS
SULFATOS
SULFURO
dc.description.none.fl_txt_mv Fil: Girardi, Valentina. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Girardi, Valentina. CONICET; Argentina
Fil: Tondo, María Laura. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Tondo, María Laura. CONICET; Argentina
Fil: Balaban, Cecilia Lucía. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Balaban, Cecilia Lucía. CONICET; Argentina
Fil: Herrero, María Sol. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Olivera, Camila. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Olivera, Camila. CONICET; Argentina
Fil: Salvatierra, Lucas Matías. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
Fil: Salvatierra, Lucas Matías. CONICET; Argentina
This article presents an original and detailed study of semi continuous lab-scale anaerobic bioreactors subjected to Sulfate Loads Rates (SLRs) >130 mgSO4 2− /day.Lreactor. At these sulfate inputs, hydrogen sulfide yield by bacterial conversion can reach concentrations above 400 mg/L in the liquid phase and more than 25,000 ppm in gas phase, respectively. Both values comprise significant technical challenges in biogas plants as well as health and environmental concerns. In order to control sulfide in liquid phase, two bioreactors were treated with opposite ferric dosing strategies: one underwent five consecutive cycles of shock precipitation of hydrogen sulfide, reducing peak levels from ~400 mg/L to <40 mg/L; the other involved the application of a more continuous and fine-tuned dosing strategy to manage sulfide concentrations across different stepwise levels: 400–300, 300–200, 200–100, and 100 to <10 mg/L. A third bioreactor, without iron addition, stabilized at ~250 mg/L H2S and 10,000 ppm in biogas over 140 days. A detailed sulfur mass balance enabled analysis of sulfate-to-sulfide conversion rates, Henry’s con stants between liquid and gas phases, and Fe3+/S2− molar ratios for both strategies. Values of 0.99 and 0.65 were obtained, matching with the stoichiometry of FeS during shock precipitation and FeS + S0 and/or Fe2S3 in stepwise precipitation, respectively. High-throughput sequencing of the 16S rRNA gene revealed subtle yet significant changes in the microbial community structures within bioreactors subjected to high sulfate loads and iron addition. Furthermore, the authors characterized the late-stage microbial response following methanogenic process inhibition and the cessation of biogas production.
description Fil: Girardi, Valentina. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario; Argentina
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/20830
2214-7144
10.1016/j.jwpe.2025.108405
url https://repositorio.uca.edu.ar/handle/123456789/20830
identifier_str_mv 2214-7144
10.1016/j.jwpe.2025.108405
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Water Process Engineering. 2025 77
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 12.50043

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